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Utilization of interferometric light microscopy for the rapid analysis of virus abundance in a river

descriptionPublicationkeyboard_double_arrow_right Article , Journal 01 Jun 2017 France Publisher:Elsevier BVJournal:Research in Microbiology, volume 168, pages 413-418 (issn: 0923-2508,

Authors: Martine Boccara; Martine Boccara; Catherine Vénien-Bryan; Y. Fedala; Y. Fedala; Albert-Claude Boccara; Céline Roose-Amsaleg; +1 Authors

doi: 10.1016/j.resmic.2017.02.004

pmid: 28263904

Utilization of interferometric light microscopy for the rapid analysis of virus abundance in a river

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Abstract

There is a constant need for direct counting of biotic nanoparticles such as viruses to unravel river functioning. We used, for the first time in freshwater, a new method based on interferometry differentiating viruses from other particles such as membrane vesicles. In the French Marne River, viruses represented between 42 and 72% of the particles. A spring monitoring in 2014 revealed their increase (2.1 × 107 to 2.1 × 108 mL-1) linked to an increase in algal biomass and diversity of bacterial plankton. Predicted virus size distributions were in agreement with transmission electron microscopy analysis suggesting a dominance of large viruses (≥60 nm).

Country

France

Related Organizations

French Institute for Research in Computer Science and Automation
France
PSL Research University
France
Laboratoire Parole et Langage
France
University of Paris
France
École Normale Supérieure
France

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Keywords

Membrane vesicle, Fresh Water, Cyanobacteria, [ SDV.MP.VIR ] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, [ SDE.IE ] Environmental Sciences/Environmental Engineering, [SDV.EE.ECO]Life Sciences [q-bio]/Ecology, environment/Ecosystems, Microscopy, Electron, Transmission, Rivers, [SDV.EE.ECO] Life Sciences [q-bio]/Ecology, environment/Ecosystems, Microscopy, Interference, Biomass, River, [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, [SDE.IE]Environmental Sciences/Environmental Engineering, Plankton, [ SDV.EE.ECO ] Life Sciences [q-bio]/Ecology, environment/Ecosystems, Virus, Microscopy, Fluorescence, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Viruses, [SDE.IE] Environmental Sciences/Environmental Engineering, Seasons, Interferometric light microscopy

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	citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	22
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	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Average