A Before–Intermediate–After Multiple Sites (BIAMS) analysis, namely a modified version of the Before–After–Control–Impact (BACI) approach, was used to evaluate the possible effects of fishery management measures implemented in the Pomo/Jabuka Pits area, a historically highly exploited ground for Italian and Croatian fisheries, whose impact may have contributed over the years to the modification of the ecosystem. Since 2015, the area was subject to fishing regulations changing the type of restrictions over time and space, until the definitive establishment in 2018 of a Fishery Restricted Area. These changes in the regulatory regime result in complex signals to be interpreted. The analysis was carried out on abundance indices (i.e., kg/km2 and N/km2) of five commercially or ecologically relevant species, obtained in the period 2012–2019 from two annual trawl surveys. BIAMS was based on the selection of a Closure factor, declined in three levels (i.e., BEFORE/INTERMEDIATE/AFTER) and accounting for regulation changes in time, and on three adjacent strata (i.e., “A”, “B”, and “ext ITA”) a posteriori determined according to the latest regulations. BIAMS allowed us to identify early effects (i.e., changes in abundances), overcoming the unavailability of a proper independent control site; furthermore, the selection of adjacent strata allowed the inference of possible interactions among them.