Agility has a special place in the start-up Industry 4.0 era. More research is required to properly comprehend the agile implications of start-up Industry 4.0 regarding the impact of digitization on the economy, the environment, and society. Investigating the effects of start-up 4.0 agility is still in its early stages. The current study simulates the variables impacting agility in start-up activities in Industry 4.0 to tackle this problem. In addition to the pre-arranged interview, a closed-ended questionnaire was used to gather information. In the context of start-up operations 4.0, the MICMAC technique is used to evaluate and categorize the components that contribute to agility in order to comprehend their interconnections. The research identified eleven characteristics of facilitating agility in start-up operations 4.0. Industry 4.0 concepts have significantly influenced large organizations but deploying agility in start-up 4.0 has been less visible. Hence, this study presents an innovative approach to incorporating agility in modern start-up operations. The significance of artificial intelligence, cloud computing, network and connectivity, technology, and digital twin in this context is evident. The research provides important light on the elements that contribute to the successful use of agility in start-up 4.0, offering useful insights for stakeholders and academics.