Abstract The paper discusses changes in the hydrological regime of high mountain Lake Morskie Oko located in the Tatra Mountains, in the Tatra Mountains National Park, a UNESCO biosphere reserve (MaB). According to the research conducted in the years 1971–2015, its water stages decreased by 3.5 cm·dec−1, mean annual water temperature increased by 0.3°C·dec−1 and the duration of ice phenomena and ice cover was reduced by 10 day·dec−1. No considerable changes in maximum values of ice cover thickness were recorded. Such tendencies are primarily caused by long-term changes in climatic conditions – air temperature and atmospheric precipitation. The hydrological regime of the lake was also determined by changes in land use in the lake’s catchment and its location in high mountains.