Grasslands have a natural capacity to decrease air pollution and a positive impact on human life. However, their maintenance requires adequate irrigation, which is difficult to apply in many regions where drought and high temperatures are frequent. Therefore, the selection of grass species more tolerant to a lack of irrigation is a fundamental criterion for green space planification. This study compared responses to deficit irrigation of different turfgrass mixtures: a C4 turfgrass mixture, Cynodon dactylon-Brachypodium distachyon (A), a C4 turfgrass mixture, Buchloe dactyloides-Brachypodium distachyon (B), and a standard C3 mixture formed by Lolium perenne-Festuca arundinacea-Poa pratensis (C). Three different irrigation regimes were assayed, full irrigated to 100% (FI-100), deficit irrigated to 75% (DI-75), and deficit irrigated to 50% (DI-50) of container capacity. Biomass, normalized difference vegetation index (NDVI), green area (GA), and greener area (GGA) vegetation indices were measured. Irrigation significantly affected the NDVI, biomass, GA, and GGA. The most severe condition in terms of decreasing biomass and vegetation indices was DI-50. Both mixtures (A) and (B) exhibited higher biomass, NDVI, GA, and GGA than the standard under deficit irrigation. This study highlights the superiority of (A) mixture under deficit irrigation, which showed similar values of biomass and vegetation indices under full irrigated and deficit irrigated (DI-75) container capacities.