The effects of short-term (4 years) tillage (hereafter called reverse tillage [RT]) of land previously under long-term (29 or 30 years) no-till (NT), with straw management (straw removed [SRem] and straw retained [SRet]) and N fertilizer rate (0, 50 and 100 kg N ha-1 in SRet, and 0 kg N ha-1 in SRem plots) were determined in autumn 2011 on ammonium-N, nitrate-N and extractable P in the 0-7.5, 7.5-15 and 15-20 cm soil layers at Breton (Gray Luvisol [Typic Cryoboralf] loam) and Ellerslie (Black Chernozem [Albic Argicryoll] loam), Alberta, Canada. There was no significant effect of RT and straw on ammonium-N, nitrate-N and extractable P in soil. Ammonium-N in soil increased significantly (but small) with N rate in many cases at both sites. Nitrate-N in soil increased with increasing N rate from 0 to 100 kg N ha-1 rate at Ellerslie, and up to 50 kg N ha-1 rate at Breton. Etractable P in soil decreased markedly with increasing N rate up to 100 kg N ha-1 at Breton and up to 50 kg N ha-1 at Ellerslie. In summary, increased N fertilizer rates were usually associated with decreased extractable P and increased nitrate-N in soil, but RT and straw had no effect on these nutrients in soil.