Abstract In this work, we have developed a facile differential pressure route to prepare PET-based hollow fibers containing silver particles (Ag/PET hollow fibers). X-ray Diffraction and Scanning Electron Microscopy were used to confirm the existence of silver particles, and the results showed that 0.1–0.5 μm silver particles were incorporated in the inner side-wall of the hollow portion. Inductively Coupled Plasma Atomic Emission Spectrometer was applied to detect the release behavior of silver ions from various Ag/PET hollow fibers. And the results showed that the release behavior of silver ions was depended on time and the length of the fibers, which may be explained by the water uptake property of the Ag/PET hollow fibers.