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The severity of drought is predicted to increase across Europe due to climate change. Droughts can substantially impact terrestrial nitrogen (N) cycling and the corresponding microbial communities. Here, we investigated how ammonia-oxidizing bacteria (AOB), archaea (AOA), and complete ammonia oxidizers (comammox) as well as inorganic N pools and N2O fluxes respond to simulated drought under different cropping systems. A rain-out shelter experiment was conducted as part of a long-term field experiment comparing cropping systems that differed mainly in fertilization strategy (organic, mineral, or mixed mineral and organic) and plant protection management (biodynamic versus conventional pesticide use). We found that the effect of drought varied depending on the specific ammonia-oxidizing (AO) groups and the type of cropping system. Drought had the greatest impact on the structure of the AOA community compared to the other AO groups. The abundance of ammonia oxidizers was also affected by drought, with comammox clade B exhibiting the highest sensitivity. Additionally, drought had, overall, a stronger impact on the AO community structure in the biodynamic cropping system than in the mixed and mineral-fertilized conventional systems. The responses of ammonia-oxidizing communities to drought were comparable between bulk soil and rhizosphere. We observed a significant increase in NH4+ and NO3− pools during the drought period, which then decreased after rewetting, indicating a strong resilience. We further found that drought altered the complex relationships between AO communities and mineral N pools, as well as N2O fluxes. These results highlight the importance of agricultural management practices in influencing the response of nitrogen cycling guilds and their processes to drought. Soil Biology and Biochemistry, 201 ISSN:0038-0717 ISSN:1879-3428

Data management plan for the grant, "Non-Genetic Inheritance of Hypoxia Tolerance in Fishes: Dynamics and Mechanisms." Research quantifying the inheritance of tolerance to low oxygen in a model fish and then determine the tolerance mechanisms, at organismal to molecular levels, that are passed on from parents to their offspring. The investigators will not only focus on conventional, well-studied genetic mechanisms for inheritance, but will explore so-called “epigenetic” forms of inheritance that may transfer parental characteristics for only a generation or two. Such “temporary inheritance” might actually require less energy and be more beneficial to a species than the more permanent form of genetic inheritance. This project will quantify non-genetic inheritance of hypoxia tolerance in zebrafish as a model organism and then identify underlying mechanisms, at organismal to molecular levels, in parents and in their progeny. Specifically, this project will quantify non-genetically inherited traits that allow hypoxia tolerance, determine “wash-in” and “wash-out” (i.e., the dynamics) of hypoxia-tolerant phenotypes across multiple generations, and establish epigenetic mechanism(s) of non-genetic inheritance in subsequent generations. The information provided by this project will allow biologists to better predict, and perhaps even mitigate, the negative consequences of future episodes of low oxygen in rivers and lakes.

This paper studies the long-term energy management of a microgrid coordinating hybrid hydrogen-battery energy storage. We develop an approximate semi-empirical hydrogen storage model to accurately capture the power-dependent efficiency of hydrogen storage. We introduce a prediction-free two-stage coordinated optimization framework, which generates the annual state-of-charge (SoC) reference for hydrogen storage offline. During online operation, it updates the SoC reference online using kernel regression and makes operation decisions based on the proposed adaptive virtual-queue-based online convex optimization (OCO) algorithm. We innovatively incorporate penalty terms for long-term pattern tracking and expert-tracking for step size updates. We provide theoretical proof to show that the proposed OCO algorithm achieves a sublinear bound of dynamic regret without using prediction information. Numerical studies based on the Elia and North China datasets show that the proposed framework significantly outperforms the existing online optimization approaches by reducing the operational costs and loss of load by around 30% and 80%, respectively. These benefits can be further enhanced with optimized settings for the penalty coefficient and step size of OCO, as well as more historical references. Submitted to Applied Energy

In the quest for effective solutions to address Environ. Pollut. and meet the escalating energy demands, heterojunction photocatalysts have emerged as a captivating and versatile technology. These photocatalysts have garnered significant interest due to their wide-ranging applications, including wastewater treatment, air purification, CO2 capture, and hydrogen generation via water splitting. This technique harnesses the power of semiconductors, which are activated under light illumination, providing the necessary energy for catalytic reactions. With visible light constituting a substantial portion (46%) of the solar spectrum, the development of visible-light-driven semiconductors has become imperative. Heterojunction photocatalysts offer a promising strategy to overcome the limitations associated with activating semiconductors under visible light. In this comprehensive review, we present the recent advancements in the field of photocatalytic degradation of contaminants across diverse media, as well as the remarkable progress made in renewable energy production. Moreover, we delve into the crucial role played by various operating parameters in influencing the photocatalytic performance of heterojunction systems. Finally, we address emerging challenges and propose novel perspectives to provide valuable insights for future advancements in this dynamic research domain. By unraveling the potential of heterojunction photocatalysts, this review contributes to the broader understanding of their applications and paves the way for exciting avenues of exploration and innovation.

The importance of climate change for driving adverse climate impacts has motivated substantial effort to understand the rate and magnitude of regional climate change in different parts of the world. However, despite decades of research, there is substantial uncertainty in the time remaining until specific regional temperature thresholds are reached, with climate models often disagreeing both on the warming that has occurred to-date, as well as the warming that might be experienced in the next few decades. Here, we adapt a recent machine learning approach to train a convolutional neural network to predict the time (and its uncertainty) until different regional warming thresholds are reached based on the current state of the climate system. In addition to predicting regional rather than global warming thresholds, we include a transfer learning step in which the climate-model-trained network is fine-tuned with limited observations, which further improves predictions of the real world. Using observed 2023 temperature anomalies to define the current climate state, our method yields a central estimate of 2040 or earlier for reaching the 1.5 degrees C threshold for all regions where transfer learning is possible, and a central estimate of 2040 or earlier for reaching the 2.0 degrees C threshold for 31 out of 34 regions. For 3.0 degrees C, 26 degrees C out of 34 regions are predicted to reach the threshold by 2060. Our results highlight the power of transfer learning as a tool to combine a suite of climate model projections with observations to produce constrained predictions of future temperatures based on the current climate. Environmental Research Letters, 20 (1) ISSN:1748-9326 ISSN:1748-9318

Lake ecosystems are extremely sensitive to nitrogen growth, which leads to water quality degradation and ecosystem health decline. Nitrogen depositions, as one of the main sources of nitrogen in water, are expected to change under future climate change scenarios. However, it remains not clear how nitrogen deposition to lakes respond to future meteorological conditions. In this study, a source-oriented version of Community Multiscale Air Quality (CMAQ) Model was used to estimate nitrogen deposition to 263 lakes in 2013 and under three RCP scenarios (4.5, 6.0 and 8.5) in 2046. Annual total deposition of 58.2 Gg nitrogen was predicted for all lakes, with 23.3 Gg N by wet deposition and 34.9 Gg N by dry deposition. Nitrate and ammonium in aerosol phase are the major forms of wet deposition, while NH3 and HNO3 in gas phase are the major forms of dry deposition. Agriculture emissions contribute to 57% of wet deposition and 44% of dry deposition. Under future meteorological conditions, wet deposition is predicted to increase by 5.5% to 16.4%, while dry deposition would decrease by 0.3% to 13.0%. Changes in wind speed, temperature, relative humidity (RH), and precipitation rates are correlated with dry and wet deposition changes. The predicted changes in deposition to lakes driven by meteorological changes can lead to significant changes in aquatic chemistry and ecosystem functions. Apart from future emission scenarios, different climate scenarios should be considered in future ecosystem health evaluation in response to nitrogen deposition.

This paper investigates the use of deep multi-agent reinforcement learning (MARL) for the coordination of residential energy flexibility. Particularly, we focus on achieving cooperation between homes in a way that is fully privacy-preserving, scalable, and that allows for the management of distribution network voltage constraints. Previous work demonstrated that MARL-based distributed control can be achieved with no sharing of personal data required during execution. However, previous cooperative MARL-based approaches impose an ever greater training computational burden as the size of the system increases, limiting scalability. Moreover, they do not manage their impact on distribution network constraints. We therefore adopt a deep multi-agent actor-critic method that uses a centralised but factored critic to rehearse coordination ahead of execution, such that homes can successfully cooperate at scale, with only first-order growth in computational time as the system size increases. Training times are thus 34 times shorter than with a previous state-of-the-art reinforcement learning approach without the factored critic for 30 homes. Moreover, experiments show that the cooperation of agents allows for a decrease of 47.2% in the likelihood of under-voltages. The results indicate that there is significant potential value for management of energy user bills, battery depreciation, and distribution network voltage management, with minimal information and communication infrastructure requirements, no interference with daily activities, and no sharing of personal data. Applied Energy, 377, Part A ISSN:0306-2619 ISSN:1872-9118

[This dataset contains all data used for Studies 2 (qualitative), 3 (quantitative survey) and 4 (longitudinal) in my PhD research.]<br>Thesis abstract:This thesis explores the potential positive impact of artificial intelligence (AI) technology on sustainability in and outside of the tourism industry through four studies. Study 1 introduced the AI4GoodTourism framework, emphasising the need for sustainability inclusion</em> and tourist involvement</em> to achieve a successful sustainability transition. Five themes were identified through a systematic review: intelligent automation to enhance tourist experience, preserve heritage, promote quality of life, measure tourist experience, and preserve the environment. The latter theme was the least explored scholarly topic. Study 2 conceptualised a conversational AI chatbot to promote pro-environmental behaviour spillover among tourists visiting the Gili Islands, Indonesia. A theoretical model was proposed, highlighting factors influencing chatbot usage and spillover effects. Study 3 identified relationships between factors from Study 2, revealing that factors such as performance expectancy, timing, </em>and credibility</em> significantly influenced people’s intention to use the proposed chatbot technology. A significant relationship was established between people’s intentions to use the chatbot and environmentally friendly transport. Scenario-based experiments showed that using the chatbot with educational information on sustainability was sufficient to trigger behaviour change. Study 4 explored the underlying mechanism of pro-environmental behaviour spillover through human-chatbot interactions using flashback nudging. A longitudinal experiment involving the Gili tourists demonstrated that flashback nudging delivered through chatbot technology strengthened their environmental self-identity, leading to significant differences in self-reported pro-environmental behaviour between treatment and control groups. In conclusion, the thesis demonstrates that AI technology, designed with high sustainability inclusion, can positively impact sustainability through tourists’ marginal contributions. The proposed AI4GoodTourism framework and the conceptualised chatbot technology, especially with flashback nudging, show potential for facilitating pro-environmental behaviour spillovers among tourists. All four studies in this thesis highlight the importance of prioritising sustainability in AI innovations for the tourism industry, offering insights for future AI development and adoption to support the global sustainability agenda.

# Data from: Evaluating the effects of cotton intercropping on cool-season perennial forage persistence, forage mass, and nutritive value in the southeastern United States This dataset was used to generate 3 figures and 5 tables in the publication, "Evaluating the effects of cotton intercropping on cool-season perennial forage persistence, forage mass, and nutritive value in the southeastern United States". All data was collected in Florence, South Carolina at the Clemson University Pee Dee Research and Education Center during 2021 and 2022. \#Description of dataset and file structure Data is presented in an Microsoft Excel Spreadsheet, with separate tabs for datasets related to each of the 3 figures/subfigures and 5 tables in the published manuscript. For all data the following treatment abbreviations are used: Fallow = weedy, unplanted treatment ARG = annual ryegrass RC+WC = 50/50 mixture of red and white clover ARG+RC+WC = 50% annual ryegrass, 25% red clover, and 25% white clover **Data for Figure 1** These data were used to generate Figure 1, featuring mean weather data for the study years, 2021 & 2022, along with 30-year mean weather data for the nearest NOAA weather station (Florence, SC Regional Airport). Units are provided in the column headers. **Data for Figure 2a & 2b** These data were used to generate Figures 2a and 2b, featuring the amount of spring forage mass accumulation preceding and in between cotton intercropping. Forage mass in the RC+WC and ARG+RC+WC treatments consited of a mix of weeds and clovers, while ARG and fallow treatments are entirely comprised of weedy biomass **Data for Figure 3a & 3b** These data were used to generate Figures 3a and 3b, featuring the red and white clover populations in each treatment for each year of the study. **Data for Figure 3c** These data were used to generate figure 3c, featuring the weedy species population changes from spring to fall before, between, and after two seasons of cotton intercropping in 2021 and 2022.Final data in the published figure was Weeds per square meter. **Data for Tables 1 and 2** These data were used to generate Tables 1 & 2, featuring height data for individual clover, annual ryegrass, and weedy species observed among different treatments. **Data for Table 3** These data were used to generate part of table 3, featuring the forage nutritive value data (crude protien, CP; acid detergent fiber, ADF; neutral detergent fiber, NDF; non-fibrous carbohydrates, NFC; total digestible nutrients, TDN; net energy of lactation, NEL; net energy of maintenance, NEM; net energy of gain, NEG) **Data for Tables 3, 4, and 5** These data were used to generate part of Table 3, and Tables 4 and 5, featuring nutrient compositions of the forage plant tissues collected during the trial. All data are in g/kg dry matter. ## Sharing Access Information These data were originated from the published manuscript: [https://doi.org/10.1002/agj2.21625](https://doi.org/10.1002/agj2.21625). This is digital research data corresponding to a published manuscript, Evaluating the effects of cotton intercropping on cool-season perennial forage persistence, forage mass, and nutritive value in the southeastern United States, in Agronomy Journal. Integrated forage–row cropping systems provide important agronomic and economic benefits to producers. However, little attention has been given to incorporating forages into row crop systems unique to the southeastern United States. This study assessed the viability of intercropping cotton (Gossypium hirsutum L.) on perennial, cool-season legumes during the summer months in the Southeast Coastal Plain over two production years. Treatments included a weedy fallow, annual ryegrass (ARG; Lolium multiflorum Lam.) monoculture, a red clover (RC; Trifolium pratense L.) and white clover (WC; Trifolium repens L.) mixture, and a three-species mixture of ARG, RC, and WC. Plots were established in fall 2020 with forage grown until May 2021 and 2022, when plots were strip-tilled and planted with cotton. Cotton was managed with minimal herbicide use to preserve perennial clovers. Data was collected over two years (October 2020 - October 2022) at the Clemson Pee Dee Research and Education Center near Florence, SC. Data was collected by field measurements of plant height, biomass accumulation, and species persistence and diversity, with laboratory assays conducted to collect plant nutritional composition. Forage nutrtitive value parameters and fiber content were conducted by a third-party laboratory (Dairy One LLC, Ithaca, NY).