Ionizing radiation-induced mutation of human cells with different DNA repair capacities
Copyright policy )Ionizing radiation-induced mutation of human cells with different DNA repair capacities
We have observed significant differences in the response to ionizing radiation of two closely related human cells lines, and now compare the effects on these lines of both low and intermediate LET radiation. Compared to TK6, WTK1 has an enhanced X-ray survival, and is also more resistant to cell killing by alpha-particles. The hprt locus is more mutable in WTK1 than in TK6 by both X-rays and alpha-particles. WTK1 is also more mutable by alpha-particles than by X-rays at the hprt locus. X-ray-induced mutation at the heterozygous tk locus in WTK1 is about 25 fold higher than in TK6, while alpha-particle-induced mutation is nearly 50 fold higher at this locus. Also, the slowly growing tk- mutants, which comprise the majority of spontaneous and X-ray-induced tk- mutants of TK6, were not induced significantly by alpha-particles. Previously, we showed that TK6 has a reduced capacity for recombination compared with WTK1, and therefore, these results indicate that recombinational repair may contribute to both cell survival and mutation-induction following exposure to ionizing radiation. Such a mechanism may aid cell survival, but could also result in increased deleterious effects such as the unmasking of recessive mutations in cancer suppresser genes.
- University of North Texas United States
- Direction de la Recherche Fondamentale France
- University of North Texas United States
- Atomic Energy and Alternative Energies Commission France
- Los Alamos National Laboratory United States
Hypoxanthine Phosphoribosyltransferase, DNA Repair, Cell Survival, Cells, Gene Conversion, Thymidine Kinase, Cell Line, Dna Repair, Radiation Effects On Biochemicals, Thermal, Animal Cells, Genetics, Humans, Linear Energy Transfer, Lymphocytes, Alpha Particles 550400, And Other Environ. Pollutant Effects On Living Orgs. And Biol. Mat, 59 Basic Biological Sciences, Cell Death, X-Rays, 63 Radiation, Gene Recombination Proteins, DNA, Alpha Particles, Biological Radiation Effects, Mutation, And Tissue Culture, 560120, Mutations, Spleen, Ionizing Radiations
Hypoxanthine Phosphoribosyltransferase, DNA Repair, Cell Survival, Cells, Gene Conversion, Thymidine Kinase, Cell Line, Dna Repair, Radiation Effects On Biochemicals, Thermal, Animal Cells, Genetics, Humans, Linear Energy Transfer, Lymphocytes, Alpha Particles 550400, And Other Environ. Pollutant Effects On Living Orgs. And Biol. Mat, 59 Basic Biological Sciences, Cell Death, X-Rays, 63 Radiation, Gene Recombination Proteins, DNA, Alpha Particles, Biological Radiation Effects, Mutation, And Tissue Culture, 560120, Mutations, Spleen, Ionizing Radiations
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