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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Abdul Ghaffar Shar; Jiao Ya Peng; Xiaohong Tian; Tanveer Ahmed Siyal; +4 Authors

    Mismanagement of crop straw and coal gas residue threatens the atmosphere and the economy. Nevertheless, thermal-pyrolysis is an option for management that turns bio-waste into biochar; its viability and adoption by the public as soil amendments is dependent on the agronomic and environmental values compared between biochar and the raw materials. We undertook a 60-day short-term analysis to assess the impact of various wastes and biochars, as well as inorganic nutrients (N), on carbon dioxide (CO2) fluxes, soil enzyme activities, soil fertility status, and microbial activities. There were eight treatments of soil amendments: without an amendment (CK), Nutrients (N), straw + nutrients (S+N), straw biochar + nutrients (SB+N), coal gas residue + nutrients (C+N), coal gas residue biochar + nutrients (CB+N), straw + straw biochar + nutrients (S+SB+N) and coal gas residue waste + coal gas residue biochar + nutrients (C+ CB +N). The results indicated that soil EC, pH, nitrate N (NO3 -- N), SOC, TN and available K were significantly (p < 0.05) increased coal gas residue biochar and combined with coal fly ash as compared to maize straw biochar and combined with maize straw and N treatments. The higher concentrations of soil MBC and MBN activities were increased in the maize straw application, while higher soil enzyme activity such as, invertase, urease and catalase were enhanced in the coal fly ash derived biochar treatments. The higher cumulative CO2 emissions were recorded in the combined applications of maize straw and its biochar as well as coal gas residue and its biochar treatment. Our study concludes, that maize straw and coal fly ash wastes were converted into biochar product could be a feasible substitute way of discarding, since land amendment and decreased CO2 fluxes and positive changes in soil microbial, and chemical properties, and can be confirmed under long-term conditions for reduction of economical and environment issues.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Saudi Journal of Bio...arrow_drop_down
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Saudi Journal of Biological Sciences
    Article . 2021 . Peer-reviewed
    License: CC BY NC ND
    Data sources: Crossref
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Saudi Journal of Biological Sciences
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    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Saudi Journal of Bio...arrow_drop_down
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      Saudi Journal of Biological Sciences
      Article . 2021 . Peer-reviewed
      License: CC BY NC ND
      Data sources: Crossref
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      Saudi Journal of Biological Sciences
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Asif Ullah Khan; Faizan Ullah; Naeem Khan; Sultan Mehmood; +11 Authors

    Linseed is an important industrial crop cultivated for its edible seeds and fiber linen. Organic fertilizers have beneficial effects on soil properties and quality of crops. Therefore, we conducted two field experiments during 2018–2019 and 2019–2020 to determine the effect of organic fertilizers on soil fertility, yield and fiber quality of linseed varieties Roshni, BL1 and Chandni under low soil moisture conditions. We prepared organic fertilizers from seed cake of Eruca sativa, leaves of Moringa oleifera and chicken peat in various combinations by composting method. The various formulations of organic fertilizers included OF1(1 kg seed cake of Eruca sativa), OF2 (1 kg seed cake of Eruca sativa + 1 kg chicken peat), OF3 (1 kg seed cake of Eruca sativa + 0.5 kg chicken peat + 0.25 kg Moringa oliefera leaves) and OF4 (1 kg seed cake of Eruca sativa + 0.250 kg chicken peat + 0.5 kg Moringa oliefera leaves). Compositional analysis of organic fertilizers indicated that OF3 and OF4 had higher and may potentially sufficient quantities of NPK and organic matter. Both of these fertilizers significantly improved soil total N, available P, K, Zn and Fe contents. Growth response of linseed varieties to organic fertilizers was evaluated under water deficit stress (40% field capacity of soil) at tillering stage for one month. Water stress had significantly adverse effects on plant height, production of tillers per plant, leaf relative water content (LRWC), number of capsules per plant, thousand seed weight, total seed yield, straw yield, fiber length and fiber weight of linseed varieties. However, the application of OF3 and OF4 significantly enhanced plant height, tillers production, LRWC, seed yield, straw yield, fiber length and fiber weight under water deficit stress. Water deficit stress also resulted in a significant increase in the content of phenolics of both the leaves and roots. For each measured quality parameter of linseed varieties, organic fertilizer treatments resulted in higher values than untreated and irrigated control. We concluded that organic fertilizers particularly OF3 and OF4 significantly improved soil fertility and minimized negative effect of water deficit stress on plant height, tillers production, LRWC, seed yield, straw yield, fiber length and fiber weight of linseed varieties.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Sustainabilityarrow_drop_down
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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    Article . 2020 . Peer-reviewed
    License: CC BY
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    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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    Article . 2020
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    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Sustainabilityarrow_drop_down
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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      Article . 2020 . Peer-reviewed
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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      Article . 2020
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Khadim Dawar; Zeeshan Khalil; Ishaq Ahmad Mian; Bushra Khan; +9 Authors

    Less phosphorus (P) availability in calcareous alkaline soils is one of the major problems in achieving an optimum crop yield. Its deficiency in plants adversely affects growth and yield attributes. To overcome this issue, growers incorporate inorganic P fertilizers. However, the need for time in the sustainable management of soil fertility in terms of P. Farmyard manure (FYM) application is one of the most popular organic amendments in this regard. Thus, the current study was conducted to explore the best application rate of FYM in combination with inorganic P fertilizer single super phosphate (SSP). There were six treatments i.e., control (0F), 100%SSP (100P), 25% FYM and 75% SSP (25F+75P), 50% FYM and 50% SSP (50F+50P), 75% FYM and 25% SSP (75F+25P), and 100% FYM (100F+0P), applied in three replications. The design of the experiment was a randomized complete block design. For assessment of treatment response, two wheat cultivars (V1 = Pirsabak and V2 = Atta Habib) were used. Results showed that the application of 50F+50P significantly improved the plant height (20.69 and 32.01%), spike/m2 (35.19 and 30.10%), grain (41.10 and 38.16%), and leaf P (49.82 and 71.32%) compared to control in V1 and V2, respectively. A significant improvement in the grain and the biological yield of wheat V2 also validated the efficacious functioning of 50F+50P over control. In conclusion, 50F+50P has the potential to enhance wheat growth and nutrient concentration over control. More investigations are required for a more precise and balanced synchronization of FYM and SSP for the achievement of maximum wheat yield.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Sustainabilityarrow_drop_down
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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    Article . 2022 . Peer-reviewed
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    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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    Article . 2022
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Sustainabilityarrow_drop_down
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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      Article . 2022 . Peer-reviewed
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Mohammad Rafiqul Islam; Sultana Bilkis; Tahsina Sharmin Hoque; Shihab Uddin; +7 Authors

    A good understanding of nutrient release from manure or compost after application through mineralization is important to assure meeting the nutrient demand of crops, to secure timely fertilizer application and to enhance nutrient use efficiency. The current study was done to evaluate phosphorus (P) and sulphur (S) release patterns from different types of manures viz. cow dung, cow dung slurry, tricho-compost, vermicompost, poultry manure, poultry manure slurry and mungbean residues. The mineralization study was performed under aerobic (field capacity) and anaerobic (waterlogging) conditions for 180 days at 25 ± 1 °C in the laboratory. The release of P and S showed the highest values within 75–180 and 75–150 days, respectively, and was always higher in aerobic conditions than in anaerobic conditions. The first-order kinetic cumulative model was a good fit for mineralization, which was significantly influenced by manure type, soil moisture level and incubation period. Poultry manure slurry exerted the highest P and S release under both moisture conditions. Both slurries showed higher potential mineralization, with a lower rate constant for these elements compared to that in their manure states. Hence, appropriate manures should be chosen and applied in the proper quantity to provide exact amounts of nutrients, to increase crops nutrient use efficiency and to formulate correct fertilizer recommendations.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Sustainabilityarrow_drop_down
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    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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    Article . 2021 . Peer-reviewed
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    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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    Article . 2021
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    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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    Authors: Martin Brtnicky; Tereza Dokulilova; Jiri Holatko; Vaclav Pecina; +5 Authors

    Biochar application to the soil has been recommended as a carbon (C) management approach to sequester C and improve soil quality. Three-year experiments were conducted to investigate the interactive effects of three types of amendments on microbial biomass carbon, soil dehydrogenase activity and soil microbial community abundance in luvisols of arable land in the Czech Republic. Four different treatments were studied, which were, only NPK as a control, NPK + cattle manure, NPK + biochar and NPK + combination of manure with biochar. The results demonstrate that all amendments were effective in increasing the fungal and bacterial biomass, as is evident from the increased values of bacterial and fungal phospholipid fatty acid analysis. The ammonia-oxidizing bacteria population increases with the application of biochar, and it reaches its maximum value when biochar is applied in combination with manure. The overall results suggest that co-application of biochar with manure changes soil properties in favor of increased microbial biomass. It was confirmed that the application of biochar might increase or decrease soil activity, but its addition, along with manure, always promotes microbial abundance and their activity. The obtained results can be used in the planning and execution of the biochar-based soil amendments.

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    Authors: Muhammad Zafar-ul-Hye; Muhammad Naeem Akbar; Yasir Iftikhar; Mazhar Abbas; +7 Authors

    Lentil (Lens culinaris Medik) is an important component of the human diet due to its high mineral and protein contents. Abiotic stresses, i.e., drought, decreases plant growth and yield. Drought causes the synthesis of reactive oxygen species, which decrease a plant’s starch contents and growth. However, ACC-deaminase (1-aminocyclopropane-1-carboxylate deaminase) producing rhizobacteria can alleviate drought stress by decreasing ethylene levels. On the other hand, caffeic acid (CA) can also positively affect cell expansion and turgor pressure maintenance under drought stress. Therefore, the current study was planned with an aim to assess the effect of CA (0, 20, 50 and 100 ppm) and ACC-deaminase rhizobacteria (Lysinibacillus fusiform, Bacillus amyloliquefaciens) on lentils under drought stress. The combined application of CA and ACC-deaminase containing rhizobacteria significantly improved plant height (55%), number of pods per plant (51%), 1000-grain weight (45%), nitrogen concentration (56%), phosphorus concentration (19%), potassium concentration (21%), chlorophyll (54%), relative water contents RWC (60%) and protein contents (55%). A significant decrease in electrolyte leakage (30%), proline contents (44%), and hydrogen peroxide contents (54%), along with an improvement in cell membrane stability (34% over control) validated the combined use of CA and rhizobacteria. In conclusion, co-application of CA (20 ppm) and ACC-deaminase producing rhizobacteria can significantly improve plant growth and yield for farmers under drought stress. More investigations are suggested at the field level to select the best rhizobacteria and CA level for lentils under drought.

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    Authors: Eliska Kobzova; Antonin Kintl; Rahul Datta; Tereza Hammerschmiedt; +12 Authors

    Objectives As a liquid organic fertilizer used in agriculture, digestate is rich in many nutrients (i.e. nitrogen, phosphorus, sulfur, calcium, potassium); their utilization may be however less efficient in soils poor in organic carbon (due to low carbon:nitrogen ratio). In order to solve the disadvantages, digestate enrichment with carbon-rich amendments biochar or humic acids (Humac) was tested. Methods Soil variants amended with enriched digestate: digestate + biochar, digestate + Humac, and digestate + combined biochar and humic acids—were compared to control with untreated digestate in their effect on total soil carbon and nitrogen, microbial biomass carbon, soil respiration and soil enzymatic activities in a pot experiment. Yield of the test crop lettuce was also determined for all variants. Results Soil respiration was the most significantly increased property, positively affected by digestate + Humac. Both digestate + biochar and digestate + Humac significantly increased microbial biomass carbon. Significant negative effect of digestate + biochar (compared to the control digestate) on particular enzyme activities was alleviated by the addition of humic acids. No significant differences among the tested variants were found in the above-ground and root plant biomass. Conclusions The tested organic supplements improved the digestate effect on some determined soil properties. We deduced from the results (carbon:nitrogen ratio, microbial biomass and activity) that the assimilation of nutrients by plants increased; however, the most desired positive effect on the yield of crop biomass was not demonstrated. We assume that the digestate enrichment with organic amendments may be more beneficial in a long time-scaled trial.

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    Authors: Ni Luh Suriani; Dewa Ngurah Suprapta; Agung Wiwiek Indrayani; Susila Herlambang; +7 Authors

    Bali is a world tourist destination and has many natural resources that need to be developed to support the tourism sector. One of the local Bali resources that has the potential to be developed to support tourism and food is the local red Bali rice. This local Balinese rice is a characteristic of the ecotourism area of the Jatiluwih village of Tabanan, Bali. Balinese rice is grown with inorganic pesticides and there is an urgent need to develop organic pesticides as a sustainable approach to rice farming. In this regard, extracts of piper plants can serve as the best and greenest biopesticides as plant growth-promoting rhizobacteria (PGPR), and compost functions as organic fertilizer. The present research aimed to evaluate PGPR, compost, and the synergistic biopesticidal effects of extracts of three piper plants, namely Piper caninum, Piper betle var. Nigra, and Piper betle, against blast disease in Bali red rice plants. The results showed that the synergistic action of PGPR, compost, and crude extract of piper plant provided an inhibitory activity against blast disease in rice plants where the greatest inhibition was found in a mixture of the three extracts with an inhibition of 50 cm. This shows that the mixed compounds of the three piper extracts work synergistically in suppressing blast disease; in addition, PGPR also exhibited a positive impact on the growth of red rice because PGPR produce growth hormones and various antifungal metabolites that help the plant growth and induce systemic resistance against phytopathogens. The active principles were identified as citronella, trans-geraniol, and 4.6-dipropyl-nonan-5-one. A combination of these extracts with compost and PGPR showed potential antifungal activity against blast disease at a concentration of 2%. This application also promoted the growth of Bali red rice. There is a significant increase in the number of leaves and the number of tillers, where the height is inversely proportional to the higher the extract up to 2%, as the height of the red Bali rice plant decreases. This is good because it reduces the red Bali rice stalks’ possibility of falling during small production. The piper extract mixture at a concentration of 2% had the highest effect on grain production/tonne (6.59 tonne/ha) compared to the control at only 3.21–3.41 tonnes/ ha. The 2% concentration of the extracts from the mixture of the three pipers has the highest effect on growth and red Bali rice production, and provides the greatest obstacle to the intensity of blast disease in red Bali rice.

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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Zarmeena Khan; Muhammad Habib ur Rahman; Ghulam Haider; Rabia Amir; +10 Authors

    Nitrogen (N) losses are prevalent under South East Asia’s due to high N fertilizer inputs, but low N fertilizer use efficiency. This leaves a large quantity of reactive N at risk of loss to the environment. Biochar has been found to reduce N losses across a variety of soil types, however, there is limited data available for semi-arid climates, particularly at a field-scale. Herein we present an exploration of the biological and chemical enhancement effects observed of a cotton stalk-based biochar on wheat growth and yield under arid field conditions. The biochar was treated with urea-N and biofertilizer (bio-power) in different treatment setups. The six experimental treatments included; (i) a full N dose “recommended for wheat crops in the region” (104 kg N ha−1) as a positive control; (ii) a half N dose (52 kg N ha−1); (iii) a half N dose + biofertilizer (4.94 kg ha−1) as a soil mixture; (iv) a half N dose + biofertilizer as a seed inoculation; (v) a full N dose as broadcast + biochar (5 t ha−1) inoculated with biofertilizer; and (vi) a full N dose loaded on biochar + biofertilizer applied as a soil mixture. The half dose N application or biofertilizer addition as soil mix/seed inoculated/biochar inoculation with biofertilizer caused reduced wheat growth and yield compared to the control (conventional N fertilization). However, co-application of chemically enhanced biochar (loaded with a full N dose) and biofertilizer as soil mixture significantly increased the crop growth rate (CGR) and leaf area index (LAI). A significantly higher crop growth and canopy development led to a higher light interception and radiation use efficiency (RUE) for total dry matter (TDM) and grain yield (11% greater than control) production compared to the control. A greater grain yield, observed for the full N dose loaded on biochar + biofertilizer applied as a soil mixture, is attributed to prolonged N availability as indicated by greater plant and soil N content at harvest and different crop growth stages, respectively. The present study has improved our understanding of how the application of nitrogen loaded biochar and biofertilizer as soil mixtures can synergize to positively affect wheat growth and soil-nitrogen retention under arid environmental conditions.

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    Authors: Rajan A. Walhe; Sham S. Diwanay; Milind S. Patole; R. Z. Sayyed; +5 Authors

    The present study was aimed to test cholesterol reduction and vitamin B12 production abilities of the isolated lactic acid bacteria (LAB). Three LAB isolates, namely, Enterococcus faecium (EF), Enterococcus faecium (Chole1), and Lactobacillus pentosus (7MP), having probiotic potential, were isolated from yoghurt. These isolates were screened for bile salt hydrolase (BSH) activity, cholesterol reduction property in MRS broth, and the production of vitamin B12. The present study revealed that the isolate 7MP possesses the highest potential of (48%) cholesterol reduction compared to the other isolates. The isolates EF and Chole1 produced a good amount of (1 ng/mL) vitamin B12. These isolates were identified by 16S rRNA gene sequencing and confirmed by MALD_TOF analysis. Thus, the use of these LAB isolates for yoghurt-making can offer the value addition of lowering cholesterol and vitamin B12 fortification in fermented food.

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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Abdul Ghaffar Shar; Jiao Ya Peng; Xiaohong Tian; Tanveer Ahmed Siyal; +4 Authors

    Mismanagement of crop straw and coal gas residue threatens the atmosphere and the economy. Nevertheless, thermal-pyrolysis is an option for management that turns bio-waste into biochar; its viability and adoption by the public as soil amendments is dependent on the agronomic and environmental values compared between biochar and the raw materials. We undertook a 60-day short-term analysis to assess the impact of various wastes and biochars, as well as inorganic nutrients (N), on carbon dioxide (CO2) fluxes, soil enzyme activities, soil fertility status, and microbial activities. There were eight treatments of soil amendments: without an amendment (CK), Nutrients (N), straw + nutrients (S+N), straw biochar + nutrients (SB+N), coal gas residue + nutrients (C+N), coal gas residue biochar + nutrients (CB+N), straw + straw biochar + nutrients (S+SB+N) and coal gas residue waste + coal gas residue biochar + nutrients (C+ CB +N). The results indicated that soil EC, pH, nitrate N (NO3 -- N), SOC, TN and available K were significantly (p < 0.05) increased coal gas residue biochar and combined with coal fly ash as compared to maize straw biochar and combined with maize straw and N treatments. The higher concentrations of soil MBC and MBN activities were increased in the maize straw application, while higher soil enzyme activity such as, invertase, urease and catalase were enhanced in the coal fly ash derived biochar treatments. The higher cumulative CO2 emissions were recorded in the combined applications of maize straw and its biochar as well as coal gas residue and its biochar treatment. Our study concludes, that maize straw and coal fly ash wastes were converted into biochar product could be a feasible substitute way of discarding, since land amendment and decreased CO2 fluxes and positive changes in soil microbial, and chemical properties, and can be confirmed under long-term conditions for reduction of economical and environment issues.

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    Saudi Journal of Biological Sciences
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      Saudi Journal of Biological Sciences
      Article . 2021 . Peer-reviewed
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    Authors: Asif Ullah Khan; Faizan Ullah; Naeem Khan; Sultan Mehmood; +11 Authors

    Linseed is an important industrial crop cultivated for its edible seeds and fiber linen. Organic fertilizers have beneficial effects on soil properties and quality of crops. Therefore, we conducted two field experiments during 2018–2019 and 2019–2020 to determine the effect of organic fertilizers on soil fertility, yield and fiber quality of linseed varieties Roshni, BL1 and Chandni under low soil moisture conditions. We prepared organic fertilizers from seed cake of Eruca sativa, leaves of Moringa oleifera and chicken peat in various combinations by composting method. The various formulations of organic fertilizers included OF1(1 kg seed cake of Eruca sativa), OF2 (1 kg seed cake of Eruca sativa + 1 kg chicken peat), OF3 (1 kg seed cake of Eruca sativa + 0.5 kg chicken peat + 0.25 kg Moringa oliefera leaves) and OF4 (1 kg seed cake of Eruca sativa + 0.250 kg chicken peat + 0.5 kg Moringa oliefera leaves). Compositional analysis of organic fertilizers indicated that OF3 and OF4 had higher and may potentially sufficient quantities of NPK and organic matter. Both of these fertilizers significantly improved soil total N, available P, K, Zn and Fe contents. Growth response of linseed varieties to organic fertilizers was evaluated under water deficit stress (40% field capacity of soil) at tillering stage for one month. Water stress had significantly adverse effects on plant height, production of tillers per plant, leaf relative water content (LRWC), number of capsules per plant, thousand seed weight, total seed yield, straw yield, fiber length and fiber weight of linseed varieties. However, the application of OF3 and OF4 significantly enhanced plant height, tillers production, LRWC, seed yield, straw yield, fiber length and fiber weight under water deficit stress. Water deficit stress also resulted in a significant increase in the content of phenolics of both the leaves and roots. For each measured quality parameter of linseed varieties, organic fertilizer treatments resulted in higher values than untreated and irrigated control. We concluded that organic fertilizers particularly OF3 and OF4 significantly improved soil fertility and minimized negative effect of water deficit stress on plant height, tillers production, LRWC, seed yield, straw yield, fiber length and fiber weight of linseed varieties.

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    Authors: Khadim Dawar; Zeeshan Khalil; Ishaq Ahmad Mian; Bushra Khan; +9 Authors

    Less phosphorus (P) availability in calcareous alkaline soils is one of the major problems in achieving an optimum crop yield. Its deficiency in plants adversely affects growth and yield attributes. To overcome this issue, growers incorporate inorganic P fertilizers. However, the need for time in the sustainable management of soil fertility in terms of P. Farmyard manure (FYM) application is one of the most popular organic amendments in this regard. Thus, the current study was conducted to explore the best application rate of FYM in combination with inorganic P fertilizer single super phosphate (SSP). There were six treatments i.e., control (0F), 100%SSP (100P), 25% FYM and 75% SSP (25F+75P), 50% FYM and 50% SSP (50F+50P), 75% FYM and 25% SSP (75F+25P), and 100% FYM (100F+0P), applied in three replications. The design of the experiment was a randomized complete block design. For assessment of treatment response, two wheat cultivars (V1 = Pirsabak and V2 = Atta Habib) were used. Results showed that the application of 50F+50P significantly improved the plant height (20.69 and 32.01%), spike/m2 (35.19 and 30.10%), grain (41.10 and 38.16%), and leaf P (49.82 and 71.32%) compared to control in V1 and V2, respectively. A significant improvement in the grain and the biological yield of wheat V2 also validated the efficacious functioning of 50F+50P over control. In conclusion, 50F+50P has the potential to enhance wheat growth and nutrient concentration over control. More investigations are required for a more precise and balanced synchronization of FYM and SSP for the achievement of maximum wheat yield.

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    Authors: Mohammad Rafiqul Islam; Sultana Bilkis; Tahsina Sharmin Hoque; Shihab Uddin; +7 Authors

    A good understanding of nutrient release from manure or compost after application through mineralization is important to assure meeting the nutrient demand of crops, to secure timely fertilizer application and to enhance nutrient use efficiency. The current study was done to evaluate phosphorus (P) and sulphur (S) release patterns from different types of manures viz. cow dung, cow dung slurry, tricho-compost, vermicompost, poultry manure, poultry manure slurry and mungbean residues. The mineralization study was performed under aerobic (field capacity) and anaerobic (waterlogging) conditions for 180 days at 25 ± 1 °C in the laboratory. The release of P and S showed the highest values within 75–180 and 75–150 days, respectively, and was always higher in aerobic conditions than in anaerobic conditions. The first-order kinetic cumulative model was a good fit for mineralization, which was significantly influenced by manure type, soil moisture level and incubation period. Poultry manure slurry exerted the highest P and S release under both moisture conditions. Both slurries showed higher potential mineralization, with a lower rate constant for these elements compared to that in their manure states. Hence, appropriate manures should be chosen and applied in the proper quantity to provide exact amounts of nutrients, to increase crops nutrient use efficiency and to formulate correct fertilizer recommendations.

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    Authors: Martin Brtnicky; Tereza Dokulilova; Jiri Holatko; Vaclav Pecina; +5 Authors

    Biochar application to the soil has been recommended as a carbon (C) management approach to sequester C and improve soil quality. Three-year experiments were conducted to investigate the interactive effects of three types of amendments on microbial biomass carbon, soil dehydrogenase activity and soil microbial community abundance in luvisols of arable land in the Czech Republic. Four different treatments were studied, which were, only NPK as a control, NPK + cattle manure, NPK + biochar and NPK + combination of manure with biochar. The results demonstrate that all amendments were effective in increasing the fungal and bacterial biomass, as is evident from the increased values of bacterial and fungal phospholipid fatty acid analysis. The ammonia-oxidizing bacteria population increases with the application of biochar, and it reaches its maximum value when biochar is applied in combination with manure. The overall results suggest that co-application of biochar with manure changes soil properties in favor of increased microbial biomass. It was confirmed that the application of biochar might increase or decrease soil activity, but its addition, along with manure, always promotes microbial abundance and their activity. The obtained results can be used in the planning and execution of the biochar-based soil amendments.

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    Authors: Muhammad Zafar-ul-Hye; Muhammad Naeem Akbar; Yasir Iftikhar; Mazhar Abbas; +7 Authors

    Lentil (Lens culinaris Medik) is an important component of the human diet due to its high mineral and protein contents. Abiotic stresses, i.e., drought, decreases plant growth and yield. Drought causes the synthesis of reactive oxygen species, which decrease a plant’s starch contents and growth. However, ACC-deaminase (1-aminocyclopropane-1-carboxylate deaminase) producing rhizobacteria can alleviate drought stress by decreasing ethylene levels. On the other hand, caffeic acid (CA) can also positively affect cell expansion and turgor pressure maintenance under drought stress. Therefore, the current study was planned with an aim to assess the effect of CA (0, 20, 50 and 100 ppm) and ACC-deaminase rhizobacteria (Lysinibacillus fusiform, Bacillus amyloliquefaciens) on lentils under drought stress. The combined application of CA and ACC-deaminase containing rhizobacteria significantly improved plant height (55%), number of pods per plant (51%), 1000-grain weight (45%), nitrogen concentration (56%), phosphorus concentration (19%), potassium concentration (21%), chlorophyll (54%), relative water contents RWC (60%) and protein contents (55%). A significant decrease in electrolyte leakage (30%), proline contents (44%), and hydrogen peroxide contents (54%), along with an improvement in cell membrane stability (34% over control) validated the combined use of CA and rhizobacteria. In conclusion, co-application of CA (20 ppm) and ACC-deaminase producing rhizobacteria can significantly improve plant growth and yield for farmers under drought stress. More investigations are suggested at the field level to select the best rhizobacteria and CA level for lentils under drought.

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    Authors: Eliska Kobzova; Antonin Kintl; Rahul Datta; Tereza Hammerschmiedt; +12 Authors

    Objectives As a liquid organic fertilizer used in agriculture, digestate is rich in many nutrients (i.e. nitrogen, phosphorus, sulfur, calcium, potassium); their utilization may be however less efficient in soils poor in organic carbon (due to low carbon:nitrogen ratio). In order to solve the disadvantages, digestate enrichment with carbon-rich amendments biochar or humic acids (Humac) was tested. Methods Soil variants amended with enriched digestate: digestate + biochar, digestate + Humac, and digestate + combined biochar and humic acids—were compared to control with untreated digestate in their effect on total soil carbon and nitrogen, microbial biomass carbon, soil respiration and soil enzymatic activities in a pot experiment. Yield of the test crop lettuce was also determined for all variants. Results Soil respiration was the most significantly increased property, positively affected by digestate + Humac. Both digestate + biochar and digestate + Humac significantly increased microbial biomass carbon. Significant negative effect of digestate + biochar (compared to the control digestate) on particular enzyme activities was alleviated by the addition of humic acids. No significant differences among the tested variants were found in the above-ground and root plant biomass. Conclusions The tested organic supplements improved the digestate effect on some determined soil properties. We deduced from the results (carbon:nitrogen ratio, microbial biomass and activity) that the assimilation of nutrients by plants increased; however, the most desired positive effect on the yield of crop biomass was not demonstrated. We assume that the digestate enrichment with organic amendments may be more beneficial in a long time-scaled trial.

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    https://dx.doi.org/10.60692/79...
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    Authors: Ni Luh Suriani; Dewa Ngurah Suprapta; Agung Wiwiek Indrayani; Susila Herlambang; +7 Authors

    Bali is a world tourist destination and has many natural resources that need to be developed to support the tourism sector. One of the local Bali resources that has the potential to be developed to support tourism and food is the local red Bali rice. This local Balinese rice is a characteristic of the ecotourism area of the Jatiluwih village of Tabanan, Bali. Balinese rice is grown with inorganic pesticides and there is an urgent need to develop organic pesticides as a sustainable approach to rice farming. In this regard, extracts of piper plants can serve as the best and greenest biopesticides as plant growth-promoting rhizobacteria (PGPR), and compost functions as organic fertilizer. The present research aimed to evaluate PGPR, compost, and the synergistic biopesticidal effects of extracts of three piper plants, namely Piper caninum, Piper betle var. Nigra, and Piper betle, against blast disease in Bali red rice plants. The results showed that the synergistic action of PGPR, compost, and crude extract of piper plant provided an inhibitory activity against blast disease in rice plants where the greatest inhibition was found in a mixture of the three extracts with an inhibition of 50 cm. This shows that the mixed compounds of the three piper extracts work synergistically in suppressing blast disease; in addition, PGPR also exhibited a positive impact on the growth of red rice because PGPR produce growth hormones and various antifungal metabolites that help the plant growth and induce systemic resistance against phytopathogens. The active principles were identified as citronella, trans-geraniol, and 4.6-dipropyl-nonan-5-one. A combination of these extracts with compost and PGPR showed potential antifungal activity against blast disease at a concentration of 2%. This application also promoted the growth of Bali red rice. There is a significant increase in the number of leaves and the number of tillers, where the height is inversely proportional to the higher the extract up to 2%, as the height of the red Bali rice plant decreases. This is good because it reduces the red Bali rice stalks’ possibility of falling during small production. The piper extract mixture at a concentration of 2% had the highest effect on grain production/tonne (6.59 tonne/ha) compared to the control at only 3.21–3.41 tonnes/ ha. The 2% concentration of the extracts from the mixture of the three pipers has the highest effect on growth and red Bali rice production, and provides the greatest obstacle to the intensity of blast disease in red Bali rice.

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    Authors: Zarmeena Khan; Muhammad Habib ur Rahman; Ghulam Haider; Rabia Amir; +10 Authors

    Nitrogen (N) losses are prevalent under South East Asia’s due to high N fertilizer inputs, but low N fertilizer use efficiency. This leaves a large quantity of reactive N at risk of loss to the environment. Biochar has been found to reduce N losses across a variety of soil types, however, there is limited data available for semi-arid climates, particularly at a field-scale. Herein we present an exploration of the biological and chemical enhancement effects observed of a cotton stalk-based biochar on wheat growth and yield under arid field conditions. The biochar was treated with urea-N and biofertilizer (bio-power) in different treatment setups. The six experimental treatments included; (i) a full N dose “recommended for wheat crops in the region” (104 kg N ha−1) as a positive control; (ii) a half N dose (52 kg N ha−1); (iii) a half N dose + biofertilizer (4.94 kg ha−1) as a soil mixture; (iv) a half N dose + biofertilizer as a seed inoculation; (v) a full N dose as broadcast + biochar (5 t ha−1) inoculated with biofertilizer; and (vi) a full N dose loaded on biochar + biofertilizer applied as a soil mixture. The half dose N application or biofertilizer addition as soil mix/seed inoculated/biochar inoculation with biofertilizer caused reduced wheat growth and yield compared to the control (conventional N fertilization). However, co-application of chemically enhanced biochar (loaded with a full N dose) and biofertilizer as soil mixture significantly increased the crop growth rate (CGR) and leaf area index (LAI). A significantly higher crop growth and canopy development led to a higher light interception and radiation use efficiency (RUE) for total dry matter (TDM) and grain yield (11% greater than control) production compared to the control. A greater grain yield, observed for the full N dose loaded on biochar + biofertilizer applied as a soil mixture, is attributed to prolonged N availability as indicated by greater plant and soil N content at harvest and different crop growth stages, respectively. The present study has improved our understanding of how the application of nitrogen loaded biochar and biofertilizer as soil mixtures can synergize to positively affect wheat growth and soil-nitrogen retention under arid environmental conditions.

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    Authors: Rajan A. Walhe; Sham S. Diwanay; Milind S. Patole; R. Z. Sayyed; +5 Authors

    The present study was aimed to test cholesterol reduction and vitamin B12 production abilities of the isolated lactic acid bacteria (LAB). Three LAB isolates, namely, Enterococcus faecium (EF), Enterococcus faecium (Chole1), and Lactobacillus pentosus (7MP), having probiotic potential, were isolated from yoghurt. These isolates were screened for bile salt hydrolase (BSH) activity, cholesterol reduction property in MRS broth, and the production of vitamin B12. The present study revealed that the isolate 7MP possesses the highest potential of (48%) cholesterol reduction compared to the other isolates. The isolates EF and Chole1 produced a good amount of (1 ng/mL) vitamin B12. These isolates were identified by 16S rRNA gene sequencing and confirmed by MALD_TOF analysis. Thus, the use of these LAB isolates for yoghurt-making can offer the value addition of lowering cholesterol and vitamin B12 fortification in fermented food.

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