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Expression of DnaK and GroEL homologs in Leuconostoc esenteroides in response to heat shock, cold shock or chemical stress

descriptionPublicationkeyboard_double_arrow_right Article , Journal 01 Aug 1995Publisher:Oxford University Press (OUP)Journal:FEMS Microbiology Letters, volume 131, pages 57-62 (issn: 0378-1097, eissn: 1574-6968,

Authors: Deotosh Kumar Singh; Narayanan Krishna; Howard Jaffe; Kamakhya Prasad Seal; Poonam Salotra; Rakesh Bhatnagar;

doi: 10.1111/j.1574-6968.1995.tb07754.x

pmid: 7557310

Expression of DnaK and GroEL homologs in Leuconostoc esenteroides in response to heat shock, cold shock or chemical stress

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Abstract

The mechanism of adaptation of bacteria to survive at elevated temperature in the human host and the expression of heat-shock proteins in response to stress was examined by labelling with [35S]methionine. An increase in culture temperature from 26 degrees C to 37 degrees C induced expression of certain bacterial proteins (70 and 60 kDa). Heat shock at 40 degrees C, cold shock (10 degrees C), ethanol treatment or arsenite treatment also led to an increased expression of heat shock proteins of 70 and 60 kDa. Actinomycin D completely blocked the induction, indicating that transcription is required for the overexpression of stress proteins in Leuconostoc mesenteroides. N-terminal sequence analysis showed that these proteins were homologous to the highly conserved chaperone proteins DnaK and GroEL of Escherichia coli, respectively.

Related Organizations

Jawaharlal Nehru University
India
Jawaharlal Nehru University
India

Keywords

Hot Temperature, Ethanol, Sequence Homology, Amino Acid, Arsenites, Escherichia coli Proteins, Sulfhydryl Reagents, Chaperonin 60, Sodium Compounds, Cold Temperature, Molecular Weight, Bacterial Proteins, HSP70 Heat-Shock Proteins, Leuconostoc

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	citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	45
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	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Top 10%
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