This study proposes an innovative approach to reduce temperature fluctuations in refrigerated transport during loading and unloading, aiming to minimize food waste and optimize energy consumption in the food supply chain. The solution involves integrating Peltier cells into secondary and tertiary packaging to improve system efficiency and minimize temperature variations. Four distinct tests were conducted: a reference test, continuous Peltier system operation, and two intermittent cooling tests for the hot side of the cells. The results highlight the effectiveness of this approach, particularly in the fourth test where the average final food temperature decreased from 3.2 °C (reference test) to 2.8 °C. Integrating Peltier cells into packaging shows potential benefits in minimizing food waste, reducing energy consumption, and associated emissions during refrigerated transport. This research contributes to the sustainable design and manufacturing of packaging systems, specifically in the context of refrigerated transport. By maintaining a consistent temperature environment during the critical loading and unloading phases, incorporating Peltier cells enhances the overall performance and efficiency of refrigerated transport system. These results point out the significance of exploring innovative solutions for sustainable food preservation and the decrease of waste all along the food supply chain.