During the last 30 years, a collection of morphological barley mutants has been developed in Fiorenzuola d’Arda, Italy, as part of the international effort directed to the conservation of genetic stocks, to ensure the global availability and the correct maintenance of these genetic materials. The collection is comprehensive of stem, leaf, ear, flower, awn and grain mutants. Near isogenic lines (NILs) useful for genetic analysis and to study the effect of mutation on agronomic performance have been developed. New different hooded mutants have been obtained in rare outcrosses of the flower in the hood. The development of double and triple mutants obtained by intercrossing simple mutants has been done and will continue. The collection is continuously implemented by including new mutants through a collaborative work with the other germplasm banks and represents a unique source of alleles for better understanding the role of genes involved in plant architecture.