The concept of Industry 4.0 has been mainly addressed by the current literature from a technological perspective, overlooking the organisational and even ethical challenges related to this recent paradigm. In order to become ‘4.0 compliant’, an enterprise must adapt its organisation and business approaches, and these changes may lead to a significant impact on sustainability. Therefore, we performed a systematic literature review to investigate the most recent Industry 4.0 research streams by adopting a multi-perspective approach. This analysis led to collect insights on the key traits of an Enterprise 4.0: integration, decomposed hierarchy, flexibility, and autonomy. Each of these keywords involves work environments, business and organisational models, and educational approaches, which constitute the key traits of the novel framework proposed in this study.