The aim of this study was to determine the ecological status of fish fauna of Razim Lake under the conditions of the water salinity changing from brackish, almost 70 years ago, into freshwater nowadays. The natural processes of siltation and organic deposits, characteristic of Danube Delta lake complexes, intensified in the last decades and included also Razim Lake. The presently reported study of Razim Lake and the adjacent area was undertaken in 2020 with intention to cover fish fauna collected with three different sampling methods (electrofishing, gillnetting, and seining). For each sampling method, Catch per Unit Effort (CPUE), relative abundance, and biomass were determined, as well as selected ecological parameters to determine ecological status of richness species in the area. Published data included 55 fish species, mainly marine and euryhaline, but in 2020 only 43 species were reported. Also, the species composition shifted from marine ones to freshwater or euryhaline ones. Of those 43 species captured in 2020 from Razim Lake and neighboring areas, 39 were native and four were non-native, including a newcomer, the Chinese sleeper, Perccottus glenii Dybowski, 1877. Few species were migratory, reophilous, or reophilous-stagnophilous which rarely enter Razim Lake, but the majority were limnophilous or stagnophilous-reophilous species. Four species were dominant in terms of the abundance; Blicca bjoerkna (Linnaeus, 1758); Rutilus rutilus (Linnaeus, 1758); Alburnus alburnus (Linnaeus, 1758); and Carassius gibelio (Bloch, 1782). In terms of the biomass the dominants were: Cyprinus carpio Linnaeus, 1758; Carassius gibelio; Sander lucioperca (Linnaeus, 1758); Pelecus cultratus (Linnaeus, 1758); and Blicca bjoerkna. Some differences between sampling methods used were observed. Eudominant, euconstant, and main species were Blicca bjoerkna and the majority of fish species were accessories, with differences amongst sampling methods used. Fish diversity parameters indicate a stable ichthyocoenosis, more stable along the lake shoreline. Ecological indicators of fish fauna from Razim Lake in 2020 grade the water lake quality as a moderate ecological class according to the Water Framework Directive of the European Union.