In order to identify good commercial maize hybrids and population densities for huitlacoche production in greenhouse conditions, two experiments were carried out (2005 and 2006) in hydroponics. The first one included four hybrids in a population density of 62, 500 plants·ha-1. In second experiment seven hybrids were evaluated at 166, 666 plants·ha-1. Every hybrid was inoculated with its respective fungus strain, obtained of each hybrid. In both individual assays and in the combined analysis of two experiments, the best hybrid was 30G40 with production of 1,649.2 kg·ha-1, 7,923.30 kg·ha-1 and 4,786.20 kg·ha-1 (combined); derived from their percentage of incidence of 23.67, 52.41 and 38.04 %, and from their severity index of 52.48 and 47.21and 38.04, respectively. In the combined analysis, the yield of huitlacoche was higher at 166,666 plants·ha-1 (3,592.10 kg·ha-1) than at 62,500 plants·ha-1 (960.2 kg·ha-1), instead of the production of fungus per plant was similar in both densities (0.02 kg·planta-1) of 30G40 hybrid.