A direct-transfer polymerization model explains how the multiple profilin-binding sites in the actoclampin motor promote rapid actin-based motility
Copyright policy )A direct-transfer polymerization model explains how the multiple profilin-binding sites in the actoclampin motor promote rapid actin-based motility
The high actin-based motility rates observed in nonmuscle cells require the per-second addition of 400-500 monomers to the barbed ends of growing actin filaments. The chief polymerization-competent species is profilin.actin.ATP (present at 5-40 microM intracellular concentrations), whereas G-actin.ATP is much less abundant ( approximately 0.1-1 microM). While earlier studies unambiguously demonstrated that profilin.actin is highly concentrated within the polymerization zone, profilin-actin localization on the motile surface cannot increase the local solution-phase concentration of polymerizable actin. To explain these high rates of actin polymerization, we present and analyze a novel polymerization model in which monomers are directly transferred to growing filament ends in the actoclampin motor. This direct-transfer polymerization mechanism endows the polymerization zone with properties unavailable to bulk-phase actin monomers, and our model also indicates why profilin is the ideal mobile carrier for actin monomers.
- Florida Southern College United States
Binding Sites, Polymers, Movement, Microfilament Proteins, Models, Theoretical, Models, Biological, Actins, Recombinant Proteins, Kinetics, Profilins, Contractile Proteins, COS Cells, Chlorocebus aethiops, Mutagenesis, Site-Directed, Animals, Point Mutation, Shigella
Binding Sites, Polymers, Movement, Microfilament Proteins, Models, Theoretical, Models, Biological, Actins, Recombinant Proteins, Kinetics, Profilins, Contractile Proteins, COS Cells, Chlorocebus aethiops, Mutagenesis, Site-Directed, Animals, Point Mutation, Shigella
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