Human plasma albumin was incubated in 25 mM/l glucose at 37 degrees C for up to sevent days. Aliquots of this mixture were also incubated with alpha-tocopherol (Vitamin E) at 2 mg/dl, 4 mg/dl and 8 mg/dl, respectively. Vitamin E inhibited protein glycation and, furthermore, shows a dose-dependent effect. This report suggests the possibility of using Vitamin E, at therapeutic doses, to obtain the inhibition of non-enzymatic glycation.