The development of urban rail transit is of great significance to the sustainable development of cities. However, the formulation of public norms of the urban rail transit train operation scheme lacks the corresponding theoretical support, rationality and scientificity. Therefore, based on the research on passenger flow conditions, this paper establishes an optimization model of express train operation and uses a heuristic genetic algorithm to solve it. In addition, this paper takes the Beijing Changping Line as an example to conduct empirical research. Results show that the optimization model established reduces the operation cost of operating enterprises, improves the capacity of urban rail transit, reduces travel time and maintains the full load rate of trains at a more comfortable level. This will improve the attractiveness of urban rail transit and promote benign interaction between operators and passengers. It will also provide a theoretical basis for formulating public norms of transit train operation, make it more scientific and reasonable and promote the development of urban rail transit.