The purpose of this study was to evaluate the streamflow and water quality (SS, T-N, and T-P) interaction of the Nakdong river basin (23,609.3 km2) by simulating dam and weir operation scenarios using the Soil and Water Assessment Tool (SWAT). The operation scenarios tested were dam control (Scenario 1), dam control and weir gate control (Scenario 2), dam control and sequential release of the weirs with a one-month interval between each weir (Scenario 3), dam control and weir gate full open (Scenario 4), dam control and weir gate sequential full open (Scenario 5), weir gate control (Scenario 6), weir gate full open (Scenario 7), and weir gate sequential full open (Scenario 8). Before evaluation, the SWAT was calibrated and validated using 13 years (2005–2017) of daily multi-purpose dam inflow data from five locations ((Andong Dam (ADD), Imha Dam (IHD), Hapcheon Dam (HCD), Namkang Dam (NKD), and Milyang Dam (MYD))multi-function weir inflow data from seven locations (Sangju Weir (SJW), Gumi Weir (GMW), Chilgok Weir (CGW), Gangjeong-Goryeong Weir (GJW), Dalseong Weir (DSW), Hapcheon-Changnyeong Weir (HCW), and Changnyeong-Haman Weir (HAW)), and monthly water quality monitoring data from six locations (Andong-4 (AD-4), Sangju (SJ-2), Waegwan (WG), Hapcheon (HC), Namkang-4 (NK-4), and Mulgeum (MG). For the dam inflows and dam storage, the Nash-Sutcliffe efficiency (NSE) was 0.59~0.78, and the coefficient of determination (R2) was 0.71~0.90. For water quality, the R2 values of SS, T-N, and T-P were 0.58~0.83, 0.53~0.68, and 0.56~0.79, respectively. For the eight dam and weir release scenarios suggested by the Ministry of Environment, Scenarios 4 and 8 exhibited water quality improvement effects compared to the observed data.