• Energy Research

This work demonstrates that cork used as oil-spill sorbents, contaminated with liquid hydrocarbons, herein demonstrated with hexadecane, can be biologically treated by Rhodococcus opacus B4 with concomitant lipids production. R. opacus B4 consumed up to 96% of hexadecane (C16) impregnated in natural and regranulated cork sorbents after 48h incubation, producing 0.59±0.06g of triacylglycerol (TAG) g-1 of C16 consumed with a TAG content of 0.60±0.06gg-1 of cellular dry weight (CDW) and 0.54±0.05g TAG g-1 of C16 consumed with a TAG content of 0.77±0.04gg-1 (CDW), respectively. TAG was mainly composed by fatty acids of 16 and 18 carbon chains demonstrating the feasibility of using it as raw material for biodiesel production. In addition, the obtained lipid-rich biomass (whole cells) can be used for biomethane production, at a yield of 0.4L CH4 g-1 (CDW). The obtained results support a novel approach for management of oil-spill contaminated cork sorbents through its valorisation by producing bacterial lipids, which can be used as feedstocks for biofuels production.

Ulex europaeus is one of the world worst invaders vegetal species and its suitability for biogas production is significant. The effect of three factors affecting the Biochemical Methane Potential (BMP, expressed as volume of CH4 per mass of volatile solids of waste) and the biodegradability rate (k, expressed in volume of CH4 per mass of VS and time) of U. europaeus was assessed by a Central Composite Face Centred Design. The BMP varied from 153 L kg−1 to 308 L kg−1. Inoculum to substrate ratio (ISR) and the type of inoculum had high influence on the final results. k varied from 14 L kg−1 d−1 to 49 L kg−1 d−1. The conditions that simultaneously maximized the BMP and k were an inoculum consisting in 55% (v) of granular sludge and 45 % (v) of suspended sludge from a sludge digester, an ISR of 4 g g−1, and a particle size of 1.9 mm. Considering the average biomass production in shrub land areas, the potential energy production from U. europaeus is estimated in (36.9 ± 19.3) GJ ha−1 yr−1. For example, in Europe, a maximum energy supply of 7 EJ yr−1 could be achieved from potentially harvestable shrub land areas.

Two culture media were tested for the production of bacterial nanocellulose (BNC) under static culture fermentation, one containing molasses (Mol-HS), the other molasses and corn steep liquor (Mol-CSL), as a source of carbon and nitrogen, respectively. These are low-cost nutrients widely available, which provide very good BNC productivities. However, the use of these substrates generates wastewaters with high organic loads. Anaerobic digestion is one of the most promising treatments for industrial wastewaters with high organic loads since, beyond removal of the organic matter, it generates energy, in form of biogas. The wastewaters from BNC fermentation were thus evaluated for their biochemical methane potential through anaerobic digestion. For this, two wastewaters streams were collected: (i) the culture medium obtained after fermentation (WaF) and (ii) the WaF combined with BNC washing wastewaters (WaW). These two effluents-WaF and WaW-were characterized regarding their chemical oxygen demand, total nitrogen, total and volatile solids, to assess their suitability for anaerobic digestion. The biochemical methane potential of WaF and WaW from Mol-CSL wastewaters was (387 ± 14 L kg-1 VS) and (354 ± 4 L kg-1 VS), corresponding to a methanization percentage of (86.9 ± 3.1) % and (79.5 ± 0.9) %, respectively. After treatment, the chemical oxygen demand of WaF and WaW was reduced by (89.2 ± 0.4) and (88.7 ± 1.5), respectively. An exploratory test using an Upflow Anaerobic Sludge Blanket reactor for WaW treatment was also performed. The reactor was operated with a organic loading rate of [(6.5 ± 0.1) g L-1 d-1] and hydraulic retention time of 3.33 days, allowing a chemical oxygen demand removal of 58% of WaW. Results here obtained demonstrate, for the first time, the high potential of AD for the valorisation of the BNC fermentation wastewaters.

A central composite design circumscribed method was used to define the experimental conditions that improve the methane production rate (kCH4, liters of methane per kilogram of VS of waste added and per day) and the cumulative methane production (cMP, liters of methane per kilogram of VS of waste added) of the co-digestion of sewage sludge (SS) with crude glycerol (cGly) and waste frying oil (WFO). Three factors were selected, i.e., SS concentration, global co-substrate concentration, and mass fraction of cGly (xcGly) in a mixture of cGly and WFO (in chemical oxygen demand, COD). SS digestion without co-substrate reached a cMP of (294 ± 6) L·kg−1 and a kCH4 of (64 ± 1) L·kg−1·d−1, at standard temperature and pressure conditions and expressed relatively to the initial volatile solids. After statistical analysis, SS and co-substrate concentrations of 4.6 g·L−1 and 8.8 g·L−1 (in COD), respectively, with xcGly of 0.8, were defined to simultaneously boost cMP (91 % more) and kCH4 (3-fold increase). Application of these conditions would yield 214 MWh more in electricity per 1000 m3 of SS digested.

This work evaluates the influence of the inoculum type, the pre-consumption of the residual substrate and the ratio of blanks’ headspace volume to working volume (Hv Wv−1, 0.6 to 10) on Biochemical Methane Potential (BMP) measurements when methane is monitored by gas chromatography. Different inocula were tested: digested sewage sludge—DSS, granular sludge—GS and fresh dairy manure—DM. Microcrystalline cellulose was used as the substrate. BMP surpassed the maximum theoretical value (BMPmax = 414 L kg−1) when methane produced in the blanks was not discounted, showing that degassing cannot stand alone as an alternative to the procedure of discounting the inoculum’s background production. Still, when the residual substrate concentration is high (e.g., in DM), degassing is mandatory because methane produced from its digestion will conceal the methane produced from the substrate in the BMP determination. For inocula with a low residual substrate (e.g., GS), short degassing periods are recommended in order to avoid detrimental effects on methanogenic activity. For moderate residual substrate concentrations (e.g., DSS), BMP values closer to BMPmax (90–97%) were achieved after degassing and discounting the blanks with lower Hv Wv−1. For higher Hv ∙ Wv−1, less accurate quantification occurred, likely due to error propagation. Proper inoculum pre-incubation time and discounting the methane production from blanks with low Hv Wv−1 (adjusted according to the estimated background methane) are essential for accurate BMP determinations.

A design of experiments was applied to evaluate different strategies to enhance the methane yield of macroalgae Gracilaria vermiculophylla. Biochemical Methane Potential (BMP) of G. vermiculophylla after physical pre-treatment (washing and maceration) reached 481±9 L CH4 kg(-1) VS, corresponding to a methane yield of 79±2%. No significant effects were achieved in the BMP after thermochemical pre-treatment, although the seaweeds solubilisation increased up to 44%. Co-digestion with glycerol or sewage sludge has proved to be effective for increasing the methane production. Addition of 2% glycerol (w:w) increased the BMP by 18%, achieving almost complete methanation of the substrate (96±3%). Co-digestion of seaweed and secondary sludge (15:85%, TS/TS) increased the BMP by 25% (605±4 L CH4 kg(-1) VS) compared to the seaweed individual digestion.

Beer production generates by-products with high energy potential, namely trub (Tr, dead yeast from the fermentation) and spent grain (SG, smashed barley grains). This work investigates the biochemical methane potential (BMP, volume of methane produced per volatile solids of substrate—L kg−1) of these by-products, performing batch anaerobic biodegradability assays. Single substrates were evaluated as well as a mixture of Tr:SG (1:9, weight), in order to simulate the relative proportion generated in breweries. Tr reached the highest BMP [(515 ± 4) L kg−1], still, considering the total amount of by-product available, the mixture of Tr:SG proved to be more rewarding in terms of volume of methane produced. The co-digestion of Tr:SG with crude glycerol (cGly), which was chosen as a co-substrate to promote a synergetic effect on their biodegradability, was assessed by adding different amounts of cGly, up to 33% (in weight). The assay with 10% of cGly achieved the highest methane production [(573 ± 9) L kg−1] and biodegradability [(94 ± 2) %], evidencing its potential for energy generation. The co-digestion of these by-products presented a potential electricity production of 206 kWh per cubic meter of beer produced, being capable of serving 80% of the brewer’s energy needs for heating.