The Shannon-Wiener Index (H′), AZTI’s Marine Biotic Index (AMBI) and multivariate AMBI (M-AMBI) were applied as a comparative applicability in assessing the ecological quality status of sediment (EcoQ) of eight an organic shrimp farming ponds (OSFP), Ca Mau province. There were obvious differences between the evaluation results of three indices in the eight OSFPs through three seasons. The EcoQ given by the AMBI and M-AMBI were higher than that given by the H’ index. These indicated that H’ index may also be more sensitive to environment disturbances than the AMBI and M-AMBI. Furthermore, the EcoQ given by the M-AMBI was a neutralization between that given by the H’ and AMBI indices. Because there are no environmental variables data available in this study, the H′ index was really more sensitive to environment disturbances than the AMBI and M-AMBI, that have yet to be fully elucidated. A analyses three indices with environmental variables gradient data also need further investigation to potentially increase the precise answer of this issue.