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Reproducible DNA fingerprinting with the random amplified polymorphic DNA (RAPD) method

Much interest has recently arisen in methods for DNA fingerprinting based on the polymerase chain reaction (PCR). Among these, the Random Amplified Polymorphic DNA (RAPD) method, developed by Williams eta]. (1), is currently receiving particular attention (2) because of its extreme simplicity and requirement for minimal amounts of genomic DNA. The basic strategy involves the PCR amplification of random fragments of genomic DNA with single or multiple (3) primers of arbitrary sequence. Polymorphism between individuals (or strains) is detected as differences between the pattern of DNA fragments amplified from the different DNAs using a given primer(s).
- University of Perugia Italy
- Sapienza University of Rome Italy
- University of L'Aquila Italy
Polymorphism, Genetic, Ethanol, Animals, DNA, DNA Fingerprinting, Polymerase Chain Reaction, Rats
Polymorphism, Genetic, Ethanol, Animals, DNA, DNA Fingerprinting, Polymerase Chain Reaction, Rats
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).143 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 1% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 1%
