Toothbrushes are contaminated by micro-organisms which may be sources of infection, re-infection or cross infection. The object of this research is to test the efficacy of a proprietary toothbrush spray disinfectant (Brushtox) against specific test bacteria and fungi and to consider its potential.Three bacteria and two fungi representing a broad microbial spectrum with relevance to oral bacteria were used in four laboratory tests including an in vitro toothbrush disinfection test.Brushtox proved to be almost 100% effective in three tests and showed significant inhibitory action in the standard agar diffusion test.Brushtox is an effective disinfectant agent for bacteria and fungi on toothbrushes. The solution may be valuable in the control of a wide variety of oral infections and secondary infections, especially in frail or debilitated individuals or those on immuno-suppressive therapy, and also in healthy individuals.