AbstractApplication of controlled-release urea (CRU) improves crop yield and nitrogen use efficiency (NUE) compared with conventional urea. However, the effectiveness of CRU differs with fertilization placement. A two site-year field experiment was carried out in fluvo-aquic soil in central China to study the effects of two N sources (CRU and urea) and three fertilization placements (band application between two corn rows at 0, 5, and 15 cm soil depths) on fresh ear yield and NUE of sweet corn. The soil inorganic N (NO3−-N and NH4+-N) concentrations at the soil layers of 0–20 cm and 20–40 cm, root morphology characteristics and leaf physiological functions were also measured during the sweet corn growth period. Results showed that the deep placement of CRU at 15 cm soil depth significantly increased the sweet corn fresh ear yield, total N uptake, and NUE by 6.3%–13.4%, 27.9%–39.5%, and 82.9%–140.1%, respectively compared with CRU application at 0 cm depth. Deep placement of CRU at 15 cm also increased the root morphology traits, gas exchange attributes, and soil NO3−-N and NH4+-N concentrations in 0–20 cm and 20–40 cm layer, especially during later crop growth stages. However, the different N placements exerted non-significant effects on NUE and fresh ear yield when urea was applied as the N source. In crux, deep CRU placement instead of urea at 15 cm depth can effectively improve fresh ear yield and NUE of sweet corn in fluvo-aquic soil because of higher root growth, better leaf physiological functions and increased availability of soil NO3−-N and NH4+-N.