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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Mittal, Radhika; Brüggemann, Michael;

    Working Paper Series "Sustainable Lives - Food Choices" Project website This Working Paper was presented at the 69th Annual International Communication Association (ICA) Conference 2019 in Washington, D.C.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ https://dx.doi.org/1...arrow_drop_down
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    https://dx.doi.org/10.25592/uh...
    Research . 2019
    License: CC BY
    Data sources: Datacite
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    https://dx.doi.org/10.25592/uh...
    Research . 2019
    License: CC BY
    Data sources: Datacite
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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    Research . 2019
    Data sources: B2FIND
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ https://dx.doi.org/1...arrow_drop_down
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      https://dx.doi.org/10.25592/uh...
      Research . 2019
      License: CC BY
      Data sources: Datacite
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      https://dx.doi.org/10.25592/uh...
      Research . 2019
      License: CC BY
      Data sources: Datacite
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      B2FIND
      Research . 2019
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Pütz, Thomas; Wollschläger, Ute; Groh, Jannis; Gerke, Horst; +4 Authors

    The main goal of TERENO was to create observation platforms on the basis of an interdisciplinary and long‐term aimed research program. By the aid of SoilCan‐lysimeters, an ideal network was built between the four different TERENO‐observatories with their highly instrumented test sites. Within the four TERENO‐observatories at 13 different experimental sites, in total 126 lysimeters were filled monolithically and the measurements were started in October 2010. The lysimeters have been cultivated as grassland or arable land with a standardized crop rotation: winter wheat – winter barley – winter rye – oat.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ DLR publication serv...arrow_drop_down
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    Conference object . 2014
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    DLR publication server
    Other literature type . 2014
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ DLR publication serv...arrow_drop_down
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      DLR publication server
      Conference object . 2014
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      DLR publication server
      Other literature type . 2014
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Schröder, P.;

    For an enhancement in the time-space-yield of one-stage agricultural biogas plants, experiments for developing early warning indicators (EWI) for process failures and countermeasures for process stabilization and for adapting the substrate composition were conducted. A new EWI was developed, the A/elCon, the proportion of the concentration of organic acids to the electrical conductivity, which warned most effectively in lab- and full-scale experiments. As a countermeasure against process failures, the addition of different additives was tested. In general, the addition of additives counteracted over-acidifications at high organic loading rates (OLR). In contrast to increased OLRs without countermeasures, the microbial communities adapted to high OLRs, if additives were added, by an increase in the relative abundance of hydrogenotrophic and acid tolerant mixotrophic methanogens. The combination of trace elements and NaOH was most beneficial for stabilizing agricultural biogas processes and was successfully used in combination with the A/elCon to maintain a long-term high-performance digestion (up to OLR = 7.5 kg VS m 3 d 1, t > 100 d). A further increase in the time-space-yield was achieved by co-digesting energy-rich lipid-containing substrates. As known from the digestion of biological waste, the high-performance co-digestions were stabilized by CaO additions leading to an aggregate formation. The stabilization was based on the precipitation of toxic long-chain fatty acids with calcium and thus the formation of surfaces for biofilms. Even a long-term high-performance co-digestion (up to OLR = 9 kg VS m 3 d 1, t > 100 d) was established by adding CaO. In this connection the formation of highly effective fatty acid degrading microbial consortia was detected consisting of syntrophic bacteria and hydrogenotrophic archaea that contributed to the process stability. Analyzing the microbial communities the results of the metagenome analysis and of the classical genetic fingerprinting via DGGE were compared. Both methods showed an increase in acidogenic bacteria and thereby especially in lactic acid producing bacteria with increasing loading rates. The results of the metagenome analysis were much more detailed. Zur Steigerung der Raum-Zeit-Ausbeute in einstufigen, landwirtschaftlichen Biogasanlagen wurden Versuche zur Entwicklung von Frühwarnindikatoren (FWI) für Prozessstörungen und von Maßnahmen zur Prozessstabilisierung, sowie zur Substratzusammensetzung durchgeführt. Ein neuer FWI wurde entwickelt, der A/elCon, das Verhältnis aus der Konzentration organischer Säuren (engl.: org. acids) zur elektrischen Leitfähigkeit (engl.: electr. conductivity), der im Labor und in der Großtechnik am effektivsten vor drohenden Prozessstörungen warnte. Als Maßnahme zur Prozessstabilisierung wurde die Zugabe von Additiven untersucht. Grundsätzlich wurde durch die Additivzugabe eine Übersäuerung bei hohen Raumbelastungen vermieden. Im Unterschied zur Leistungssteigerung ohne Additivzugabe, passte sich die mikrobielle Lebensgemeinschaft durch Zunahme der relativen Abundanz hydrogenotropher und säuretoleranter mixotropher Methanogener an hohe Raumbelastungen (BR) an. Eine Kombination aus Spurenelementen und NaOH eignete sich am besten zur Prozessstabilisierung und wurde zusammen mit dem A/elCon erfolgreich zur Etablierung einer Langzeit-Hochlast-Vergärung (bis BR = 7,5 kg oTS m 3 d 1, t > 100 d) von NawaRo eingesetzt. Eine weitere Leistungssteigerung wurde durch Co-Vergärung energiereicher lipidhaltiger Substrate erzielt. Wie aus Anlagen der Abfallwirtschaft bekannt, wurden die Hochlast-Co-Vergärungen bei CaO-Zugabe durch eine Aggregatbildung stabilisiert. Die Aggregate binden toxische, langkettige Fettsäuren und bilden Oberflächen für Biofilme. Es wurde auch eine Langzeit-Hochlast-Co-Vergärung (t > 100 d) durch Zugabe von CaO etabliert. Dabei wurde die Ausbildung einer hocheffektiven fettsäureabbauenden mikrobiellen Lebensgemeinschaft, bestehend aus syntrophen Bacteria und hydrogenotrophen Archaea detektiert, die entscheidend zur Prozessstabilität beitrug. Bei der Charakterisierung der mikrobiellen Biozönosen wurden die Ergebnisse der Metagenomanalyse und des klassischen genetischen Fingerprintings mittels DGGE verglichen. Beide Methoden zeigten eine Zunahme acidogener Bacteria und dabei speziell von Milchsäurebakterien mit zunehmender Raumbelastung. Die Ergebnisse der Metagenomanalyse waren deutlich detaillierter.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ https://dx.doi.org/1...arrow_drop_down
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    https://dx.doi.org/10.14279/de...
    Doctoral thesis . 2018
    License: CC BY
    Data sources: Datacite
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    Doctoral thesis . 2018
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      Doctoral thesis . 2018
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  • Authors: Heißenhuber, Alois; Zehetmeier, Monika;

    The increase in milk yield per cow in dairy farms has been proposed as one strategy to reduce greenhouse gas emissions (GHG) per kg of product. However, besides milk beef can be considered as an important co-product of dairy farming. Thus, the whole system of milk and beef production has to be considered modelling GHG emissions from dairy farming. In order to remain milk and beef production constant suckler cows are needed to compensate for the reduction in beef output from dairy farming due to the increase in milk yield. If both milk and beef production are to remain constant, considerably increasing milk yield per cow could result in higher GHG emissions. Furthermore other side effects of increasing milk yield in dairy farming as competition with human food due to changing amounts of concentrates need to be mentioned. Für die Minderung der Treibhausgasemission je Produkteinheit wird die Leistungssteigerung in der Milchviehhaltung als eine wirksame Maßnahme betrachtet. Da in der Milchviehhaltung üblicherweise Milch und Fleisch erzeugt wird, ist eine Betrachtungsweise des Gesamtsystems erforderlich. Unter der Annahme einer konstanten Produktion an Milch und Rindfleisch, werden mit steigernder Milchleistung zusätzlich Mutterkühe zur Bereitstellung von Rindfleisch benötigt. Aufgrund dieser Zusammenhänge führt eine Steigerung der Milchleistung zu einem tendenziellen Anstieg der THG-Emissionen. Darüber hinaus sind noch Nebenwirkungen zu berücksichtigen, wie z.B. der Aspekt der Nahrungskonkurrenz bei unterschiedlichem Kraftfuttereinsatz. Jahrbuch Agrartechnik, vol. 24. 2012Jahrbuch Agrartechnik 2012, vol. 24.2012, p. 3

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    Die Akzeptanz kleinräumiger Angebote der Lebensmittelversorgung im Untersuchungsraum Stuttgart steht im Mittelpunkt dieses Arbeitsberichts, der grundsätzliche Überlegungen und aktuelle empirische Untersuchungen am Fachgebiet Agrarmärkte und Agrarmarketing der Universität Hohenheim hierzu bündelt. Betrachtet werden die Akteure Verbraucher und Unternehmen in Stuttgart gleichermaßen. Die zentralen Fragen der Untersuchungen lauten: Was bedeuten einer Stadtbevölkerung Lebensmittel regionaler Herkunft? Wie werden kleinräumige Angebote der Lebensmittelversorgung von den urbanen Verbrauchern im Untersuchungsraum Stuttgart angenommen und welche Bedeutung können diese in Zukunft gewinnen? Welche Bedeutung haben Lebensmittel regionaler Herkunft für Händler und Direktvermarkter in Stuttgart und welche Entwicklungen lassen sich ableiten? The working paper analyzes the acceptance of short-chain food supply systems by consumers and companies in Stuttgart, the provincial capital of Baden-Württemberg, based on theoretical considerations and empirical studies carried out by the Chair of Agricultural Markets and Marketing at the University of Hohenheim. The key questions of the empirical studies focus on the following points: How does the city population reflect the importance of regional foods? What are the attitudes and purchase behavior of urban consumers in regard to regional food or self production and what importance can the subjects gain in the future? Of which importance is regional food to local merchants? assortment in Stuttgart and which future developments can we derive?

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  • Authors: Mellmann, Jochen; Weigler, Fabian; Hoffmann, Thomas;

    Betriebseigene Getreideanlagen sind für die Mehrzahl der Erzeuger nach wie vor unverzichtbar. Eine zunehmende Überschneidung der Erntefenster, steigende Mähdreschkapazitäten und daraus resultierende Anforderungen an die Logistik zwingen viele Betriebe zum Neubau bzw. zur Erweiterung ihrer Anlagen. Die Steigerung der Energieeffizienz und Senkung der Verfahrenskosten stehen im Fokus aktueller Forschung und Entwicklung. In-house grain plants are still indispensable for the majority of producers. Due to a growing overlap of harvest times, increasing capacity of the harvesters and the resulting requirements for the logistic, more and more farmers are forced to build new facilities or extend existing plants. The actual research and development is focused on increasing energy efficiency and lowering process costs. Jahrbuch Agrartechnik, vol. 26. 2014Jahrbuch Agrartechnik 2014, vol. 26.2014, p. 171

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    Als Beitrag zur Sicherung der Welternährung fördert das Bundesministerium für Bildung und Forschung (BMBF) unter Beteiligung des Bundesministeriums für wirtschaftliche Zusammenarbeit und Entwicklung (BMZ) seit 2013 mehrere Verbundprojekte, an welchen das Fachgebiet für Agrartechnik der Universität Kassel und das Fachgebiet Agrartechnik in den Tropen und Subtropen der Universität Hohenheim mit Forschungsarbeiten zur Verbesserung der Nutzung von Grundnahrungsmitteln in Afrika beteiligt sind. Ein Schwerpunkt liegt dabei auf der Verbesserung der Nacherntetechnologie zur Vermeidung von Verlusten sowie zur Wertsteigerung durch Weiterverarbeitung. Since 2013, the Federal Ministry of Education and Research (BMBF) and the Federal Ministry of Economic Cooperation and Development (BMZ) is funding several joint research projects to contribute to food security. Agricultural and Biosystems Engineering Group of the University of Kassel and Agricultural Engineering Tropics and Subtropics Group of the University of Hohenheim are contributing to those projects to improve the utilization of staple crops in Africa. Focus is on improvement of postharvest technology to reduce food losses and to add value by processing. Jahrbuch Agrartechnik, vol. 29Jahrbuch Agrartechnik 2017, vol. 29, 2017

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    https://dx.doi.org/10.24355/db...
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  • Authors: Griepentrog, Hans W.; Volz, Frank;

    Das Prüfwesen in der Landtechnik soll eine hohe Qualität der Maschinen und Geräte sicherstellen. Über die dynamische Entwicklung der Messtechnik erhalten Prüfverfahren neue Impulse oder es werden heute sogar neue Verfahren ermöglicht, die bisher nicht bekannt oder zu aufwendig waren. Im Mittelpunkt steht heute häufig immer noch der Traktor, dessen Verbrauchswerte mittels Prüfverfahren realistisch dargestellt werden. Hohe Investitionen für eine Verbesserung dieser Prüfverfahren werden in Zukunft noch zuverlässigere Informationen sicherstellen. Aus der Forschung kommen neue Verfahren der Sensorik und Datenanalyse, um komplexe Strukturen beschreiben und extrahieren zu können, die beispielsweise eine Vereinfachung der Bewertung der Arbeitsqualität von Bodenbearbeitungsgeräten erlauben. Test engineering in agricultural machinery ensures high quality in all machinery components. Latest instrumentation contributes to improve test procedures or creates new opportunities that were not known or possible. Today the tractor still is in focus, but its fuel consumption is well documented due to realistic test procedures. High investments in those procedures today make sure that in the future this information will be even more reliable. Research institutions investigated sensors and data algorithms to describe and extract complex structures being able to simplify the assessment of the work quality of tillage implements. Jahrbuch Agrartechnik, vol. 26. 2014Jahrbuch Agrartechnik 2014, vol. 26.2014, p. 227

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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/

    Sustainability awards are an exclusive instrument of sustainability communication. They are significant limited - mainly quantitative. Not everyone can get such a price. Often only a few, mostly one, wins a contest.Interest in sustainability prices grows. This interest express companies, the potential winners,and show the donors of awards, the awarding institutions. In the last yearsmore and more sustainability competitions in Germanyare founded.Meanwhile, companies in the food industry can participatein 24 competitions with 82 categories (as of 2013). It is a dynamic market of sustainability awards - with lots of potential, but also some challenges. Independence and transparency of procurement become important elements of awards’ concepts. In this report, 24 sustainability competitions and their categories are featured in factsheets. This include the relevant competitions directlyaimed at companies in the food industry or be open for those.The approach to identify them isdescribed. In addition, the evaluation scheme of transparency of sustainability competitions is explained. Key results are described. Nachhaltigkeitspreise, engl. Awards, sind ein exklusives Instrument der Nachhaltigkeits-kommunikation. Sie unterliegen hohen Zugangsbeschränkungen - vor allem quantitativen, die in den Händen der Vergabeinstitutionen und deren Juroren liegen. Nicht jeder kann einen solchen Preis erhalten. Oft werden nur wenige, teils nur ein Preisträger in einem Wettbewerb ausgewählt. Vor diesem Hintergrund wächst das Interesse an Nachhaltigkeitspreisen. Diese Interesse bekunden auchUnternehmen bzw. zeigen die Geber der Awards, die Vergabeinstitutionen: es werden in Deutschland in den letzten Jahren immer mehr Nachhaltigkeitswettbewerbe eingerichtet und viele Awards verliehen. Es ist ein dynamischer Markt der Nachhaltigkeitsawards entstanden – mit viel Potenzial, aber auch einigen Herausforderungen. Inzwischen können sich Unternehmen der Ernährungswirtschaft an 24 Wettbewerben in 82 Kategorien beteiligen (Stand: 2013). Unabhängigkeit und Transparenz der Vergabe werden zu wichtigen Gestaltungselementen von Awards, deren Etablierung und Bekanntheit zu Förderern ihrer Reputation. In diesem Arbeitsbericht werden 24 Nachhaltigkeitswettbewerbe und ihre Preiskategorien, die sich an Unternehmen der Ernährungswirtschaft richten bzw. für die geöffnet sind, in Fact Sheets vorgestellt. Die methodische Vorgehensweise der Identifizierung der relevanten Wettbewerbe und deren Beschreibung auf Basis einer Desktop-Recherche sind dokumentarisch vorangestellt. Außerdem wird das Bewertungsschema der Transparenz von Nachhaltigkeitswettbewerben aus Sicht eines normal Interessierten erläutert sowie zentrale Ergebnisse hierzu beschrieben.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ EconStorarrow_drop_down
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    Research . 2016
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    https://dx.doi.org/10.22004/ag...
    Other literature type . 2016
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    External research report . 2016
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ EconStorarrow_drop_down
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      Research . 2016
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      https://dx.doi.org/10.22004/ag...
      Other literature type . 2016
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      External research report . 2016
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Nettmann, Edith;

    Die Produktion von Biogas aus landwirtschaftlichen Primärprodukten oder Reststoffen stellt einen we-sentlichen Beitrag zur Reduktion des CO2-Ausstoßes sowie zur Entwicklung einer nachhaltigen Landbewirtschaftung dar. Gegenwärtig stehen im Fokus der Forschung die Entwicklung und Optimierung von Biogasreaktoren und Betriebstechniken sowie die Optimierung der Substratbereitstellung. Über die Zusammensetzung der an der Biogasbildung beteiligten mikrobiellen Lebensgemeinschaften in landwirt-schaftlichen Biogasanlagen gibt es jedoch bis heute nur wenige Informationen. Im Rahmen dieser Studie wurde die Struktur der methanogenen Biozönose in zehn landwirtschaftlichen Biogasanlagen, welche auf Basis von Nachwachsenden Rohstoffen (NawaRo) betrieben wurden, unter-sucht. Hierzu wurde ein polyphasischer Ansatz mit verschiedenen kultivierungsunabhängigen, molekularen Verfahren gewählt. Primär wurde eine PCR-RFLP Analyse der Nukleotidsequenz der 16S rDNA sowie des mcrA Gens durchgeführt. Die Bestimmung der relativen Häufigkeit der Methan bil-denden Mikroorganismen in den Proben erfolgte mittels quantitativer real-time PCR (Q-PCR) auf Basis gruppenspezifischer Primer für das 16S rDNA Gen. Für ausgewählte Biogasreaktoren wurde ergänzend eine mikroskopische Quantifizierung mittels Fluoreszenz in situ Hybridisierung (FISH) durchgeführt. Für die FISH wurden spezifische Sonden für die Domänen Bacteria (EUB338) und Archaea (ARCH915) so-wie für die Ordnungen Methanomicrobiales (MG1200), Methanobacteriales (MB311 und MB1174) und den Familien Methanosarcinaceae (Ms821) und Methanosaetaceae (Mx825) verwendet. Die Ergebnisse der Analysen zeigten, dass in neun der zehn untersuchten Biogasanlagen die hydroge-notrophen Methanogenen, repräsentiert durch Vertreter der Ordnungen Methanomicrobiales und Methanobacteriales, vorherrschend waren. In diesen Biogasreaktoren wurde die Gattung Methanoculleus (Ordnung Methanomicrobiales) als dominierende Gattung nachgewiesen. Nennenswerte Anteile an ace-toklastischen Methanbildnern, insbesondere der Gattung Methanosaeta, konnten für sechs der untersuchten zehn Anlagen nachgewiesen werden. Jedoch wurde nur in einer Biogasanlage diese Gattung als die dominierende Gruppe von Methanbildnern nachgewiesen. Diese Ergebnisse deuten darauf hin, dass in 90 % der untersuchten Biogasanlagen die hydrogenotrophe Methanogenese der vorrangige Stoff-wechselweg für die Methanproduktion ist. In dieser Studie konnte kein Zusammenhang zwischen den jeweils zur Biogasgewinnung eingesetzten Substraten und der Struktur der Archaea-Biozönose beobachtet werden. Ebenso wenig scheinen unter-schiedliche Verweilzeiten der Substrate sowie die Raumbelastung der Reaktoren einen Einfluss auf die methanogenen Archaea zu haben. Jedoch ließ sich ein negativer Einfluss hoher Ammonium- bzw. Am-moniakkonzentrationen auf das Wachstum acetoklastischer Methanbildner, insbesondere Methanosaeta spp., in den untersuchten landwirtschaftlichen Biogasanlagen feststellen. Die aus dieser Studie gewonnenen Erkenntnisse dienen dem grundlegenden Verständnis der methanoge-nen Lebensgemeinschaft in landwirtschaftlichen Biogasanlagen, und sollen somit zur weiteren Optimierung der Biogasgewinnung beitragen. Biogas production from agricultural main products or remnants provides a substantial contribution to the reduction of CO2 emissions but also to the development of a sustainable agriculture. Currently, the re-search focuses on development and optimization of biogas reactors and reactor performance as well as optimization of substrate eligibility. However, the knowledge of the structure and dynamics of microbial communities which are involved in the biogas production is still insufficient. Within this study, the structures of methanogenic biocoenosis in ten agricultural biogas plants which are operated on basis of renewable raw materials were analyzed. Therefore a polyphasic approach with differ-ent culture independent molecular genetic techniques was chosen. First, PCR-RFLP analyses of 16S rDNA and mcrA genes were conducted. For determination of the relative abundance of methane pro-ducing microorganisms in the samples a quantitative real-time PCR (Q-PCR) on basis of 16S rDNA group-specific primers was applied. Fluorescence in situ hybridization (FISH) was carried out for direct quantification of methanogenic cells in reactor contents of six of the ten biogas plants. FISH was carried out with specific probes for the domains Bacteria (EUB338) and Archaea (ARCH915), the orders Metha-nomicrobiales (MG1200) and Methanobacteriales (MB311 and MB1174) and the families Methanosarcinaceae (Ms821) and Methanosaetaceae (Mx825). The results of the analyses showed, that in nine of the ten biogas plants the hydrogenotrophic methano-gens, represented by the orders Methanomicrobiales and Methanobacteriales, were prevalent. The genus Methanoculleus (Methanomicrobiales) was the predominating genus in these biogas plants. Acetoclastic methanogens such as organisms of the genus Methanosaeta were found only in six of the ten analyzed biogas plants. However, in one biogas plant Methanosaeta was determined as the predominant methano-genic group. These results indicate that the hydrogenotrophic methanogenesis was the major metabolic pathway for methane production in 90 % of the analyzed biogas plants. In this study, no correlation between used substrates for biogas production and the structure of Archaea biocoenosis could be observed. Also, the retention time of substrates and the organic loading rate of reac-tors seemed to have no obvious effect on the methanogenic Archaea. However, in the analyzed biogas plants a negative influence of high ammonium and ammonia concentrations, respectively, on the growth of acetoclastic methanogens in particular Methanosaeta spp. was determined. The results of this study serve the basic understanding of the methanogen community in agricultural bio-gas plants and are to contribute to further optimization of biogas production.

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    Doctoral thesis . 2010
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    https://dx.doi.org/10.14279/de...
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Mittal, Radhika; Brüggemann, Michael;

    Working Paper Series "Sustainable Lives - Food Choices" Project website This Working Paper was presented at the 69th Annual International Communication Association (ICA) Conference 2019 in Washington, D.C.

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    https://dx.doi.org/10.25592/uh...
    Research . 2019
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    https://dx.doi.org/10.25592/uh...
    Research . 2019
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    Research . 2019
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      Research . 2019
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      Research . 2019
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      Research . 2019
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    Authors: Pütz, Thomas; Wollschläger, Ute; Groh, Jannis; Gerke, Horst; +4 Authors

    The main goal of TERENO was to create observation platforms on the basis of an interdisciplinary and long‐term aimed research program. By the aid of SoilCan‐lysimeters, an ideal network was built between the four different TERENO‐observatories with their highly instrumented test sites. Within the four TERENO‐observatories at 13 different experimental sites, in total 126 lysimeters were filled monolithically and the measurements were started in October 2010. The lysimeters have been cultivated as grassland or arable land with a standardized crop rotation: winter wheat – winter barley – winter rye – oat.

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    Authors: Schröder, P.;

    For an enhancement in the time-space-yield of one-stage agricultural biogas plants, experiments for developing early warning indicators (EWI) for process failures and countermeasures for process stabilization and for adapting the substrate composition were conducted. A new EWI was developed, the A/elCon, the proportion of the concentration of organic acids to the electrical conductivity, which warned most effectively in lab- and full-scale experiments. As a countermeasure against process failures, the addition of different additives was tested. In general, the addition of additives counteracted over-acidifications at high organic loading rates (OLR). In contrast to increased OLRs without countermeasures, the microbial communities adapted to high OLRs, if additives were added, by an increase in the relative abundance of hydrogenotrophic and acid tolerant mixotrophic methanogens. The combination of trace elements and NaOH was most beneficial for stabilizing agricultural biogas processes and was successfully used in combination with the A/elCon to maintain a long-term high-performance digestion (up to OLR = 7.5 kg VS m 3 d 1, t > 100 d). A further increase in the time-space-yield was achieved by co-digesting energy-rich lipid-containing substrates. As known from the digestion of biological waste, the high-performance co-digestions were stabilized by CaO additions leading to an aggregate formation. The stabilization was based on the precipitation of toxic long-chain fatty acids with calcium and thus the formation of surfaces for biofilms. Even a long-term high-performance co-digestion (up to OLR = 9 kg VS m 3 d 1, t > 100 d) was established by adding CaO. In this connection the formation of highly effective fatty acid degrading microbial consortia was detected consisting of syntrophic bacteria and hydrogenotrophic archaea that contributed to the process stability. Analyzing the microbial communities the results of the metagenome analysis and of the classical genetic fingerprinting via DGGE were compared. Both methods showed an increase in acidogenic bacteria and thereby especially in lactic acid producing bacteria with increasing loading rates. The results of the metagenome analysis were much more detailed. Zur Steigerung der Raum-Zeit-Ausbeute in einstufigen, landwirtschaftlichen Biogasanlagen wurden Versuche zur Entwicklung von Frühwarnindikatoren (FWI) für Prozessstörungen und von Maßnahmen zur Prozessstabilisierung, sowie zur Substratzusammensetzung durchgeführt. Ein neuer FWI wurde entwickelt, der A/elCon, das Verhältnis aus der Konzentration organischer Säuren (engl.: org. acids) zur elektrischen Leitfähigkeit (engl.: electr. conductivity), der im Labor und in der Großtechnik am effektivsten vor drohenden Prozessstörungen warnte. Als Maßnahme zur Prozessstabilisierung wurde die Zugabe von Additiven untersucht. Grundsätzlich wurde durch die Additivzugabe eine Übersäuerung bei hohen Raumbelastungen vermieden. Im Unterschied zur Leistungssteigerung ohne Additivzugabe, passte sich die mikrobielle Lebensgemeinschaft durch Zunahme der relativen Abundanz hydrogenotropher und säuretoleranter mixotropher Methanogener an hohe Raumbelastungen (BR) an. Eine Kombination aus Spurenelementen und NaOH eignete sich am besten zur Prozessstabilisierung und wurde zusammen mit dem A/elCon erfolgreich zur Etablierung einer Langzeit-Hochlast-Vergärung (bis BR = 7,5 kg oTS m 3 d 1, t > 100 d) von NawaRo eingesetzt. Eine weitere Leistungssteigerung wurde durch Co-Vergärung energiereicher lipidhaltiger Substrate erzielt. Wie aus Anlagen der Abfallwirtschaft bekannt, wurden die Hochlast-Co-Vergärungen bei CaO-Zugabe durch eine Aggregatbildung stabilisiert. Die Aggregate binden toxische, langkettige Fettsäuren und bilden Oberflächen für Biofilme. Es wurde auch eine Langzeit-Hochlast-Co-Vergärung (t > 100 d) durch Zugabe von CaO etabliert. Dabei wurde die Ausbildung einer hocheffektiven fettsäureabbauenden mikrobiellen Lebensgemeinschaft, bestehend aus syntrophen Bacteria und hydrogenotrophen Archaea detektiert, die entscheidend zur Prozessstabilität beitrug. Bei der Charakterisierung der mikrobiellen Biozönosen wurden die Ergebnisse der Metagenomanalyse und des klassischen genetischen Fingerprintings mittels DGGE verglichen. Beide Methoden zeigten eine Zunahme acidogener Bacteria und dabei speziell von Milchsäurebakterien mit zunehmender Raumbelastung. Die Ergebnisse der Metagenomanalyse waren deutlich detaillierter.

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    https://dx.doi.org/10.14279/de...
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  • Authors: Heißenhuber, Alois; Zehetmeier, Monika;

    The increase in milk yield per cow in dairy farms has been proposed as one strategy to reduce greenhouse gas emissions (GHG) per kg of product. However, besides milk beef can be considered as an important co-product of dairy farming. Thus, the whole system of milk and beef production has to be considered modelling GHG emissions from dairy farming. In order to remain milk and beef production constant suckler cows are needed to compensate for the reduction in beef output from dairy farming due to the increase in milk yield. If both milk and beef production are to remain constant, considerably increasing milk yield per cow could result in higher GHG emissions. Furthermore other side effects of increasing milk yield in dairy farming as competition with human food due to changing amounts of concentrates need to be mentioned. Für die Minderung der Treibhausgasemission je Produkteinheit wird die Leistungssteigerung in der Milchviehhaltung als eine wirksame Maßnahme betrachtet. Da in der Milchviehhaltung üblicherweise Milch und Fleisch erzeugt wird, ist eine Betrachtungsweise des Gesamtsystems erforderlich. Unter der Annahme einer konstanten Produktion an Milch und Rindfleisch, werden mit steigernder Milchleistung zusätzlich Mutterkühe zur Bereitstellung von Rindfleisch benötigt. Aufgrund dieser Zusammenhänge führt eine Steigerung der Milchleistung zu einem tendenziellen Anstieg der THG-Emissionen. Darüber hinaus sind noch Nebenwirkungen zu berücksichtigen, wie z.B. der Aspekt der Nahrungskonkurrenz bei unterschiedlichem Kraftfuttereinsatz. Jahrbuch Agrartechnik, vol. 24. 2012Jahrbuch Agrartechnik 2012, vol. 24.2012, p. 3

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    Die Akzeptanz kleinräumiger Angebote der Lebensmittelversorgung im Untersuchungsraum Stuttgart steht im Mittelpunkt dieses Arbeitsberichts, der grundsätzliche Überlegungen und aktuelle empirische Untersuchungen am Fachgebiet Agrarmärkte und Agrarmarketing der Universität Hohenheim hierzu bündelt. Betrachtet werden die Akteure Verbraucher und Unternehmen in Stuttgart gleichermaßen. Die zentralen Fragen der Untersuchungen lauten: Was bedeuten einer Stadtbevölkerung Lebensmittel regionaler Herkunft? Wie werden kleinräumige Angebote der Lebensmittelversorgung von den urbanen Verbrauchern im Untersuchungsraum Stuttgart angenommen und welche Bedeutung können diese in Zukunft gewinnen? Welche Bedeutung haben Lebensmittel regionaler Herkunft für Händler und Direktvermarkter in Stuttgart und welche Entwicklungen lassen sich ableiten? The working paper analyzes the acceptance of short-chain food supply systems by consumers and companies in Stuttgart, the provincial capital of Baden-Württemberg, based on theoretical considerations and empirical studies carried out by the Chair of Agricultural Markets and Marketing at the University of Hohenheim. The key questions of the empirical studies focus on the following points: How does the city population reflect the importance of regional foods? What are the attitudes and purchase behavior of urban consumers in regard to regional food or self production and what importance can the subjects gain in the future? Of which importance is regional food to local merchants? assortment in Stuttgart and which future developments can we derive?

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  • Authors: Mellmann, Jochen; Weigler, Fabian; Hoffmann, Thomas;

    Betriebseigene Getreideanlagen sind für die Mehrzahl der Erzeuger nach wie vor unverzichtbar. Eine zunehmende Überschneidung der Erntefenster, steigende Mähdreschkapazitäten und daraus resultierende Anforderungen an die Logistik zwingen viele Betriebe zum Neubau bzw. zur Erweiterung ihrer Anlagen. Die Steigerung der Energieeffizienz und Senkung der Verfahrenskosten stehen im Fokus aktueller Forschung und Entwicklung. In-house grain plants are still indispensable for the majority of producers. Due to a growing overlap of harvest times, increasing capacity of the harvesters and the resulting requirements for the logistic, more and more farmers are forced to build new facilities or extend existing plants. The actual research and development is focused on increasing energy efficiency and lowering process costs. Jahrbuch Agrartechnik, vol. 26. 2014Jahrbuch Agrartechnik 2014, vol. 26.2014, p. 171

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    Als Beitrag zur Sicherung der Welternährung fördert das Bundesministerium für Bildung und Forschung (BMBF) unter Beteiligung des Bundesministeriums für wirtschaftliche Zusammenarbeit und Entwicklung (BMZ) seit 2013 mehrere Verbundprojekte, an welchen das Fachgebiet für Agrartechnik der Universität Kassel und das Fachgebiet Agrartechnik in den Tropen und Subtropen der Universität Hohenheim mit Forschungsarbeiten zur Verbesserung der Nutzung von Grundnahrungsmitteln in Afrika beteiligt sind. Ein Schwerpunkt liegt dabei auf der Verbesserung der Nacherntetechnologie zur Vermeidung von Verlusten sowie zur Wertsteigerung durch Weiterverarbeitung. Since 2013, the Federal Ministry of Education and Research (BMBF) and the Federal Ministry of Economic Cooperation and Development (BMZ) is funding several joint research projects to contribute to food security. Agricultural and Biosystems Engineering Group of the University of Kassel and Agricultural Engineering Tropics and Subtropics Group of the University of Hohenheim are contributing to those projects to improve the utilization of staple crops in Africa. Focus is on improvement of postharvest technology to reduce food losses and to add value by processing. Jahrbuch Agrartechnik, vol. 29Jahrbuch Agrartechnik 2017, vol. 29, 2017

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    https://dx.doi.org/10.24355/db...
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    Article . 2020
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  • Authors: Griepentrog, Hans W.; Volz, Frank;

    Das Prüfwesen in der Landtechnik soll eine hohe Qualität der Maschinen und Geräte sicherstellen. Über die dynamische Entwicklung der Messtechnik erhalten Prüfverfahren neue Impulse oder es werden heute sogar neue Verfahren ermöglicht, die bisher nicht bekannt oder zu aufwendig waren. Im Mittelpunkt steht heute häufig immer noch der Traktor, dessen Verbrauchswerte mittels Prüfverfahren realistisch dargestellt werden. Hohe Investitionen für eine Verbesserung dieser Prüfverfahren werden in Zukunft noch zuverlässigere Informationen sicherstellen. Aus der Forschung kommen neue Verfahren der Sensorik und Datenanalyse, um komplexe Strukturen beschreiben und extrahieren zu können, die beispielsweise eine Vereinfachung der Bewertung der Arbeitsqualität von Bodenbearbeitungsgeräten erlauben. Test engineering in agricultural machinery ensures high quality in all machinery components. Latest instrumentation contributes to improve test procedures or creates new opportunities that were not known or possible. Today the tractor still is in focus, but its fuel consumption is well documented due to realistic test procedures. High investments in those procedures today make sure that in the future this information will be even more reliable. Research institutions investigated sensors and data algorithms to describe and extract complex structures being able to simplify the assessment of the work quality of tillage implements. Jahrbuch Agrartechnik, vol. 26. 2014Jahrbuch Agrartechnik 2014, vol. 26.2014, p. 227

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    Sustainability awards are an exclusive instrument of sustainability communication. They are significant limited - mainly quantitative. Not everyone can get such a price. Often only a few, mostly one, wins a contest.Interest in sustainability prices grows. This interest express companies, the potential winners,and show the donors of awards, the awarding institutions. In the last yearsmore and more sustainability competitions in Germanyare founded.Meanwhile, companies in the food industry can participatein 24 competitions with 82 categories (as of 2013). It is a dynamic market of sustainability awards - with lots of potential, but also some challenges. Independence and transparency of procurement become important elements of awards’ concepts. In this report, 24 sustainability competitions and their categories are featured in factsheets. This include the relevant competitions directlyaimed at companies in the food industry or be open for those.The approach to identify them isdescribed. In addition, the evaluation scheme of transparency of sustainability competitions is explained. Key results are described. Nachhaltigkeitspreise, engl. Awards, sind ein exklusives Instrument der Nachhaltigkeits-kommunikation. Sie unterliegen hohen Zugangsbeschränkungen - vor allem quantitativen, die in den Händen der Vergabeinstitutionen und deren Juroren liegen. Nicht jeder kann einen solchen Preis erhalten. Oft werden nur wenige, teils nur ein Preisträger in einem Wettbewerb ausgewählt. Vor diesem Hintergrund wächst das Interesse an Nachhaltigkeitspreisen. Diese Interesse bekunden auchUnternehmen bzw. zeigen die Geber der Awards, die Vergabeinstitutionen: es werden in Deutschland in den letzten Jahren immer mehr Nachhaltigkeitswettbewerbe eingerichtet und viele Awards verliehen. Es ist ein dynamischer Markt der Nachhaltigkeitsawards entstanden – mit viel Potenzial, aber auch einigen Herausforderungen. Inzwischen können sich Unternehmen der Ernährungswirtschaft an 24 Wettbewerben in 82 Kategorien beteiligen (Stand: 2013). Unabhängigkeit und Transparenz der Vergabe werden zu wichtigen Gestaltungselementen von Awards, deren Etablierung und Bekanntheit zu Förderern ihrer Reputation. In diesem Arbeitsbericht werden 24 Nachhaltigkeitswettbewerbe und ihre Preiskategorien, die sich an Unternehmen der Ernährungswirtschaft richten bzw. für die geöffnet sind, in Fact Sheets vorgestellt. Die methodische Vorgehensweise der Identifizierung der relevanten Wettbewerbe und deren Beschreibung auf Basis einer Desktop-Recherche sind dokumentarisch vorangestellt. Außerdem wird das Bewertungsschema der Transparenz von Nachhaltigkeitswettbewerben aus Sicht eines normal Interessierten erläutert sowie zentrale Ergebnisse hierzu beschrieben.

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    Authors: Nettmann, Edith;

    Die Produktion von Biogas aus landwirtschaftlichen Primärprodukten oder Reststoffen stellt einen we-sentlichen Beitrag zur Reduktion des CO2-Ausstoßes sowie zur Entwicklung einer nachhaltigen Landbewirtschaftung dar. Gegenwärtig stehen im Fokus der Forschung die Entwicklung und Optimierung von Biogasreaktoren und Betriebstechniken sowie die Optimierung der Substratbereitstellung. Über die Zusammensetzung der an der Biogasbildung beteiligten mikrobiellen Lebensgemeinschaften in landwirt-schaftlichen Biogasanlagen gibt es jedoch bis heute nur wenige Informationen. Im Rahmen dieser Studie wurde die Struktur der methanogenen Biozönose in zehn landwirtschaftlichen Biogasanlagen, welche auf Basis von Nachwachsenden Rohstoffen (NawaRo) betrieben wurden, unter-sucht. Hierzu wurde ein polyphasischer Ansatz mit verschiedenen kultivierungsunabhängigen, molekularen Verfahren gewählt. Primär wurde eine PCR-RFLP Analyse der Nukleotidsequenz der 16S rDNA sowie des mcrA Gens durchgeführt. Die Bestimmung der relativen Häufigkeit der Methan bil-denden Mikroorganismen in den Proben erfolgte mittels quantitativer real-time PCR (Q-PCR) auf Basis gruppenspezifischer Primer für das 16S rDNA Gen. Für ausgewählte Biogasreaktoren wurde ergänzend eine mikroskopische Quantifizierung mittels Fluoreszenz in situ Hybridisierung (FISH) durchgeführt. Für die FISH wurden spezifische Sonden für die Domänen Bacteria (EUB338) und Archaea (ARCH915) so-wie für die Ordnungen Methanomicrobiales (MG1200), Methanobacteriales (MB311 und MB1174) und den Familien Methanosarcinaceae (Ms821) und Methanosaetaceae (Mx825) verwendet. Die Ergebnisse der Analysen zeigten, dass in neun der zehn untersuchten Biogasanlagen die hydroge-notrophen Methanogenen, repräsentiert durch Vertreter der Ordnungen Methanomicrobiales und Methanobacteriales, vorherrschend waren. In diesen Biogasreaktoren wurde die Gattung Methanoculleus (Ordnung Methanomicrobiales) als dominierende Gattung nachgewiesen. Nennenswerte Anteile an ace-toklastischen Methanbildnern, insbesondere der Gattung Methanosaeta, konnten für sechs der untersuchten zehn Anlagen nachgewiesen werden. Jedoch wurde nur in einer Biogasanlage diese Gattung als die dominierende Gruppe von Methanbildnern nachgewiesen. Diese Ergebnisse deuten darauf hin, dass in 90 % der untersuchten Biogasanlagen die hydrogenotrophe Methanogenese der vorrangige Stoff-wechselweg für die Methanproduktion ist. In dieser Studie konnte kein Zusammenhang zwischen den jeweils zur Biogasgewinnung eingesetzten Substraten und der Struktur der Archaea-Biozönose beobachtet werden. Ebenso wenig scheinen unter-schiedliche Verweilzeiten der Substrate sowie die Raumbelastung der Reaktoren einen Einfluss auf die methanogenen Archaea zu haben. Jedoch ließ sich ein negativer Einfluss hoher Ammonium- bzw. Am-moniakkonzentrationen auf das Wachstum acetoklastischer Methanbildner, insbesondere Methanosaeta spp., in den untersuchten landwirtschaftlichen Biogasanlagen feststellen. Die aus dieser Studie gewonnenen Erkenntnisse dienen dem grundlegenden Verständnis der methanoge-nen Lebensgemeinschaft in landwirtschaftlichen Biogasanlagen, und sollen somit zur weiteren Optimierung der Biogasgewinnung beitragen. Biogas production from agricultural main products or remnants provides a substantial contribution to the reduction of CO2 emissions but also to the development of a sustainable agriculture. Currently, the re-search focuses on development and optimization of biogas reactors and reactor performance as well as optimization of substrate eligibility. However, the knowledge of the structure and dynamics of microbial communities which are involved in the biogas production is still insufficient. Within this study, the structures of methanogenic biocoenosis in ten agricultural biogas plants which are operated on basis of renewable raw materials were analyzed. Therefore a polyphasic approach with differ-ent culture independent molecular genetic techniques was chosen. First, PCR-RFLP analyses of 16S rDNA and mcrA genes were conducted. For determination of the relative abundance of methane pro-ducing microorganisms in the samples a quantitative real-time PCR (Q-PCR) on basis of 16S rDNA group-specific primers was applied. Fluorescence in situ hybridization (FISH) was carried out for direct quantification of methanogenic cells in reactor contents of six of the ten biogas plants. FISH was carried out with specific probes for the domains Bacteria (EUB338) and Archaea (ARCH915), the orders Metha-nomicrobiales (MG1200) and Methanobacteriales (MB311 and MB1174) and the families Methanosarcinaceae (Ms821) and Methanosaetaceae (Mx825). The results of the analyses showed, that in nine of the ten biogas plants the hydrogenotrophic methano-gens, represented by the orders Methanomicrobiales and Methanobacteriales, were prevalent. The genus Methanoculleus (Methanomicrobiales) was the predominating genus in these biogas plants. Acetoclastic methanogens such as organisms of the genus Methanosaeta were found only in six of the ten analyzed biogas plants. However, in one biogas plant Methanosaeta was determined as the predominant methano-genic group. These results indicate that the hydrogenotrophic methanogenesis was the major metabolic pathway for methane production in 90 % of the analyzed biogas plants. In this study, no correlation between used substrates for biogas production and the structure of Archaea biocoenosis could be observed. Also, the retention time of substrates and the organic loading rate of reac-tors seemed to have no obvious effect on the methanogenic Archaea. However, in the analyzed biogas plants a negative influence of high ammonium and ammonia concentrations, respectively, on the growth of acetoclastic methanogens in particular Methanosaeta spp. was determined. The results of this study serve the basic understanding of the methanogen community in agricultural bio-gas plants and are to contribute to further optimization of biogas production.

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    https://dx.doi.org/10.14279/de...
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