This article presents and discusses a pilot test concerning the environmental impacts assessment of organizations. The study was conducted in a production site of a world leading group in the plastic packaging industry. The purpose was to deepen the knowledge of the methodologies currently available and also of the benefits and the difficulties related to their practical implementation. The analysis was conducted in the period 2018–2019, in line with ISO 14044 and ISO/TS 14072 standards and therefore inspired by the organizational life cycle assessment (O-LCA) procedures and standards. The O-LCA test application has proved useful in detecting a number of environmental hotspots usually not detected by other life cycle based approaches, on which the organization should focus to undertake improvement actions, both internal and external (e.g., the category “employees commuting”). The experience gained in the production site under study also led to the replication of the assessment approach to other facilities around the world and for informing corporate sustainability reporting activities (e.g., Global Reporting Initiative and Carbon Disclosure Project reporting standards). Such results can provide methodological and practical insights to both scholars and practitioners in that field of activities.