• Energy Research

The supply of waste glycerol is rising steadily, partially due to the increased global production of biodiesel. Global biodiesel production totals about 47.1 billion liters and is a process that involves the co-production of waste glycerol, which accounts for over 12% of total esters produced. Waste glycerol is also generated during bioethanol production and is estimated to account for 10% of the total sugar consumed on average. Therefore, there is a real need to seek new technologies for reusing and neutralizing glycerol waste, as well as refining the existing ones. Biotechnological means of valorizing waste glycerol include converting it into gas biofuels via anaerobic fermentation processes. Glycerol-to-bioenergy conversion can be improved through the implementation of new technologies, the use of carefully selected or genetically modified microbial strains, the improvement of their metabolic efficiency, and the synthesis of new enzymes. The present study aimed to describe the mechanisms of microbial and anaerobic glycerol-to-biogas valorization processes (including methane, hydrogen, and biohythane) and assess their efficiency, as well as examine the progress of research and implementation work on the subject and present future avenues of research.

Recent years have brought significant evolution and changes in wastewater treatment systems. New solutions are sought to improve treatment efficiency, reduce investment/operational costs, and comply with the principles of circular economy and zero waste. Microbial granules can serve as an alternative to conventional technologies. Indeed, there has been fast-growing interest in methods harnessing aerobic (AGS) and anaerobic (AnGS) granular sludge as well as microbial-bacterial granules (MBGS), as evidenced by the number of studies on the subject and commercial installations developed. The present paper identifies the strengths and weaknesses of wastewater treatment systems based on granular sludge (GS) and their potential for energy production, with a particular focus on establishing the R&D activities required for further advance of these technologies. In particular, the impact of granules on bioenergy conversion, including bio-oil recovery efficiency and biomethane/biohydrogen yields, and bioelectrochemical systems must be assessed and optimized.

The technology of aerobic granular sludge (AGS) seems prospective in wastewater bio-treatment. The characteristics as well as compactness and structure of AGS have been proved to significantly affect the effectiveness of thus far deployed methods for sewage sludge processing, including anaerobic digestion (AD). Therefore, it is deemed necessary to extend knowledge on the possibilities of efficient AGS management and to seek viable technological solutions for methane fermentation of sludge of this type, including by means of using the pre-treatment step. Little is known about the pre-treatment method with solidified carbon dioxide (SCO2), which can be recovered in processes of biogas upgrading and enrichment, leading to biomethane production. This study aimed to determine the impact of AGS pre-treatment with SCO2 on the efficiency of its AD. An energy balance and a simplified economic analysis of the process were also carried out. It was found that an increasing dose of SCO2 applied in the pre-treatment increased the concentrations of COD, N-NH4+, and P-PO43− in the supernatant in the range of the SCO2/AGS volume ratios from 0.0 to 0.3. No statistically significant differences were noted above the latter value. The highest unit yields of biogas and methane production, reaching 476 ± 20 cm3/gVS and 341 ± 13 cm3/gVS, respectively, were obtained in the variant with the SCO2/AGS ratio of 0.3. This experimental variant also produced the highest positive net energy gain, reaching 1047.85 ± 20 kWh/ton total solids (TS). The use of the higher than 0.3 SCO2 doses was proved to significantly reduce the pH of AGS (below 6.5), thereby directly diminishing the percentage of methanogenic bacteria in the anaerobic bacterial community, which in turn contributed to a reduced CH4 fraction in the biogas.

One of the most effective technologies involving the use of lignocellulosic biomass is the production of biofuels, including methane-rich biogas. In order to increase the amount of gas produced, it is necessary to optimize the fermentation process, for example, by substrate pretreatment. The present study aimed to analyze the coupled effects of microwave radiation and the following acids: phosphoric(V) acid (H3PO4), hydrochloric acid (HCl), and sulfuric(VI) acid (H2SO4), on the destruction of a lignocellulosic complex of maize silage biomass and its susceptibility to anaerobic degradation in the methane fermentation process. The study compared the effects of plant biomass (maize silage) disintegration using microwave and conventional heating; the criterion differentiating experimental variants was the dose of acid used, i.e., 10% H3PO4, 10% HCl, and 10% H2SO4 in doses of 0.02, 0.05, 0.10, 0.20, and 0.40 g/gTS. Microwave heating caused a higher biogas production in the case of all acids tested (HCl, H2SO4, H3PO4). The highest biogas volume, exceeding 1800 L/kgVS, was produced in the variant with HCl used at a dose of 0.4 g/gTS.

One of the identified obstacles to the development of biogas plants is the limited sources of organic substrates. Hydrophytes varying in morphology, chemical composition, and origin proved to be viable alternatives. This study’s aim was to determine the feasibility of deploying biomass from the cleansing of water reservoirs for anaerobic digestion. A mass and energy balance was estimated for Warmia–Mazury Province (Poland), the so-called Thousand Lake District. The effectiveness of anaerobic digestion was determined in anaerobic batch respirometric reactors. The biomass of hydrophytes harvested from this area approximated 38,070 Mg FM/year. The biogas yield from emergent plants approximated 350 m3/MgVS, and the average CH4 content of biogas reached 55%, whereas the respective values obtained from submerged biomass reached 270 m3/MgVS and 58% CH4. The total CH4 volume achievable from the facility fed with waste biomass of this type allows producing ca. 1,654,560 m3CH4/year, which enables reaching 866 kW theoretical thermal power and 636 kWe net electrical power with the facility. The contribution of biomass from mowing in this energy effect was about 84%, whereas that of the submerged vegetation obtained by hacking the bottom was 16%.

This study aimed at evaluating the methane potential of two ultrafiltration (UFP) and two diafiltration (DFP) permeates generated during milk protein concentration. The permeates were characterized by a different chemical oxygen demand (COD) ranging from 7610 mg O2/L to 57,020 mg O2/L. The CH4 production efficiency was recorded for 20 days and ranged from 149 to 181 NL/kg CODadded. Moreover, the possibilities of the use of UFP/DFP to produce electricity and heat with a combined heat and power (CHP) unit was analyzed to underline the impact of the implementation of anaerobic digestion on the electric and thermal energy requirements of a dairy plant. It was concluded that the application of anaerobic digestion to UFP and DFP treatments generates the energy required to cover all the large-scale dairy plant energy demands and produce extra income. The amount of permeates generated annually in the analyzed dairy plant will enable the production of approx. 22,699 MWh of electricity and 85,516 GJ of heat. This would require a biogas plant with a 3 MW yield. Additionally, the lactose production from UFP/DFP was considered as an alternative or parallel solution for its management. The study confirmed that the biogas and lactose production from UFP/DFP enables plant owners to adjust a plant’s management towards one of these two solutions.

Photosynthetic microbial fuel cells (pMFCs) are hybrid systems that enable simultaneous wastewater treatment under anaerobic conditions and the generation of electricity by utilizing the potential difference in the anaerobic anode chamber and the oxygenated cathode chamber. Dairy wastewater with a concentration of 2000 mg COD/L was treated in the anode of a batch pMFC. In the cathode chamber, Chlorella vulgaris or Arthrospira platensis was cultivated in synthetic medium, and next in diluted effluent from the anode chamber. The highest power density of 91 mW/m2 was generated by the pMFC with the cultivation of Arthrospira platensis. Higher values of dissolved oxygen remained during the dark phase in the cathodic medium with Arthrospira platensis cultivation than with Chlorella vulgaris. This depletion of oxygen significantly decreased voltage generation, which during the light phase increased again to the maximum values. The COD removal achieved in the anodic chamber was 87%. The efficiency of nitrogen removal in the cathode chamber during the cultivation of Arthrospira platensis and Chlorella vulgaris was about 78% and 69%, respectively. The efficiency of phosphorus removal in the cathode chamber with the cultivation of Arthrospira plantensis and Chlorella vulgaris was 58% and 43%, respectively. This study has shown that the introduction of Arthrospira platensis into the cathode chamber is more effective than that of Chlorella vulgaris.

The most common technology for the recovery of energy and valuable materials from sewage sludge is anaerobic digestion (AD). Ensuring thermophilic conditions during AD has been proven to cause process intensification and an improvement in its final outcomes. Nonetheless, the search is underway for other methods to bolster the effectiveness of the AD of aerobic granular sludge (AGS), which is characterized by a compact and complex structure. A prospective AGS pre-treatment technology entails the use of solidified carbon dioxide (SCO2). The present study focused on an evaluation of the AGS pre-treatment with SCO2 on the thermophilic AD technological effects. It evaluated the effect of the SCO2 pre-treatment method on changes in the concentrations of organic and biogenic compounds in the dissolved phase and the yield and kinetics of biogas and methane production in periodical reactors, as well as enabled the development of an empirical organizational model of biogas production. SCO2 introduced to AGS caused an increase in the content of COD, N-NH4+, and P-PO43− in the AGS dissolved phase at SCO2/AGS volumetric ratios ranging from 0 to 0.3. A further increase in the SCO2 dose did not cause any statistically significant differences in this respect. The highest biogas and methane yields were obtained at SCO2/AGS of 0.3 and reached 482 ± 21 cm3/gVS and 337 ± 14 cm3/gVS, respectively. The higher SCO2 doses used led to a significant decrease in the pH value of the AGS, which, in turn, contributed to a decreasing CH4 concentration in the biogas.

The conversion of chemical energy contained in organic matter into electricity has become an object of interest for many scientists worldwide. This technology is used in microbial fuel cells (MFC). Apart from generating electrical energy, these cells can be used simultaneously for wastewater treatment. Although the technology is constantly being improved, currently functioning microbial fuel cells cannot provide appropriate output parameters to use on an industrial scale. One of the barriers is so-called extracellular electron transfer, which in turn depends on the electrode type used, its material, shape, and size. According to current literature, carbon, graphite, stainless steel, and ceramics are the most frequently used electrode materials. However, more and more often, scientists are turning to other, unusual materials, the production of which uses the newest technologies, and one of them is graphene. This material is modified in different ways and connected with other materials, and the results of this seem to be very promising. Scientists manage to get a higher level of extracellular electron transfer and, hence, higher output parameters of the whole system. This article describes chosen technologies and attempts made by scientists worldwide to use graphene in MFC and their results.