A preliminary study was conducted to analyze the sustainability of barley production through: (i) investigating sensor-based nitrogen (N) application on barley performance, compared with conventional N management (CT); (ii) assessing the potential of the Normalized Difference Vegetation Index (NDVI) at different growth stages for within-season predictions of crop parameters; and (iii) evaluating sensor-based fertilization benefits in the form of greenhouse gasses mitigation. Barley was grown under CT, sensor-based management (RF) and with no N fertilization (Control). NDVI measurements and RF fertilization were performed using a GreenSeeker™ 505 hand-held optical sensor. Gas emissions were measured using a static chamber method with a portable gas analyzer. Results showed that barley yield was not statistically different under RF and CF, while they both differed significantly from Control. Highly significant positive correlations were observed between NDVI and production parameters at harvesting from the middle of stem elongation to the medium milk stage across treatments. Our findings suggest that RF is able to decrease CO2 emission in comparison with CF. The relationship between N fertilization and CH4 emission showed high variability. These preliminary results provide an indication of the benefits achieved using a simple proximal sensing methodology to support N fertilization.