According to the European Commission, green infrastructure and spatial connectivity concerning the provision of ecosystem services are strictly related to the conceptual category of ecological networks. In particular, regional and urban planning processes should adequately manage, improve and monitor the effectiveness of green infrastructures as ecological networks which provide ecosystem services and the spatial connectivity of such systems. Building on a methodological approach defined in previous studies, this article aims at identifying ecological corridors through a least-cost path model with reference to the spatial layout of a set of protected areas. Moreover, such a methodological approach is implemented in the context of the Sardinian region to map ecological corridors, which form, together with protected areas, a network representing the spatial framework of regional green infrastructure. Finally, the study discusses the relation between ecological corridors and the spatial taxonomy of the landscape components featured by environmental relevance, identified by the Regional Landscape Plan, through multiple linear regression analysis, in order to assess if, and to what extent, the present regional spatial zoning code can be used as a basis to implement regulations aimed at protecting ecological corridors. This methodological approach is relevant to defining planning policies and measures to strengthen the operational capacity and effectiveness of regional networks of protected areas through the protection and the improvement of the spatial framework of ecological corridors.