Abstract There is a growing need for drop-in biofuels for gas turbines for enhanced energy security and sustainability. Several fuels are currently being developed and tested to reduce dependency on fossil fuels while maintaining performance, particularly in the aviation industry. The transition from traditional fossil fuels to sustainable biofuels is much desired for reducing the rapidly rising CO2 levels in the environment. This requires biofuels to be drop-in ready, where there are no adverse effects on performance and emissions upon replacement. In this study, the performance and emissions of four different aviation drop-in biofuels were evaluated. They include UOP HEFA-SPK, Gevo ATJ, Amyris Farnesane, and SB-JP-8. These aviation biofuels are currently being produced and tested to be ready for full or partial drop-in fuels as the replacement of traditional jet fuels. The characteristic performance of each fuel from the prevaporized liquid fuels was performed in a high-intensity (20 MW/m3-atm) reverse flow combustor. The NO emissions showed near unity ppm levels for each of the fuels examined with a minimum at an equivalence ratio of ∼0.6, while CO levels were in the range of 1000–1300 ppm depending on the fuel at an equivalence ratio between 0.75 and 0.8. For an equivalence ratio range between 0.4 and 0.6, NO and CO emissions remained very low (between 1–2 ppm NO and 2400–2900 ppm CO) depending on the fuel. The examined biofuels did not show any instability over a wide range of equivalence ratios from lean to near stoichiometric condition. These results provide promising results on the behavior of these drop-in aviation biofuels for use in high-intensity gas turbine combustors providing stability and cleaner performance without any modification to the combustor design.