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Article . 1990

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In vitro effects of ethanol and acetaldehyde on lymphoid cells as determined by 31P NMR spectroscopy.

descriptionPublicationkeyboard_double_arrow_right Article 07 Jun 1990 English Journal:Biomedica biochimica acta, volume 48 (issn: 0232-766X,

Authors: A S, Walia; E W, Lamon;

pmid: 2634956

In vitro effects of ethanol and acetaldehyde on lymphoid cells as determined by 31P NMR spectroscopy.

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Abstract

We have used NMR spectroscopy to test the direct in vitro effects of ethanol and its metabolite acetaldehyde on a human leukemic T cell line (Molt-4) and normal murine spleen cells. The metabolic status of phosphate metabolites (phosphomonoesters including sugar phosphate, inorganic phosphate and ATP's) in cell suspension was monitored by 31P NMR spectroscopy. Spectral changes were observed in the intensity of inorganic phosphate and ATP. Addition of high concentrations of ethanol (400-1600 mM) to Molt-4 cells resulted in very little spectral change. However, the addition of 40 microM acetaldehyde resulted in substantially increased inorganic phosphate (Pi) signals, and decreased phosphomonoesters and ATP signals. Similar changes, but to a lesser degree, were observed with normal mouse spleen cells.

Keywords

Male, Mice, Inbred C3H, Magnetic Resonance Spectroscopy, Ethanol, Cell Survival, T-Lymphocytes, Phosphorus, Acetaldehyde, Precursor Cell Lymphoblastic Leukemia-Lymphoma, Cell Line, Mice, Adenosine Triphosphate, Mice, Inbred CBA, Tumor Cells, Cultured, Animals, Humans, Female, Spleen

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