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Improved ethanol production from cheese whey, whey powder, and sugar beet molasses by “Vitreoscilla hemoglobin expressing” Escherichia coli

descriptionPublicationkeyboard_double_arrow_right Article , Journal , Other literature type 03 Apr 2014Publisher:Informa UK LimitedJournal:Bioscience, Biotechnology, and Biochemistry, volume 78, pages 687-694 (issn: 0916-8451, eissn: 1347-6947,

Authors: Akbas, Meltem Yesilcimen; Sar, Taner; Ozcelik, Busra;

doi: 10.1080/09168451.2014.896734

pmid: 25036968

Improved ethanol production from cheese whey, whey powder, and sugar beet molasses by “Vitreoscilla hemoglobin expressing” Escherichia coli

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Abstract

Abstract This work investigated the improvement of ethanol production by engineered ethanologenic Escherichia coli to express the hemoglobin from the bacterium Vitreoscilla (VHb). Ethanologenic E. coli strain FBR5 and FBR5 transformed with the VHb gene in two constructs (strains TS3 and TS4) were grown in cheese whey (CW) medium at small and large scales, at both high and low aeration, or with whey powder (WP) or sugar beet molasses hydrolysate (SBMH) media at large scale and low aeration. Culture pH, cell growth, VHb levels, and ethanol production were evaluated after 48 h. VHb expression in TS3 and TS4 enhanced their ethanol production in CW (21–419%), in WP (17–362%), or in SBMH (48–118%) media. This work extends the findings that “VHb technology” may be useful for improving the production of ethanol from waste and byproducts of various sources.

Related Organizations

Gebze Technical University
Turkey

Keywords

Ethanol, Truncated Hemoglobins, Bioreactors, Transformation, Genetic, Bacterial Proteins, Cheese, Escherichia coli, Molasses, Beta vulgaris, Genetic Engineering

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Farklı gıda işleme atıklarından biyoetanol üretiminde Vitreoscilla hemoglobininin kullanım potansiyelinin araştırılması
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