Mutation and Expression Analysis of Corticotropin-Releasing Factor 1 Receptor in Adrenocorticotropin-Secreting Pituitary Adenomas1
Copyright policy )Mutation and Expression Analysis of Corticotropin-Releasing Factor 1 Receptor in Adrenocorticotropin-Secreting Pituitary Adenomas1
The present study was designed to investigate a possible role of CRF1 receptors (CRF1-R) in the pathogenesis of Cushing’s disease. ACTH-secreting pituitary adenomas and nonsecreting pituitary adenomas have been analyzed for mutations in the CRF1-R gene by PCR and sequencing and been compared with the sequences of normal anterior pituitaries. No mutations affecting the CRF1-R protein have been found in all tumors analyzed. However, we found a significant overexpression of the CRF1-R messenger RNA in ACTH-secreting pituitary adenomas vs. inactive adenomas and normal pituitaries. We conclude that mutations of the CRF1-R are unlikely to be involved in Cushing’s disease. We suggest that the overexpression of the CRF1-R messenger RNA may be related to a disturbed receptor regulation in ACTH-secreting pituitary adenomas.
- University Hospital Magdeburg Germany
- Institute of Neurobiology Bulgaria
- Bulgarian Academy of Sciences Bulgaria
- University Medical Center Hamburg-Eppendorf Germany
Adenoma, Adult, Male, DNA, Complementary, Gene Expression, Polymerase Chain Reaction, Receptors, Corticotropin-Releasing Hormone, Adrenocorticotropic Hormone, Pituitary Gland, Anterior, Humans, Pituitary Neoplasms, RNA, Messenger, Child, Aged, Electrophoresis, Agar Gel, Sequence Analysis, DNA, Middle Aged, Mutation, Female
Adenoma, Adult, Male, DNA, Complementary, Gene Expression, Polymerase Chain Reaction, Receptors, Corticotropin-Releasing Hormone, Adrenocorticotropic Hormone, Pituitary Gland, Anterior, Humans, Pituitary Neoplasms, RNA, Messenger, Child, Aged, Electrophoresis, Agar Gel, Sequence Analysis, DNA, Middle Aged, Mutation, Female
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