Preparation of TiO2 using the hydrothermal treatment in NH4OH solution and subsequent thermal heating at 500–700 °C in Ar was performed in order to introduce some titania surface defects. The highest amount of oxygen vacancies and Ti3+ surface defects were observed for a sample heat-treated at 500 °C. The presence of these surface defects enhanced photocatalytic properties of titania towards the deactivation of two bacteria species, E. coli and S. epidermidis, under artificial solar lamp irradiation. Further modification of TiO2 was targeted towards the doping of Cu species. Cu doping was realized through the impregnation of the titania surface by Cu species supplied from various copper salts in an aqueous solution and the subsequent heating at 500 °C in Ar. The following precursors were used as a source of Cu: CuSO4, CuNO3 or Cu(CH3COO)2. Cu doping was performed for raw TiO2 after a hydrothermal process with and without NH4OH addition. The obtained results indicate that Cu species were deposited on the titania surface defects in the case of reduced TiO2, but on the TiO2 without NH4OH modification, Cu species were attached through the titania adsorbed hydroxyl groups. Cu doping on TiO2 increased the absorption of light in the visible range. Rapid inactivation of E. coli within 30 min was obtained for the ammonia-reduced TiO2 heated at 500 °C and TiO2 doped with Cu from CuSO4 solution. Photocatalytic deactivation of S. epidermidis was greatly enhanced through Cu doping on TiO2. Impregnation of TiO2 with CuSO4 was the most effective for inactivation of both E. coli and S. epidermidis.