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Protein Science
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Protein Science
Article . 2002 . Peer-reviewed
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Article . 2002
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Protein Science
Article . 2002
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Effects of molecular crowding by saccharides on α‐chymotrypsin dimerization

Authors: Patel, C. N.; Noble, S. M.; Weatherly, G. T.; Tripathy, A.; Winzor, D. J.; Pielak, G. J.;

Effects of molecular crowding by saccharides on α‐chymotrypsin dimerization

Abstract

AbstractGiven the importance of protein complexes as therapeutic targets, it is necessary to understand the physical chemistry of these interactions under the crowded conditions that exist in cells. We have used sedimentation equilibrium to quantify the enhancement of the reversible homodimerization of α‐chymotrypsin by high concentrations of the osmolytes glucose, sucrose, and raffinose. In an attempt to rationalize the osmolyte‐mediated stabilization of the α‐chymotrypsin homodimer, we have used models based on binding interactions (transfer‐free energy analysis) and steric interactions (excluded volume theory) to predict the stabilization. Although transfer‐free energy analysis predicts reasonably well the relatively small stabilization observed for complex formation between cytochrome c and cytochrome c peroxidase, as well as that between bobtail quail lysozyme and a monoclonal Fab fragment, it underestimates the sugar‐mediated stabilization of the α‐chymotrypsin dimer. Although predictions based on excluded volume theory overestimate the stabilization, it would seem that a major determinant in the observed stabilization of the α‐chymotrypsin homodimer is the thermodynamic nonideality arising from molecular crowding by the three small sugars.

Country
Australia
Keywords

Biochemistry & Molecular Biology, Sucrose, Virial-coefficients, Excluded-volume, Centrifugation, 270100 Biochemistry and Cell Biology, Association, C1, Raffinose, Thermodynamic Nonideality, Chymotrypsin, Cytochrome-c-peroxidase, Bobwhite Quail Lysozyme, 780105 Biological sciences, Temperature, Proteins, Sedimentation Equilibrium, 541, Stabilization, Kinetics, Glucose, Energy Transfer, Molecular Crowding, Dimerization, Protein Binding

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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
47
Top 10%
Top 10%
Top 10%
bronze
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