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Cholesterol Reduction and Vitamin B12 Production Study on Enterococcus faecium and Lactobacillus pentosus Isolated from Yoghurt

descriptionPublicationkeyboard_double_arrow_right Article , Journal , Other literature type 23 May 2021Publisher:MDPI AGJournal:Sustainability, volume 13, page 5,853 (eissn: 2071-1050,

Authors: Rajan A. Walhe; Sham S. Diwanay; Milind S. Patole; R. Z. Sayyed; Hind A. AL-Shwaiman; Manal M. Alkhulaifi; Abdallah M. Elgorban; +2 Authors

doi: 10.3390/su13115853

Cholesterol Reduction and Vitamin B12 Production Study on Enterococcus faecium and Lactobacillus pentosus Isolated from Yoghurt

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Abstract

The present study was aimed to test cholesterol reduction and vitamin B12 production abilities of the isolated lactic acid bacteria (LAB). Three LAB isolates, namely, Enterococcus faecium (EF), Enterococcus faecium (Chole1), and Lactobacillus pentosus (7MP), having probiotic potential, were isolated from yoghurt. These isolates were screened for bile salt hydrolase (BSH) activity, cholesterol reduction property in MRS broth, and the production of vitamin B12. The present study revealed that the isolate 7MP possesses the highest potential of (48%) cholesterol reduction compared to the other isolates. The isolates EF and Chole1 produced a good amount of (1 ng/mL) vitamin B12. These isolates were identified by 16S rRNA gene sequencing and confirmed by MALD_TOF analysis. Thus, the use of these LAB isolates for yoghurt-making can offer the value addition of lowering cholesterol and vitamin B12 fortification in fermented food.

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Keywords

Environmental effects of industries and plants, TJ807-830, TD194-195, Renewable energy sources, cholesterol reduction, vitamin B12 production, Environmental sciences, Enterococcus faecium, GE1-350, yoghurt, Lactobacillus pentosus

Impact byBIP!

	citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	12
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Top 10%
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Average
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Top 10%