The use of new sources of biomass residues for energy purposes in Europe is crucial for increasing the share of renewable energy sources and the limitation of carbon dioxide emissions. The residues coming from regular pruning of permanent crops are an alternative to conventional fuels. The paper is focused on the assessment of European pruning potentials in European Union (EU28) in line with the nomenclature of territorial units (NUTs) at NUTs0, NUTs2 and NUTs3 level. The assessment indicates that the yearly theoretical and technical potential of that biomass is 13.67 MtDM (or 252.0 PJ·yr−1) and 12.51 MtDM (or 230.6 PJ·yr−1), respectively. The economic potential has been assessed based on different management or exploitation models: management of pruning as a waste, self-consumption, and demand-driven mobilisation by consumption centres at small, medium and large scales. The utilisation of pruning when gathering is compulsory coincides with the technical potential. Under self-consumption, up to 10.98 MtDM per year could be effectively mobilised (202.3 PJ·yr−1). The creation of new value chains for delivery of pruning biomass ranges 7.30 to 8.69 MtDM per year (from 134.5 to 160.2 PJ·yr−1). When applying further constraints related to other existing uses the implementation of the potential further descends, ranging from 6.18 to 10.66 MtDM per year (from 113.9 to 196.4 PJ·yr−1). The analysis shows that the amount of available pruning residues is regionally scattered; however, most of them (ca. 80%) are located in the Mediterranean area.