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Ultrasonic synthetic technique to manufacture a pHEMA nanopolymeric-based vaccine against the H6N2 avian influenza virus: a preliminary investigation

descriptionPublicationkeyboard_double_arrow_right Article , Other literature type , Journal 01 Sep 2011 Australia Publisher:Informa UK LimitedJournal:International Journal of Nanomedicine (eissn: 1178-2013,

Authors: Poinern, G.E.J.; Le, X.T.; Shan, S.; Ellis, T.; Fenwick, S.; Edwards, J.; Fawcett, D.;

doi: 10.2147/ijn.s24272

pmid: 22114480

pmc: PMC3215157

Ultrasonic synthetic technique to manufacture a pHEMA nanopolymeric-based vaccine against the H6N2 avian influenza virus: a preliminary investigation

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Abstract

This preliminary study investigated the use of poly (2-hydroxyethyl methacrylate) (pHEMA) nanoparticles for the delivery of the deoxyribonucleic acid (DNA) vaccine pCAG-HAk, which expresses the full length hemagglutinin (HA) gene of the avian influenza A/Eurasian coot/Western Australian/2727/1979 (H6N2) virus with a Kozak sequence which is in the form of a pCAGGS vector. The loaded and unloaded nanoparticles were characterized using field-emission scanning electron microscopy. Further characterizations of the nanoparticles were made using atomic force microscopy and dynamic light scattering, which was used to investigate particle size distributions. This preliminary study suggests that using 100 μg of pHEMA nanoparticles as a nanocarrier/adjuvant produced a reduction in virus shedding and improved the immune response to the DNA vaccine pCAG-HAk.

Country

Australia

Related Organizations

Australian Animal Health Laboratory
Australia
Murdoch University
Australia
Australian Animal Health Laboratory
Australia
Commonwealth Scientific and Industrial Research Organisation
Australia
Murdoch University
Australia

Keywords

Medicine (General), Hemagglutinin Glycoproteins, Influenza Virus, Sonication, R5-920, International Journal of Nanomedicine, Vaccines, DNA, Animals, Particle Size, Polyhydroxyethyl Methacrylate, Original Research, Drug Carriers, Ethanol, DNA, Virus Shedding, Influenza A virus, Influenza Vaccines, Influenza in Birds, Nanoparticles, Chickens, Plasmids

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	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Average
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Top 10%