The CO2 storage capacity estimated at the regional scale provides a preliminary recognition of as far as the possible uses of CO2 sequestration as an option of emission reduction. There has been presented the estimation of CO2 storage capacity for aquifers of Lower Cretaceous, Lower Jurassic and Lower Triassic of Polish Lowland. The Lower Jurassic deposits exhibit the greatest CO2 storage capacity of all the horizons, storage capacity of the Lower Triassic deposits is lower, the smallest capacity was estimated for the Lower Cretaceous deposits. The use of specific capacity maps for carbon dioxide in aquifers has been suggested in order to estimate the potential on national and basin scale as well as the search for prospective areas for CO2 storage.