Ecosystems are experiencing significant pressure from human activities, with 1 million species at risk of extinction. This is threatening to undermine the resilience of ecosystems, which provide multiple benefits to support human existence and are essential for the support of life on Earth. A number of conceptual frameworks have been developed as a guide for the assessment of ecosystem services (ESs) and nature’s contributions to people (NCPs), including Millennium Ecosystems Assessment, The Economics of Ecosystems and Biodiversity report, France’s National Ecosystems Assessment, the Common International Classification of Ecosystem Services, and the Global Assessment of the Intergovernmental Science-Policy Platform on Biodiversity and Ecosystem Services. In this paper, we compare the existing conceptual frameworks for the assessment of ESs and NCPs and derive a unified structural framework. Several indicators for characterizing the ESs/NCPs provided are selected and integrated through normalization. On this basis, and enriched by a number of culture-specific indicators, we conduct a mapping exercise illustrating the ES/NCP provision for the whole of France in a spatially explicit form based on a 1 × 1 km scale. Finally, we generate integrated maps depicting distribution patterns of different services and contributions across the landscapes of France focusing on economic, social and ecological dimensions. The results indicate that a non-monetary assessment of the complexity and diversity of NCPs is feasible and presents tangible advantages as compared to monetary frameworks. The paper concludes that provisioning, regulating and cultural services and contributions are geographically unevenly distributed and further analysis is required to assess the degree of complementarity, feedback loops and tipping points among different services. Our pilot research clearly illustrated the feasibility of conducting a highly disaggregated multidimensional assessment of ESs/NCPs at the national scale to inform decision making.