The Danube, the Sava and the Tisa rivers belong to the Black Sea river basin and they flow through many European countries, while in Serbia they flow through the Pannonian flatland. The water quality of these rivers, which is affected by complex anthropogenic activities and natural factors, is one of important factors that are taken into account in the evaluation of development sustainability of this region. Using the correlation analysis in this paper we tested the impact of air temperature as a natural factor on certain parameters indicating the water quality. A significant correlation was found between air temperature and total nitrogen oxides in the Danube of up to -0.744, namely of -0.740 on the Tisa and -0.299 on the Sava. A significant correlation was found in electrical conductivity - up to -0.793 on the Danube, -0.226 on the Tisa, and 0.380 on the Sava. Correlation links were found between air temperature and O2, BOD, as well as suspended matters saturation percentage, but their correlation values are significantly lower. Based on the results of the research it was concluded that air temperature had low effect on changes in water quality during the year and that anthropogenic impact was far more dominant.