Cudlín, Pavel et. al.- 16 páginas.- Ilustraciones.- Se acompaña suplemento de 3 páginas.- © The autors 2017. Open Access under Creative Commons by Attribution Licence. Use, distribution and reproduction are unrestricted. Authors and original publication must be credited. A growing body of evidence suggests that processes of upward treeline expansion and shifts in vegetation zones may occur in response to climate change. However, such shifts can be limited by a variety of non-climatic factors, such as nutrient availability, soil conditions, landscape fragmentation and some species-specific traits. Many changes in species distributions have been observed, although no evidence of complete community replacement has been registered yet. Climatic signals are often confounded with the effects of human activity, for example, forest encroachment at the treeline owing to the coupled effect of climate change and highland pasture abandonment. Data on the treeline ecotone, barriers to the expected treeline or dominant tree species shifts due to climate and land use change, and their possible impacts on biodiversity in 11 mountain areas of interest, from Italy to Norway and from Spain to Bulgaria, are reported. We investigated the role of environmental conditions on treeline ecotone features with a focus on treeline shift. The results showed that treeline altitude and the altitudinal width of the treeline ecotone, as well as the significance of climatic and soil parameters as barriers against tree species shift, significantly decreased with increasing latitude. However, the largest part of the commonly observed variability in mountain vegetation near the treeline in Europe seems to be caused by geomorphological, geological, pedological and microclimatic variability in combination with different land use history and present socio-economic relations. This paper is based firstly upon work from the COST Action ES 1203 SENSFOR, supported by COST (European Cooperation in Science and Technology; www.cost.eu). This international work was further supported by projects granted by the Ministry of Education Youth and Sports of the Czech Republic, grant NPU I LO1415 and LD 14039, by the agency APVV SR under projects APVV-14-0086 and APVV-15-0270. We acknowledge the E-OBS dataset from the EU-FP6 project ENSEMBLES (http://ensembles-eu.metoffice.com) and the data providers in the ECA&D project (www.ecad.eu) Peer reviewed