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European consumers are willing to pay more for “green” electricity, as they highly value renewable energy sources for the contribution to combating climate change. There is a push for getting higher levels of sustainability, leading to a differentiation of Europe‘s electricity market. In this differentiation, the large potential of wind energy is recognized. More specifically, North Sea countries prefer to plan wind arrays (far) out at sea. This article offers a review of the main arguments for offshore wind energy, described in comparison with its onshore counterpart. It is stated that offshore wind farms (OWFs) generate “dark green” electricity as they mitigate greenhouse gas emissions and contribute to the protection of (some) marine life. Applying an informational governance framework, this article further assesses whether this dark green message has been exploited through further differentiation of the electricity market, and provides an analysis of why this is not (yet) the case. It is concluded that the dominant discourse in onshore wind power development hinders a favorable ecological differentiation toward offshore wind power.

Biomass is considered one of the most important options in the transition to a sustainable energy system with reduced greenhouse gas (GHG) emissions and increased security of enegry supply. In order to facilitate this transition with targeted policies and implementation strategies, it is of vital importance to understand the economic benefits, uncertainties and risks of this transition. This article presents a quantification of the economic impacts on value added, employment shares and the trade balance as well as required biomass and avoided primary energy and greenhouse gases related to large scale biomass deployment on a country level (the Netherlands) for different future scenarios to 2030. This is done by using the macro-economic computable general equilibrium (CGE) model LEITAP, capable of quantifying direct and indirect effects of a bio-based economy combined with a spread sheet tool to address underlying technological details. Although the combined approach has limitations, the results of the projections show that substitution of fossil energy carriers by biomass, could have positive economic effects, as well as reducing GHG emissions and fossil energy requirement. Key factors to achieve these targets are enhanced technological development and the import of sustainable biomass resources to the Netherlands. (C) 2013 Elsevier Ltd. All rights reserved.

Activity pacing (AP) is a concept that is central to many chronic pain theories and treatments, yet there remains confusion regarding its definition and effects.To review the current knowledge concerning AP and integrate this knowledge in a manner that allows for a clear definition and useful directions for future research.A narrative review of the major theoretical approaches to AP and of the empirical evidence regarding the effects of AP interventions, followed by an integrative discussion.The concept of AP is derived from 2 main traditions: operant and energy conservation. Although there are common elements across these traditions, significant conceptual and practical differences exist, which has led to confusion. Little empirical evidence exists concerning the efficacy of AP as a treatment for chronic pain.Future research on AP should be based on a clear theoretical foundation, consider the context in which the AP behavior occurs and the type of pacing problem ("underactivity" vs. "overactivity"), and should examine the impact of AP treatment on multiple clinical outcomes. We provide a provisional definition of AP and specific recommendations that we believe will move the field forward.

ABSTRACTEnzymatic wheat gluten hydrolysis at high solid concentrations is advantageous from an environmental and economic point of view. However, increased wheat gluten concentrations result in a concentration effect with a decreased hydrolysis rate at constant enzyme‐to‐substrate ratios and a decreased maximum attainable degree of hydrolysis (DH%). We here identified the underlying factors causing the concentration effect. Wheat gluten was hydrolyzed at solid concentrations from 4.4% to 70%. The decreased hydrolysis rate was present at all solid concentrations and at any time of the reaction. Mass transfer limitations, enzyme inhibition and water activity were shown to not cause this hydrolysis rate limitation up to 50% solids. However, the hydrolysis rate limitation can be, at least partly, explained by a second‐order enzyme inactivation process. Furthermore, mass transfer impeded the hydrolysis above 60% solids. Addition of enzyme after 24 h at high solid concentrations scarcely increased the DH%, suggesting that the maximum attainable DH% decreases at high solid concentrations. Reduced enzyme activities caused by low water activities can explain this DH% limitation. Finally, a possible influence of the plastein reaction on the DH% limitation is discussed. Biotechnol. Bioeng. 2014;111: 1304–1312. © 2014 Wiley Periodicals, Inc.

Systemic delivery of therapeutic agents remains ineffective against diffuse intrinsic pontine glioma (DIPG), possibly due to an intact blood-brain-barrier (BBB) and to dose-limiting toxicity of systemic chemotherapeutic agents. Convection-enhanced delivery (CED) into the brainstem may provide an effective local delivery alternative for DIPG patients.The aim of this study is to develop a method to perform CED into the murine brainstem and to test this method using the chemotherapeutic agent carmustine (BiCNU). To this end, a newly designed murine CED catheter was tested in vitro and in vivo. After determination of safety and distribution, mice bearing VUMC-DIPG-3 and E98FM-DIPG brainstem tumors were treated with carmustine dissolved in DW 5% or carmustine dissolved in 10% ethanol.Our results show that CED into the murine brainstem is feasible and well tolerated by mice with and without brainstem tumors. CED of carmustine dissolved in 5% DW increased median survival of mice with VUMC-DIPG-3 and E98FM-DIPG tumors with 35% and 25% respectively. Dissolving carmustine in 10% ethanol further improved survival to 45% in mice with E98FM-DIPG tumors.Since genetically engineered and primary DIPG models are currently only available in mice, murine CED studies have clear advantages over CED studies in other animals.CED in the murine brainstem can be performed safely, is well tolerated and can be used to study efficacy of chemotherapeutic agents orthotopically. These results set the foundation for more CED studies in murine DIPG models.

SummaryThis paper describes the metabolic adaptation of Lactococcus lactis during the transition from a growing to a non‐growing state using retentostat cultivation. Under retentostat cultivation, the specific growth rate decreased from 0.025 h−1 to 0.0001 h−1 in 42 days, while doubling time increased to more than 260 days. Viability of the overall culture was maintained above 90% but included approximately 20% damaged cells, which had lost their colony forming capacity on solid media. Although culture biomass and viability had reached a steady‐state after 14 days of retentostat cultivation, the morphology of the cells changed from coccus‐to‐rod shape at later stages of retentostat cultivation, by which the cell's surface to volume ratio was estimated to increase 2.4‐fold. Furthermore, the metabolic patterns switched between homolactic and mixed‐acid fermentation during the retentostat cultivation. Retentostat cultivation enabled the calculation of accurate substrate‐ and energy‐related maintenance coefficients and biomass yields under non‐growing conditions, which were in good agreement with those calculated by extrapolation from chemostat cultivations at high dilution rates. In this study, we illustrate how retentostat cultivation allows decoupling of growth and non‐growth associated processes in L. lactis, enabling the analysis of quantitative physiological responses of this bacterium to near zero‐specific growth rates.

Wood biomass is turned into industrial fuel through chipping. The efficiency of chipping depends on many factors, including chipper knife wear. Chipper knife wear was determined through a long-term follow-up study, conducted at a waste wood recycling yard. Knife wear determined a sharp drop of productivity (>20%) and a severe decay in product quality. Dry sharpening with a grinder mitigated this effect, but it could not replace proper wet sharpening. Increasing the frequency of wet sharpening sessions determined a moderate increase of knife depreciation cost, but it could drastically enhance machine performance and reduce biomass processing cost. Since benefits largely exceed costs, increasing the frequency of wet sharpening sessions may be an effective measure for reducing overall chipping cost. If the main goal of a chipper operator is to increase productivity and/or decrease fuel consumption, then managing knife wear should be a primary target. (C) 2014 Elsevier Ltd. All rights reserved.

The drought prone North-West Bangladesh is vulnerable to the impacts of climate change, particularly because of less water availability in the dry period and high water requirement for crop production. Improved understanding of recent changes in crop water demand in the dry season is important for the water resources management in the region. A study was carried out to determine the potential impacts of recent climate change during last three decades on trends of water requirements of Boro rice. The reference crop evapotranspiration (ETo), potential crop water requirement (∑ETC), effective rainfall during the crop growing period (ER), potential irrigation requirement for crop evapotranspiration (∑ETC − ER) and net irrigation requirement of Boro rice were estimated using observed daily climate data in the CropWat model for the period of 1980 to 2013 for four North-West districts. Significant decreasing trends of ETo were observed in most of the dry months due to increasing relative humidity and decreasing wind-speed and sun-shine hours. The results showed decreasing trends of potential crop water requirement, i.e. the total crop evapotranspiration (∑ETC), of Boro rice due to decreasing reference crop evapotranspiration and shorter crop growing periods. The variations in trends of potential irrigation requirement for crop evapotranspiration (∑ETC − ER) found among different districts, are mainly linked to variations in trends of changes in effective rainfall. The net irrigation requirement of Boro rice has decreased, by 11% during the last three decades at an average rate of −4.4 mm year−1, instead of decreasing effective rainfall, mainly because of high rate of decrease of crop evapotranspiration (−5.9 mm year−1). Results indicate that a warming climate does not always result in higher agricultural water use and that climate change can also result in reduced water demands because of changes in humidity, wind-speed and sun-shine hours.