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This work investigates the influence of the positive regulator XYR1 of Trichoderma harzianum on the production of cellulolytic enzymes, using sugarcane bagasse as carbon source. Constitutive expression of xyr1 was achieved under the control of the strong Trichoderma reesei pki1 promoter. Five clones with xyr1 overexpression achieved higher xyr1 expression and greater enzymatic productivity when cultivated under submerged fermentation, hence validating the genetic construction for T. harzianum. Clone 5 presented a relative expression of xyr1 26-fold higher than the parent strain and exhibited 66, 37, and 36% higher values for filter paper activity, xylanase activity, and β-glucosidase activity, respectively, during cultivation in a stirred-tank bioreactor. The overexpression of xyr1 in T. harzianum resulted in an enzymatic complex with significantly improved performance in sugarcane bagasse saccharification, with an enhancement of 25% in the first 24h. Our results also show that constitutive overexpression of xyr1 leads to the induction of several important players in biomass degradation at early (24h) and also late (48h) timepoints of inoculation. However, we also observed that the carbon catabolite repressor CRE1 was upregulated in xyr1 overexpression mutants. These findings demonstrate the feasibility of improving cellulase production by modifying regulator expression and suggest an attractive approach for increasing total cellulase productivity in T. harzianum.

Neutron-induced ssion cross-sections of minor actinides have been measured using the n TOF white neutron source at CERN, Geneva, as part of a large experimental program aiming at collecting new data relevant for nuclear astrophysics and for the design of advanced reactor systems. The measurements at n TOF take advantage of the innovative features of the n TOF facility, namely the wide energy range, high instantaneous neutron ux and good energy resolution. Final results on the ssion cross-section of 233U, 245Cm and 243Am from thermal to 20 MeV are here reported, together with preliminary results for 241Am. The measurement have been performed with a dedicated Fast Ionization Chamber (FIC), a ssion fragment detector with a very high e ciency, relative to the very well known cross-section of 235U, measured simultaneously with the same detector.

Biomass subpopulations in mammalian cell culture processes cause impurities and influence productivity, which requires this critical process parameter to be monitored in real-time.For this reason, a novel soft sensor concept for estimating viable, dead and lysed cell concentration was developed, based on the robust and cheap in situ measurements of permittivity and turbidity in combination with a simple model. It could be shown that the turbidity measurements contain information about all investigated biomass subpopulations. The novelty of the developed soft sensor is the real-time estimation of lysed cell concentration, which is directly correlated to process-related impurities such as DNA and host cell protein in the supernatant. Based on data generated by two fed-batch processes the developed soft sensor is described and discussed.The presented soft sensor concept provides a tool for viable, dead and lysed cell concentration estimation in real-time with adequate accuracy and enables further applications with respect to process optimization and control.

The paper investigates the European space heating (SH) and domestic hot water (DHW) market in order to close knowledge gaps concerning its size. The stimulus for this research arises from incongruences found in SH and DHW market’s data in spite of over two decades of scientific research. The given investigation has been carried out in the framework of the Hotmaps project (Horizon 2020—H2020), which aims at designing an open source toolbox to support urban planners, energy agencies, and public authorities in heating and cooling (H&C) planning on country, regional, and local levels. Our research collects and analyzes SH and DHW market data in the European Union (EU), specifically the amount of operative units, installed capacities, energy efficiency coefficients as well as equivalent full-load hours per equipment type and country, with a bottom-up approach. The analysis indicates that SH and DHW account for a significant portion of the total EU energy utilization (more than 20%), amounting to almost 3900 TWh/y. At the same time, the energy consumption provided by district heating (DH) systems exceeds the one of condensing boilers. While DH systems applications are growing throughout the EU, the replacement of elderly, conventional boilers progresses at a slower pace.

Strain degeneration in solventogenic clostridia is a known problem in the technical acetone-butanol fermentation bioprocess, especially in the continuous process mode. Clostridial strain degeneration was studied by Fourier transform infrared (FT-IR) spectroscopy of the bacterial cells. Degenerative variant formation in two strains, Clostridium beijerinckii NCIMB 8052 and Clostridium species AA332, was detected spectroscopically. Colonies on solid media were sampled, or assayed directly in situ by IR microscopy. It has previously been shown that the distinctive acidogenic and solventogenic physiological phases of Clostridium acetobutylicum in liquid medium can be discriminated by FT-IR spectroscopy. This was confirmed here for C. beijerinckii NCIMB 8052. The proportion of degenerate cells in a mixed population in liquid medium could be quantified, as the spectral features change in different ways during the normal growth cycle of wild type organisms and degenerate variants in batch culture. This opens a new perspective for physiology-based process monitoring and control, especially of the continuous acetone-butanol fermentation.

Abstract A general numerical model is presented here to describe the complex fluid dynamics and the heat transfer process in high-temperature circulating fluidized beds (CFBs). The core-wall concept is used to describe the gas-solid flow in the dilute phase section of CFBs. The variation of the thickness of the wall layer along the height direction is considered in the fluid dynamic model in order to approach the practical conditions. Three components of heat transfer, i.e., the particle-convective heat transfer, the gas-convective heat transfer, and the radiative heat transfer, and their contributions to the total heat transfer coefficient are investigated. The influences of some operating parameters on the total heat transfer and its components are predicted. Detailed information about the mechanism of heat transfer is discussed. The radiative heat transfer accounts for about 30∼60% of the total heat transfer in high temperature CFBs. It gradually increases along the height direction of the furnace. When the contribution of particle convection increases, the contribution of gas convection decreases, and vice versa. Particle size shows a significant effect on the radiative heat transfer and the convective heat transfer. High bed and wall temperatures will primarily increase the radiative heat transfer.

This study fills in knowledge gaps for the European air-conditioning (AC) market, which is fundamentally important to raising awareness about primary energy utilization. In contrast to space heating (SH) and domestic hot water (DHW) preparation, the European Union (EU) AC market is barely explored in scientific literature. While the focus of previous research has been on the residential sector, a shortfall of data for the services (wholesale and retail, offices, education, health, hotels and bars) exists. In this paper, data describing the actual space cooling (SC) market in Europe (quantity of SC units, equivalent full-load hours, installed capacities, seasonal energy efficiency values as well as cooled floor area per AC type and/or sector) is collected and explored using a bottom-up approach. Results indicate that SC is responsible for a significant portion of EU electricity consumption in households (nearly 5%) and even more in the service sector (~13%). Energy consumption for SC in the EU28 appears to be more than 140 TWh/y. The quantification of the European AC consumption shows a significant difference between the service and residential sectors: about 115 versus 25 TWh/y respectively. The SC market in Europe is characterized by a high potential for growth, especially in households.