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Overcoming Biomass Recalcitrance by Combining Genetically Modified Switchgrass and Cellulose Solvent-Based Lignocellulose Pretreatment

descriptionPublicationkeyboard_double_arrow_right Article , Other literature type , Journal 27 Sep 2013 United States Publisher:Public Library of Science (PLoS)Journal:PLoS ONE, volume 8, page e73523 (eissn: 1932-6203,

Authors: Sathitsuksanoh, Noppadon; Xu, Bin; Zhao, Bingyu Y.; Zhang, Y. H. Percival;

doi: 10.1371/journal.pone.0073523

pmid: 24086283

pmc: PMC3785476

handle: 10919/56677

Overcoming Biomass Recalcitrance by Combining Genetically Modified Switchgrass and Cellulose Solvent-Based Lignocellulose Pretreatment

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Abstract

Decreasing lignin content of plant biomass by genetic engineering is believed to mitigate biomass recalcitrance and improve saccharification efficiency of plant biomass. In this study, we compared two different pretreatment methods (i.e., dilute acid and cellulose solvent) on transgenic plant biomass samples having different lignin contents and investigated biomass saccharification efficiency. Without pretreatment, no correlation was observed between lignin contents of plant biomass and saccharification efficiency. After dilute acid pretreatment, a strong negative correlation between lignin content of plant samples and overall glucose release was observed, wherein the highest overall enzymatic glucan digestibility was 70% for the low-lignin sample. After cellulose solvent- and organic solvent-based lignocellulose fractionation pretreatment, there was no strong correlation between lignin contents and high saccharification efficiencies obtained (i.e., 80-90%). These results suggest that the importance of decreasing lignin content in plant biomass to saccharification was largely dependent on pretreatment choice and conditions.

Country

United States

Related Organizations

Virginia Tech
United States

Keywords

Fermentable sugar yields, Dilute-acid pretreatment, Science, Biofuel production, Saccharification, Poaceae, Lignin, Fractionation, Biomass, Corn stover, Cellulose, Ethanol, Lignin modification, Enzymatic-hydrolysis, Hydrolysis, Q, R, Accessibility, Plants, Genetically Modified, Microscopy, Fluorescence, Medicine, Research Article

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