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Siderophores are low molecular weight secondary metabolites produced by microorganisms under low iron stress as a specific iron chelator. In the present study, a rhizospheric bacterium was isolated from the rhizosphere of sesame plants from Salem district, Tamil Nadu, India and later identified as Bacillus subtilis LSBS2. It exhibited multiple plant-growth-promoting (PGP) traits such as hydrogen cyanide (HCN), ammonia, and indole acetic acid (IAA), and solubilized phosphate. The chrome azurol sulphonate (CAS) agar plate assay was used to screen the siderophore production of LSBS2 and quantitatively the isolate produced 296 mg/L of siderophores in succinic acid medium. Further characterization of the siderophore revealed that the isolate produced catecholate siderophore bacillibactin. A pot culture experiment was used to explore the effect of LSBS2 and its siderophore in promoting iron absorption and plant growth of Sesamum indicum L. Data from the present study revealed that the multifarious Bacillus sp. LSBS2 could be exploited as a potential bioinoculant for growth and yield improvement in S. indicum.

Due to climate change and current efforts to reduce emissions in the construction sector, this study evaluates and discusses the results of a comparative cradle-to-grave Life Cycle Assessment (LCA), with a main focus on Global Warming Potential for functionally equivalent carbon-reinforced concrete (CRC) and steel-reinforced concrete (SRC) façade panels for the first time. The novelty of this study is the focus on construction waste and, in particular, the worst-case application of non-recycled construction waste. The use of CRC requires a lower concrete thickness than SRC because the carbon fiber reinforcement does not corrode, in contrast to steel reinforcement. Façade panels of the same geometrical dimensions and structural performance were defined as functional units (FU). Assuming an End-of-Life (EoL) scenario of 50% landfill and 50% recycling, the Global Warming Potential (GWP, given in CO2 equivalent (CO2e)) of the CRC façade (411–496 kg CO2e) is shown to perform better than or equal to the SRC façade (492 kg CO2e). Changing the assumption of CRC to a worst-case scenario, going fully to landfill and not being recycled (single life cycle), turns the GWP results in favor of the SRC façade. Assuming a 50-year service life for the SRC façade panel and relativizing the emissions to the years, the more durable CRC façade performs much better. Finally, depending on the system boundary, the assumed EoL and lifetime, CRC can represent a lower-emission alternative to a functionally equivalent component made of SRC. The most important and “novel” result in this study, which also leads to future research opportunities, is that delicate adjustments (especially concerning EoL scenarios and expected service life) can lead to completely different recommendations for decision-makers. Only by combining the knowledge of LCA experts, structural engineers, and builders optimal decisions can be made regarding sustainable materials and building components.

Is it true that a nuclear technology approach to generate electric energy offers a clean, safe, reliable and affordable, i.e., sustainable option? In principle yes, however a technology impact on the environment strongly depends on the actual implementation bearing residual risks due to technical failures, human factors, or natural catastrophes. A full response is thus difficult and can be given first when the wicked multi-disciplinary issues get well formulated and “resolved”. These problems are lying at the interface between: the necessary R&D effort, the industrial deployment and the technology impact in view of the environmental sustainability including the management of produced hazardous waste. As such, this problem is clearly of multi-dimensional nature. This enormous complexity indicates that just a description of the problem might cause a dilemma. The paper proposes a novel holistic approach applying Multi-Criteria Decision Analysis to assess the potential of nuclear energy systems with respect to a sustainable performance. It shows how to establish a multi-level criteria structure tree and examines the trading-off techniques for scoring and ranking of options. The presented framework allows multi-criteria and multi-group treatment. The methodology can be applied to support any pre-decisional process launched in a country to find the best nuclear and/or non-nuclear option according to national preferences and priorities. The approach addresses major aspects of the environmental footprint of nuclear energy systems. As a case study, advanced nuclear fuel cycles are analyzed, which were previously investigated by the Nuclear Energy Agency (NEA/OECD) expert group WASTEMAN. Sustainability facets of waste management, resource utilization and economics are in focus.

Soil salinity and the use of saline groundwater are two major constraints in crop production, which covers a ~1.0 billion ha area of arid and semi-arid regions. The improved drainage function of soil can modify the salty growing environment for higher agricultural production. The present study evaluated the effectiveness of cut-soiler-constructed rice residue-filled preferential shallow subsurface drainage (PSSD) to improve the drainage function and its effect on the yield, quality and plant–water relations of mustard over 2019–2021. Cut-soiler-simulated drains were made in a semi-controlled lysimeter (2 × 2 × 3; L*W*H m) as the main plot treatment in a double replicated split–split experiment with two soil types (subplot) and three irrigation water salinities (4, 8 and 12 dS m−1) as the sub-sub-plot treatment. The drainage volume of variable salinity (EC), dependent on the total water input, was substantially higher in the rainy season (April to October), i.e., 16.6, 7.76 and 12.0% during 2018, 2019 and 2020, with 1.7, 0.32 and 0.77 kg salt removal per lysimeter, compared to the post-rainy season. The mustard seed, straw and biological yields were improved by 31.4, 14.41 and 18.08%, respectively, due to a positive effect on plant–water relations. The mustard seeds produced in the cut-soiler-treated plots recorded higher oil, crude fiber and protein contents and a lower erucic acid content. The increase in salt load, by higher-salinity irrigation water, was also efficiently managed by using cut-soiler PSSD. It was found that the saline irrigation water up to 12.0 dS m−1 can be used under such PSSD without any extra salt loading. The present study showed the potential of cut-soiler PSSD in root zone salinity management by improving drainage in salt-affected arid regions.

The promotion of electric vehicles (EVs) is an important measure for dealing with climate change and reducing carbon emissions, which are widely agreed goals worldwide. Being an important operating mode for electric vehicle charging stations in the future, the integrated photovoltaic and energy storage charging station (PES-CS) is receiving a fair amount of attention and discussion. However, how to optimally configure photovoltaic and energy storage capacity to achieve the best economy is essential and a huge challenge to overcome. In this paper, based on the historical data-driven search algorithm, the photovoltaic and energy storage capacity allocation method for PES-CS is proposed, which determines the capacity ratio of photovoltaic and energy storage by analyzing the actual operation data, which is performed while considering the target of maximizing economic benefits. In order to achieve the proposed capacity allocation, the method is as follows: First, the economic benefit model of the charging stations is established, taking the net present value and investment payback period as evaluation indicators; then, by analyzing the operation data of the existing charging station with the target of maximizing economic benefits, the initial configuration capacity is obtained; finally, the capacity configuration is verified through a comprehensive case analysis for the actual operation data. The results show that the capacity configuration obtained through the data analysis features an optimized economic efficiency and photovoltaic utilization. The proposed method can provide a theoretical and practical basis for newly planned or improved large-scale charging stations.

This study evaluated the efficacy of enzyme induced calcite precipitation (EICP) in restricting the mobility of heavy metals in soils. EICP is an environmentally friendly method that has wide ranging applications in the sustainable development of civil infrastructure. The study examined the desorption of three heavy metals from treated and untreated soils using ethylene diamine tetra-acetic acid (EDTA) and citric acid (C6H8O7) extractants under harsh conditions. Two natural soils spiked with cadmium (Cd), nickel (Ni), and lead (Pb) were studied in this research. The soils were treated with three types of enzyme solutions (ESs) to achieve EICP. A combination of urea of one molarity (M), 0.67 M calcium chloride, and urease enzyme (3 g/L) was mixed in deionized (DI) water to prepare enzyme solution 1 (ES1); non-fat milk powder (4 g/L) was added to ES1 to prepare enzyme solution 2 (ES2); and 0.37 M urea, 0.25 M calcium chloride, 0.85 g/L urease enzyme, and 4 g/L non-fat milk powder were mixed in DI water to prepare enzyme solution 3 (ES3). Ni, Cd, and Pb were added with load ratios of 50 and 100 mg/kg to both untreated and treated soils to study the effect of EICP on desorption rates of the heavy metals from soil. Desorption studies were performed after a curing period of 40 days. The curing period started after the soil samples were spiked with heavy metals. Soils treated with ESs were spiked with heavy metals after a curing period of 21 days and then further cured for 40 days. The amount of CaCO3 precipitated in the soil by the ESs was quantified using a gravimetric acid digestion test, which related the desorption of heavy metals to the amount of precipitated CaCO3. The order of desorption was as follows: Cd > Ni > Pb. It was observed that the average maximum removal efficiency of the untreated soil samples (irrespective of the load ratio and contaminants) was approximately 48% when extracted by EDTA and 46% when extracted by citric acid. The soil samples treated with ES2 exhibited average maximum removal efficiencies of 19% and 10% when extracted by EDTA and citric acid, respectively. It was observed that ES2 precipitated a maximum amount of calcium carbonate (CaCO3) when compared to ES1 and ES3 and retained the maximum amount of heavy metals in the soil by forming a CaCO3 shield on the heavy metals, thus decreasing their mobility. An approximate improvement of 30% in the retention of heavy metal ions was observed in soils treated with ESs when compared to untreated soil samples. Therefore, the study suggests that ESs can be an effective alternative in the remediation of soils contaminated with heavy metal ions.

The conversion of methane into liquid biofuels using methane-consuming bacteria, known as methanotrophs, contributes to sustainable development, as it mitigates the problem of climate change caused by greenhouse gases and aids in producing cleaner and renewable energy. In the present research, an efficient methanotroph, Methylosarcina sp. LC-4, was studied as a prospective organism for biodiesel production using methane. The methane uptake rate by the organism was enhanced 1.6 times and 2.35 times by supplementing LC-4 with micronutrients, such as copper and tungstate, respectively. This unique ability of the isolated organism enables the deployment of methanotrophs-based processes in various industrial applications. A Plackett–Burman statistical (PBD) design was used to quantify the role of the micronutrients and other media components present in the nitrate minimal salt media (NMS) in biomass and fatty acid methyl esters (FAME) yields. Nitrate, phosphate, and tungstate had a positive effect, whereas copper, magnesium, and salinity had a negative effect. The modified NMS media, formulated according to the results from the PBD analysis, increased the FAME yield (mg/L) by 85.7%, with the FAME content of 13 ± 1% (w/w) among the highest reported in methanotrophs. The obtained FAME consisted majorly (~90%) of C14–C18 saturated and monounsaturated fatty acids, making it suitable for use as biodiesel.