Biofuel consists of non-fossil fuel derived from the organic biomass of renewable resources, including plants, animals, microorganisms, and waste. Energy derived from biofuel is known as bioenergy. The reserve of fossil fuels is now limited and continuing to decrease, while at the same time demand for energy is increasing. In order to overcome this scarcity, it is vital for human beings to transfer their dependency on fossil fuels to alternative types of fuel, including biofuels, which are effective methods of fulfilling present and future demands. The current review therefore focusses on second-generation lignocellulosic biofuels obtained from non-edible plant biomass (i.e., cellulose, lignin, hemi-celluloses, non-food material) in a more sustainable manner. The conversion of lignocellulosic feedstock is an important step during biofuel production. It is, however, important to note that, as a result of various technical restrictions, biofuel production is not presently cost efficient, thus leading to the need for improvement in the methods employed. There remain a number of challenges for the process of biofuel production, including cost effectiveness and the limitations of various technologies employed. This leads to a vital need for ongoing and enhanced research and development, to ensure market level availability of lignocellulosic biofuel.