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description Publicationkeyboard_double_arrow_right Article , Journal 2014Publisher:Elsevier BV Authors:Riccardo Valentini;
Riccardo Valentini
Riccardo Valentini in OpenAIREMaurizio Ruzzi;
Maurizio Ruzzi
Maurizio Ruzzi in OpenAIREMaurizio Petruccioli;
Maurizio Petruccioli
Maurizio Petruccioli in OpenAIREAlessandro D’Annibale;
+3 AuthorsAlessandro D’Annibale
Alessandro D’Annibale in OpenAIRERiccardo Valentini;
Riccardo Valentini
Riccardo Valentini in OpenAIREMaurizio Ruzzi;
Maurizio Ruzzi
Maurizio Ruzzi in OpenAIREMaurizio Petruccioli;
Maurizio Petruccioli
Maurizio Petruccioli in OpenAIREAlessandro D’Annibale;
Alessandro D’Annibale
Alessandro D’Annibale in OpenAIREMauro Moresi;
Guglielmo Santi;Mauro Moresi
Mauro Moresi in OpenAIRESilvia Crognale;
Silvia Crognale
Silvia Crognale in OpenAIREOrange peel waste (OPW) was converted into ethanol by consecutive acid-catalysed steam-explosion (ACSE), enzymatic saccharification and fermentation with Saccharomyces cerevisiae F15. The first step was performed in the presence of 0.5% (volume fraction) sulphuric acid at a solid loading of 160 g L−1 using a novel laboratory-scale Direct Steam-Injection Apparatus under different reaction temperatures and times. ACSE at 200 °C for 90 s (or 180 °C for 150 s) yielded the highest solubilization of pectin (∼73%) and a positive effect on the subsequent enzymatic hydrolysis step, that was carried out at 50 °C with an enzyme loading of 6 FPU g−1 cellulose. OPW, pretreated at 180 °C for 150 s, yielded the highest glucose solubilisation degree (∼56%) at the end of the saccharification step, and the maximum ethanol yield coefficient (∼0.495 g g−1) and productivity (4.85 g L−1 h−1) at the end of the 3rd repeated fermentation batch in shaken-flasks.
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You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
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For further information contact us at helpdesk@openaire.eu48 citations 48 popularity Top 10% influence Top 10% impulse Top 10% Powered by BIP!
more_vert add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.biombioe.2013.12.007&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2014Publisher:Elsevier BV Authors:Riccardo Valentini;
Riccardo Valentini
Riccardo Valentini in OpenAIREMaurizio Ruzzi;
Maurizio Ruzzi
Maurizio Ruzzi in OpenAIREMaurizio Petruccioli;
Maurizio Petruccioli
Maurizio Petruccioli in OpenAIREAlessandro D’Annibale;
+3 AuthorsAlessandro D’Annibale
Alessandro D’Annibale in OpenAIRERiccardo Valentini;
Riccardo Valentini
Riccardo Valentini in OpenAIREMaurizio Ruzzi;
Maurizio Ruzzi
Maurizio Ruzzi in OpenAIREMaurizio Petruccioli;
Maurizio Petruccioli
Maurizio Petruccioli in OpenAIREAlessandro D’Annibale;
Alessandro D’Annibale
Alessandro D’Annibale in OpenAIREMauro Moresi;
Guglielmo Santi;Mauro Moresi
Mauro Moresi in OpenAIRESilvia Crognale;
Silvia Crognale
Silvia Crognale in OpenAIREOrange peel waste (OPW) was converted into ethanol by consecutive acid-catalysed steam-explosion (ACSE), enzymatic saccharification and fermentation with Saccharomyces cerevisiae F15. The first step was performed in the presence of 0.5% (volume fraction) sulphuric acid at a solid loading of 160 g L−1 using a novel laboratory-scale Direct Steam-Injection Apparatus under different reaction temperatures and times. ACSE at 200 °C for 90 s (or 180 °C for 150 s) yielded the highest solubilization of pectin (∼73%) and a positive effect on the subsequent enzymatic hydrolysis step, that was carried out at 50 °C with an enzyme loading of 6 FPU g−1 cellulose. OPW, pretreated at 180 °C for 150 s, yielded the highest glucose solubilisation degree (∼56%) at the end of the saccharification step, and the maximum ethanol yield coefficient (∼0.495 g g−1) and productivity (4.85 g L−1 h−1) at the end of the 3rd repeated fermentation batch in shaken-flasks.
add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.biombioe.2013.12.007&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eu48 citations 48 popularity Top 10% influence Top 10% impulse Top 10% Powered by BIP!
more_vert add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.biombioe.2013.12.007&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2013 ItalyPublisher:Elsevier BV Authors:Maurizio Petruccioli;
Maurizio Petruccioli
Maurizio Petruccioli in OpenAIREZdena Křesinová;
Zdena Křesinová
Zdena Křesinová in OpenAIREAlessandro D’Annibale;
Alessandro D’Annibale
Alessandro D’Annibale in OpenAIREStefano Covino;
+5 AuthorsStefano Covino
Stefano Covino in OpenAIREMaurizio Petruccioli;
Maurizio Petruccioli
Maurizio Petruccioli in OpenAIREZdena Křesinová;
Zdena Křesinová
Zdena Křesinová in OpenAIREAlessandro D’Annibale;
Alessandro D’Annibale
Alessandro D’Annibale in OpenAIREStefano Covino;
Monika Čvančarová; Monika Čvančarová; Tatiana Stella;Stefano Covino
Stefano Covino in OpenAIRETomáš Cajthaml;
Tomáš Cajthaml;Tomáš Cajthaml
Tomáš Cajthaml in OpenAIREAim of this work was to investigate the ability of Lentinus (Panus) tigrinus to degrade and detoxify a chlorobenzoate (CBA) mixture composed of mono-, di- and tri-chlorinated isomers. The degradation process was investigated as a function of both the growing medium (i.e. low N Kirk's and malt extract-glucose medium) and cultivation conditions (i.e. stationary and shaken cultures). The majority of CBAs were quantitatively degraded within the early 15 d from spiking with the notable exception of the double ortho-chlorinated compounds, 2,6-di-, 2,3,6-tri- and 2,4,6-tri-CBA. Analysis of the degradation intermediates indicated the occurrence of side chain reduction, hydroxylation and methylation reactions. Although CBAs stimulated laccase production, in vitro experiments with a purified L. tigrinus laccase isoenzyme demonstrated its inability to participate in the initial attack on CBAs even in the presence of redox mediators; similar results were found with a Mn-peroxidase isoenzyme. Conversely, prompt degradation was observed upon 1h incubation of CBAs with a purified microsomal fraction containing cytochrome P-450 monooxygenase. The nature of some reaction products (i.e. hydroxylated derivatives), the dependency of the reaction on NADPH and its susceptibility to either CO or piperonyl butoxide inhibition confirmed the involvement of L. tigrinus cytochrome P-450 in the early steps of CBA degradation.
Journal of Hazardous... arrow_drop_down Journal of Hazardous MaterialsArticle . 2013 . Peer-reviewedLicense: Elsevier TDMData sources: Crossrefadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.jhazmat.2013.07.004&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eu14 citations 14 popularity Top 10% influence Average impulse Top 10% Powered by BIP!
more_vert Journal of Hazardous... arrow_drop_down Journal of Hazardous MaterialsArticle . 2013 . Peer-reviewedLicense: Elsevier TDMData sources: Crossrefadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.jhazmat.2013.07.004&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2013 ItalyPublisher:Elsevier BV Authors:Maurizio Petruccioli;
Maurizio Petruccioli
Maurizio Petruccioli in OpenAIREZdena Křesinová;
Zdena Křesinová
Zdena Křesinová in OpenAIREAlessandro D’Annibale;
Alessandro D’Annibale
Alessandro D’Annibale in OpenAIREStefano Covino;
+5 AuthorsStefano Covino
Stefano Covino in OpenAIREMaurizio Petruccioli;
Maurizio Petruccioli
Maurizio Petruccioli in OpenAIREZdena Křesinová;
Zdena Křesinová
Zdena Křesinová in OpenAIREAlessandro D’Annibale;
Alessandro D’Annibale
Alessandro D’Annibale in OpenAIREStefano Covino;
Monika Čvančarová; Monika Čvančarová; Tatiana Stella;Stefano Covino
Stefano Covino in OpenAIRETomáš Cajthaml;
Tomáš Cajthaml;Tomáš Cajthaml
Tomáš Cajthaml in OpenAIREAim of this work was to investigate the ability of Lentinus (Panus) tigrinus to degrade and detoxify a chlorobenzoate (CBA) mixture composed of mono-, di- and tri-chlorinated isomers. The degradation process was investigated as a function of both the growing medium (i.e. low N Kirk's and malt extract-glucose medium) and cultivation conditions (i.e. stationary and shaken cultures). The majority of CBAs were quantitatively degraded within the early 15 d from spiking with the notable exception of the double ortho-chlorinated compounds, 2,6-di-, 2,3,6-tri- and 2,4,6-tri-CBA. Analysis of the degradation intermediates indicated the occurrence of side chain reduction, hydroxylation and methylation reactions. Although CBAs stimulated laccase production, in vitro experiments with a purified L. tigrinus laccase isoenzyme demonstrated its inability to participate in the initial attack on CBAs even in the presence of redox mediators; similar results were found with a Mn-peroxidase isoenzyme. Conversely, prompt degradation was observed upon 1h incubation of CBAs with a purified microsomal fraction containing cytochrome P-450 monooxygenase. The nature of some reaction products (i.e. hydroxylated derivatives), the dependency of the reaction on NADPH and its susceptibility to either CO or piperonyl butoxide inhibition confirmed the involvement of L. tigrinus cytochrome P-450 in the early steps of CBA degradation.
Journal of Hazardous... arrow_drop_down Journal of Hazardous MaterialsArticle . 2013 . Peer-reviewedLicense: Elsevier TDMData sources: Crossrefadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.jhazmat.2013.07.004&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eu14 citations 14 popularity Top 10% influence Average impulse Top 10% Powered by BIP!
more_vert Journal of Hazardous... arrow_drop_down Journal of Hazardous MaterialsArticle . 2013 . Peer-reviewedLicense: Elsevier TDMData sources: Crossrefadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.jhazmat.2013.07.004&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2017 ItalyPublisher:Elsevier BV Authors:Eleonora Carota;
Eleonora Carota
Eleonora Carota in OpenAIRESilvia Crognale;
Silvia Crognale
Silvia Crognale in OpenAIREAlessandro D'Annibale;
Anna Maria Gallo; +2 AuthorsAlessandro D'Annibale
Alessandro D'Annibale in OpenAIREEleonora Carota;
Eleonora Carota
Eleonora Carota in OpenAIRESilvia Crognale;
Silvia Crognale
Silvia Crognale in OpenAIREAlessandro D'Annibale;
Anna Maria Gallo;Alessandro D'Annibale
Alessandro D'Annibale in OpenAIREStazi S;
Maurizio Petruccioli;
Maurizio Petruccioli
Maurizio Petruccioli in OpenAIREThe increasing demand of plant oils for biodiesel production has highlighted the need for alternative strategies based either on non-food crops or agro-industrial wastes that do not compete with food and feed production. In this context, the combined use of wastewater and oleaginous microorganisms could be a valuable production option. Ricotta cheese whey (RCW), one of the major byproducts of the dairy industry, is produced in very high and steadily increasing amounts and, due to its high organic load, its disposal is cost-prohibitive. In the present study, in order to assess the adequacy of RCW as a growth medium for lipid production, 18 strains of oleaginous yeasts were investigated in shaken flask for their growth and lipid-producing capabilities on this substrate. Among them, Cryptococcus curvatus NRRL Y-1511 and Cryptococcus laurentii UCD 68-201 adequately grew therein producing substantial amounts of lipids (6.8 and 5.1gL-1, respectively). A high similarity between the percent fatty acid methyl esters (FAME) composition of lipids from the former and the latter strain was found with a predominance of oleic acid (52.8 vs. 48.7%) and of total saturated fatty acids (37.9 vs. 40.8%). The subsequent scale transfer of the C. laurentii UCD 68-201 lipid production process on RCW to a 3-L STR led to significantly improved biomass and total lipid productions (14.4 and 9.9gL-1, respectively) with the biodiesel yield amounting to 32.6%. Although the C. laurentii FAME profile was modified upon process transfer, it resembled that of the Jatropha oil, a well established feedstock for biodiesel production. In conclusion, C. laurentii UCD 68-201, for which there is very limited amount of available information, turned out to be a very promising candidate for biodiesel production and wide margins of process improvement might be envisaged.
Archivio istituziona... arrow_drop_down Università degli studi della Tuscia: Unitus DSpaceArticle . 2017Full-Text: http://hdl.handle.net/2067/47601Data sources: Bielefeld Academic Search Engine (BASE)The Science of The Total EnvironmentArticle . 2017 . Peer-reviewedLicense: Elsevier TDMData sources: Crossrefadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.scitotenv.2017.01.068&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess RoutesGreen 65 citations 65 popularity Top 1% influence Top 10% impulse Top 10% Powered by BIP!
more_vert Archivio istituziona... arrow_drop_down Università degli studi della Tuscia: Unitus DSpaceArticle . 2017Full-Text: http://hdl.handle.net/2067/47601Data sources: Bielefeld Academic Search Engine (BASE)The Science of The Total EnvironmentArticle . 2017 . Peer-reviewedLicense: Elsevier TDMData sources: Crossrefadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.scitotenv.2017.01.068&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2017 ItalyPublisher:Elsevier BV Authors:Eleonora Carota;
Eleonora Carota
Eleonora Carota in OpenAIRESilvia Crognale;
Silvia Crognale
Silvia Crognale in OpenAIREAlessandro D'Annibale;
Anna Maria Gallo; +2 AuthorsAlessandro D'Annibale
Alessandro D'Annibale in OpenAIREEleonora Carota;
Eleonora Carota
Eleonora Carota in OpenAIRESilvia Crognale;
Silvia Crognale
Silvia Crognale in OpenAIREAlessandro D'Annibale;
Anna Maria Gallo;Alessandro D'Annibale
Alessandro D'Annibale in OpenAIREStazi S;
Maurizio Petruccioli;
Maurizio Petruccioli
Maurizio Petruccioli in OpenAIREThe increasing demand of plant oils for biodiesel production has highlighted the need for alternative strategies based either on non-food crops or agro-industrial wastes that do not compete with food and feed production. In this context, the combined use of wastewater and oleaginous microorganisms could be a valuable production option. Ricotta cheese whey (RCW), one of the major byproducts of the dairy industry, is produced in very high and steadily increasing amounts and, due to its high organic load, its disposal is cost-prohibitive. In the present study, in order to assess the adequacy of RCW as a growth medium for lipid production, 18 strains of oleaginous yeasts were investigated in shaken flask for their growth and lipid-producing capabilities on this substrate. Among them, Cryptococcus curvatus NRRL Y-1511 and Cryptococcus laurentii UCD 68-201 adequately grew therein producing substantial amounts of lipids (6.8 and 5.1gL-1, respectively). A high similarity between the percent fatty acid methyl esters (FAME) composition of lipids from the former and the latter strain was found with a predominance of oleic acid (52.8 vs. 48.7%) and of total saturated fatty acids (37.9 vs. 40.8%). The subsequent scale transfer of the C. laurentii UCD 68-201 lipid production process on RCW to a 3-L STR led to significantly improved biomass and total lipid productions (14.4 and 9.9gL-1, respectively) with the biodiesel yield amounting to 32.6%. Although the C. laurentii FAME profile was modified upon process transfer, it resembled that of the Jatropha oil, a well established feedstock for biodiesel production. In conclusion, C. laurentii UCD 68-201, for which there is very limited amount of available information, turned out to be a very promising candidate for biodiesel production and wide margins of process improvement might be envisaged.
Archivio istituziona... arrow_drop_down Università degli studi della Tuscia: Unitus DSpaceArticle . 2017Full-Text: http://hdl.handle.net/2067/47601Data sources: Bielefeld Academic Search Engine (BASE)The Science of The Total EnvironmentArticle . 2017 . Peer-reviewedLicense: Elsevier TDMData sources: Crossrefadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.scitotenv.2017.01.068&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess RoutesGreen 65 citations 65 popularity Top 1% influence Top 10% impulse Top 10% Powered by BIP!
more_vert Archivio istituziona... arrow_drop_down Università degli studi della Tuscia: Unitus DSpaceArticle . 2017Full-Text: http://hdl.handle.net/2067/47601Data sources: Bielefeld Academic Search Engine (BASE)The Science of The Total EnvironmentArticle . 2017 . Peer-reviewedLicense: Elsevier TDMData sources: Crossrefadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.scitotenv.2017.01.068&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Other literature type , Journal 2020 ItalyPublisher:Elsevier BV Funded by:MIURMIURAuthors:D'Annibale Alessandro;
D'Annibale Alessandro
D'Annibale Alessandro in OpenAIRECarota Eleonora;
Carota Eleonora
Carota Eleonora in OpenAIRECrognale Silvia;
Crognale Silvia
Crognale Silvia in OpenAIREPetruccioli Maurizio;
Petruccioli Maurizio
Petruccioli Maurizio in OpenAIREThe aqueous extraction of orange peel waste (OPW), the byproduct of the juice extraction process generated annually in massive amounts (21 Mton), yields a carbohydrate-rich liquid fraction, termed orange peel extract (OPE). Several studies highlight that the combination of glycerol, a biodiesel byproduct, with carbohydrate mixtures might boost microbial lipid production. This study performed first a shaken flask screening of 15 oleaginous yeast strains based on their growth and lipid-producing abilities on OPE- and glycerol-based media. This screening enabled the selection of R. toruloides NRRL 1091 for the assessment of the process transfer in a stirred tank reactor (STR). This assessment relied, in particular, on either single- and double-stage feeding fed-batch (SSF-FB and DSF-FB, respectively) processes where OPE served as the primary medium and nitrogen-containing glycerol-OPE mixtures as the feeding one. The continuous supply mode at low dilution rates (0.02 and 0.01 h-1 for SSF-FB and DSF-FB, respectively) starting from the end of the exponential growth of the initial batch phase enabled the temporal extension of biomass and lipid production. The SSF-FB and DSF-FB processes attained high biomass and lipid volumetric productions (LVP) and ensured significant lipid accumulation on a dry cell basis (YL/X). The SSF-FB process led to LVP of 20.6 g L-1 after 104 h with volumetric productivity (r L) of 0.20 g L-1 h-1 and YL/X of 0.80; the DSF-FB process yielded LVP, r L and YL/X values equal to 15.92 g L-1, 0.11 g L-1 h-1 and 0.65, respectively. The fatty acid profiles of lipids from both fed-batch processes were not significantly different and resembled that of Jatropha oil, a vastly used feedstock for biodiesel production. These results suggest that OPE constitutes an excellent basis for the fed-batch production of R. toruloides lipids, and this process might afford a further option in OPW-based biorefinery.
Heliyon arrow_drop_down Università degli studi della Tuscia: Unitus DSpaceArticle . 2020Data sources: Bielefeld Academic Search Engine (BASE)Università degli studi della Tuscia: Unitus DSpaceArticle . 2020Data sources: Bielefeld Academic Search Engine (BASE)Università degli studi della Tuscia: Unitus DSpaceArticle . 2020Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.heliyon.2020.e04801&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess RoutesGreen gold 11 citations 11 popularity Top 10% influence Average impulse Top 10% Powered by BIP!
visibility 3visibility views 3 download downloads 2 Powered bymore_vert Heliyon arrow_drop_down Università degli studi della Tuscia: Unitus DSpaceArticle . 2020Data sources: Bielefeld Academic Search Engine (BASE)Università degli studi della Tuscia: Unitus DSpaceArticle . 2020Data sources: Bielefeld Academic Search Engine (BASE)Università degli studi della Tuscia: Unitus DSpaceArticle . 2020Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.heliyon.2020.e04801&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Other literature type , Journal 2020 ItalyPublisher:Elsevier BV Funded by:MIURMIURAuthors:D'Annibale Alessandro;
D'Annibale Alessandro
D'Annibale Alessandro in OpenAIRECarota Eleonora;
Carota Eleonora
Carota Eleonora in OpenAIRECrognale Silvia;
Crognale Silvia
Crognale Silvia in OpenAIREPetruccioli Maurizio;
Petruccioli Maurizio
Petruccioli Maurizio in OpenAIREThe aqueous extraction of orange peel waste (OPW), the byproduct of the juice extraction process generated annually in massive amounts (21 Mton), yields a carbohydrate-rich liquid fraction, termed orange peel extract (OPE). Several studies highlight that the combination of glycerol, a biodiesel byproduct, with carbohydrate mixtures might boost microbial lipid production. This study performed first a shaken flask screening of 15 oleaginous yeast strains based on their growth and lipid-producing abilities on OPE- and glycerol-based media. This screening enabled the selection of R. toruloides NRRL 1091 for the assessment of the process transfer in a stirred tank reactor (STR). This assessment relied, in particular, on either single- and double-stage feeding fed-batch (SSF-FB and DSF-FB, respectively) processes where OPE served as the primary medium and nitrogen-containing glycerol-OPE mixtures as the feeding one. The continuous supply mode at low dilution rates (0.02 and 0.01 h-1 for SSF-FB and DSF-FB, respectively) starting from the end of the exponential growth of the initial batch phase enabled the temporal extension of biomass and lipid production. The SSF-FB and DSF-FB processes attained high biomass and lipid volumetric productions (LVP) and ensured significant lipid accumulation on a dry cell basis (YL/X). The SSF-FB process led to LVP of 20.6 g L-1 after 104 h with volumetric productivity (r L) of 0.20 g L-1 h-1 and YL/X of 0.80; the DSF-FB process yielded LVP, r L and YL/X values equal to 15.92 g L-1, 0.11 g L-1 h-1 and 0.65, respectively. The fatty acid profiles of lipids from both fed-batch processes were not significantly different and resembled that of Jatropha oil, a vastly used feedstock for biodiesel production. These results suggest that OPE constitutes an excellent basis for the fed-batch production of R. toruloides lipids, and this process might afford a further option in OPW-based biorefinery.
Heliyon arrow_drop_down Università degli studi della Tuscia: Unitus DSpaceArticle . 2020Data sources: Bielefeld Academic Search Engine (BASE)Università degli studi della Tuscia: Unitus DSpaceArticle . 2020Data sources: Bielefeld Academic Search Engine (BASE)Università degli studi della Tuscia: Unitus DSpaceArticle . 2020Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.heliyon.2020.e04801&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess RoutesGreen gold 11 citations 11 popularity Top 10% influence Average impulse Top 10% Powered by BIP!
visibility 3visibility views 3 download downloads 2 Powered bymore_vert Heliyon arrow_drop_down Università degli studi della Tuscia: Unitus DSpaceArticle . 2020Data sources: Bielefeld Academic Search Engine (BASE)Università degli studi della Tuscia: Unitus DSpaceArticle . 2020Data sources: Bielefeld Academic Search Engine (BASE)Università degli studi della Tuscia: Unitus DSpaceArticle . 2020Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.heliyon.2020.e04801&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2019 ItalyPublisher:Elsevier BV Authors: Silvia Crognale;Federico Liuzzi;
Federico Liuzzi
Federico Liuzzi in OpenAIREAlessandro D'Annibale;
Alessandro D'Annibale
Alessandro D'Annibale in OpenAIREIsabella de Bari;
+1 AuthorsIsabella de Bari
Isabella de Bari in OpenAIRESilvia Crognale;Federico Liuzzi;
Federico Liuzzi
Federico Liuzzi in OpenAIREAlessandro D'Annibale;
Alessandro D'Annibale
Alessandro D'Annibale in OpenAIREIsabella de Bari;
Isabella de Bari
Isabella de Bari in OpenAIREMaurizio Petruccioli;
Maurizio Petruccioli
Maurizio Petruccioli in OpenAIREhandle: 2067/45702 , 2067/42378 , 2607/42378
Abstract The production of seed oils from Cynara cardunculus generates huge amounts of lignocellulosic residues which can be exploited according to a cascade approach. In this paper, residual cardoon biomass (RCB) was tested as a growth substrate for the solid-state production of cellulolytic cocktails by species known to produce glucose-tolerant β-glucosidase isoenzymes. Best productions were obtained with 10-d-old Aspergillus tubingensis cultures on RCB supplemented with wheat bran (200 g kg−1) yielding β-glucosidase and endo-β-1,4-glucanase activities as high as (25 and 4) IU g−1, respectively, and 4 FPU g−1. The saccharification performance of the obtained cocktail tested on acid-catalysed steam-exploded RCB at low solid loading (25 g dm−3) was around 53% at 20 FPU g−1 cellulose. These performance were significantly enhanced by adding the xylanase-rich NS 22083 commercial formulation, reaching glucose yields higher than 80% after 72 h incubation. The use of the catalytic additive was optimized by a statistical approach, based on factorial analysis. A comparison of the performance of the A. tubingensis reinforced cocktail with the Cellic®CTec2 taken as benchmark formulation was done at the same enzyme load and performed at industrially relevant solid loadings, namely at (100 and 200) g dm−3. This comparison showed that Cellic®CTec2 led to only slightly higher glucose yields while an opposite outcome was observed for xylose yields, irrespective of the solid loading conditions. Thus, this study shows that an in-house enzyme production, based on the solid-state conversion of an industrial byproduct, able of yielding cellulolytic cocktails with substantial saccharification performance is feasible.
Biomass and Bioenerg... arrow_drop_down Università degli studi della Tuscia: Unitus DSpaceArticle . 2019Data sources: Bielefeld Academic Search Engine (BASE)Università degli studi della Tuscia: Unitus DSpaceArticle . 2019Data sources: Bielefeld Academic Search Engine (BASE)Università degli studi della Tuscia: Unitus DSpaceArticle . 2019Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.biombioe.2019.105276&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eu18 citations 18 popularity Top 10% influence Average impulse Top 10% Powered by BIP!
more_vert Biomass and Bioenerg... arrow_drop_down Università degli studi della Tuscia: Unitus DSpaceArticle . 2019Data sources: Bielefeld Academic Search Engine (BASE)Università degli studi della Tuscia: Unitus DSpaceArticle . 2019Data sources: Bielefeld Academic Search Engine (BASE)Università degli studi della Tuscia: Unitus DSpaceArticle . 2019Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.biombioe.2019.105276&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2019 ItalyPublisher:Elsevier BV Authors: Silvia Crognale;Federico Liuzzi;
Federico Liuzzi
Federico Liuzzi in OpenAIREAlessandro D'Annibale;
Alessandro D'Annibale
Alessandro D'Annibale in OpenAIREIsabella de Bari;
+1 AuthorsIsabella de Bari
Isabella de Bari in OpenAIRESilvia Crognale;Federico Liuzzi;
Federico Liuzzi
Federico Liuzzi in OpenAIREAlessandro D'Annibale;
Alessandro D'Annibale
Alessandro D'Annibale in OpenAIREIsabella de Bari;
Isabella de Bari
Isabella de Bari in OpenAIREMaurizio Petruccioli;
Maurizio Petruccioli
Maurizio Petruccioli in OpenAIREhandle: 2067/45702 , 2067/42378 , 2607/42378
Abstract The production of seed oils from Cynara cardunculus generates huge amounts of lignocellulosic residues which can be exploited according to a cascade approach. In this paper, residual cardoon biomass (RCB) was tested as a growth substrate for the solid-state production of cellulolytic cocktails by species known to produce glucose-tolerant β-glucosidase isoenzymes. Best productions were obtained with 10-d-old Aspergillus tubingensis cultures on RCB supplemented with wheat bran (200 g kg−1) yielding β-glucosidase and endo-β-1,4-glucanase activities as high as (25 and 4) IU g−1, respectively, and 4 FPU g−1. The saccharification performance of the obtained cocktail tested on acid-catalysed steam-exploded RCB at low solid loading (25 g dm−3) was around 53% at 20 FPU g−1 cellulose. These performance were significantly enhanced by adding the xylanase-rich NS 22083 commercial formulation, reaching glucose yields higher than 80% after 72 h incubation. The use of the catalytic additive was optimized by a statistical approach, based on factorial analysis. A comparison of the performance of the A. tubingensis reinforced cocktail with the Cellic®CTec2 taken as benchmark formulation was done at the same enzyme load and performed at industrially relevant solid loadings, namely at (100 and 200) g dm−3. This comparison showed that Cellic®CTec2 led to only slightly higher glucose yields while an opposite outcome was observed for xylose yields, irrespective of the solid loading conditions. Thus, this study shows that an in-house enzyme production, based on the solid-state conversion of an industrial byproduct, able of yielding cellulolytic cocktails with substantial saccharification performance is feasible.
Biomass and Bioenerg... arrow_drop_down Università degli studi della Tuscia: Unitus DSpaceArticle . 2019Data sources: Bielefeld Academic Search Engine (BASE)Università degli studi della Tuscia: Unitus DSpaceArticle . 2019Data sources: Bielefeld Academic Search Engine (BASE)Università degli studi della Tuscia: Unitus DSpaceArticle . 2019Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.biombioe.2019.105276&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eu18 citations 18 popularity Top 10% influence Average impulse Top 10% Powered by BIP!
more_vert Biomass and Bioenerg... arrow_drop_down Università degli studi della Tuscia: Unitus DSpaceArticle . 2019Data sources: Bielefeld Academic Search Engine (BASE)Università degli studi della Tuscia: Unitus DSpaceArticle . 2019Data sources: Bielefeld Academic Search Engine (BASE)Università degli studi della Tuscia: Unitus DSpaceArticle . 2019Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.biombioe.2019.105276&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2015Publisher:Springer Science and Business Media LLC Authors:Mauro Moresi;
Mauro Moresi
Mauro Moresi in OpenAIRESilvia Crognale;
Guglielmo Santi;Silvia Crognale
Silvia Crognale in OpenAIREMaurizio Petruccioli;
+2 AuthorsMaurizio Petruccioli
Maurizio Petruccioli in OpenAIREMauro Moresi;
Mauro Moresi
Mauro Moresi in OpenAIRESilvia Crognale;
Guglielmo Santi;Silvia Crognale
Silvia Crognale in OpenAIREMaurizio Petruccioli;
Julia Jasiulewicz;Maurizio Petruccioli
Maurizio Petruccioli in OpenAIREAlessandro D’Annibale;
Alessandro D’Annibale
Alessandro D’Annibale in OpenAIREOrange peel waste was converted into ethanol by consecutive acid-catalyzed steam-explosion (ACSE), enzymatic saccharification, and fermentation with Saccharomyces cerevisiae F15. With the aim of increasing the concentration of fermentable sugars in the hydrolysate and to fully recycle the spent acid liquor as the suspending medium for saccharification, the technical feasibility of increasing the solid loading in the ACSE step from 160 to 480 g L−1 was assessed. At high solid loading in the ACSE pretreatment (HSLAP), the solubilization degrees of polysaccharides were lower while those of potential inhibitors (e.g., acetic and formic acids and phenols) were generally higher than those found at low solid loading (LSLAP). However, residual solids from both solid loadings showed similar susceptibility to enzymatic saccharification (ES) in a 7-L stirred-tank reactor (STR) (cellulase, 12 FPU g−1 cellulose; pectinase, 25 IU g−1 dry matter; 72 h incubation at 50 °C). Fermentation was performed in a 1-L STR along five repeated batches with the first one being used to enable yeast proliferation. By using the hydrolysates arising from the HSLAP-ES combination, it was possible to rely on a fermentation medium with a 2.5-fold higher concentration of simple sugars and to double the ethanol concentration in the final beer to be distilled of. However, the higher content of inhibitory compounds in hydrolysates from HSLAP-ES than in the LSLAP-ES ones led to a reduction in the ethanol yield per unit substrate consumed (0.49 vs. 0.41 g g−1, respectively) and overall productivity (3.4 vs. 2.7 g h−1, respectively).
add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1007/s12155-015-9591-4&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eu17 citations 17 popularity Top 10% influence Average impulse Top 10% Powered by BIP!
more_vert add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1007/s12155-015-9591-4&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2015Publisher:Springer Science and Business Media LLC Authors:Mauro Moresi;
Mauro Moresi
Mauro Moresi in OpenAIRESilvia Crognale;
Guglielmo Santi;Silvia Crognale
Silvia Crognale in OpenAIREMaurizio Petruccioli;
+2 AuthorsMaurizio Petruccioli
Maurizio Petruccioli in OpenAIREMauro Moresi;
Mauro Moresi
Mauro Moresi in OpenAIRESilvia Crognale;
Guglielmo Santi;Silvia Crognale
Silvia Crognale in OpenAIREMaurizio Petruccioli;
Julia Jasiulewicz;Maurizio Petruccioli
Maurizio Petruccioli in OpenAIREAlessandro D’Annibale;
Alessandro D’Annibale
Alessandro D’Annibale in OpenAIREOrange peel waste was converted into ethanol by consecutive acid-catalyzed steam-explosion (ACSE), enzymatic saccharification, and fermentation with Saccharomyces cerevisiae F15. With the aim of increasing the concentration of fermentable sugars in the hydrolysate and to fully recycle the spent acid liquor as the suspending medium for saccharification, the technical feasibility of increasing the solid loading in the ACSE step from 160 to 480 g L−1 was assessed. At high solid loading in the ACSE pretreatment (HSLAP), the solubilization degrees of polysaccharides were lower while those of potential inhibitors (e.g., acetic and formic acids and phenols) were generally higher than those found at low solid loading (LSLAP). However, residual solids from both solid loadings showed similar susceptibility to enzymatic saccharification (ES) in a 7-L stirred-tank reactor (STR) (cellulase, 12 FPU g−1 cellulose; pectinase, 25 IU g−1 dry matter; 72 h incubation at 50 °C). Fermentation was performed in a 1-L STR along five repeated batches with the first one being used to enable yeast proliferation. By using the hydrolysates arising from the HSLAP-ES combination, it was possible to rely on a fermentation medium with a 2.5-fold higher concentration of simple sugars and to double the ethanol concentration in the final beer to be distilled of. However, the higher content of inhibitory compounds in hydrolysates from HSLAP-ES than in the LSLAP-ES ones led to a reduction in the ethanol yield per unit substrate consumed (0.49 vs. 0.41 g g−1, respectively) and overall productivity (3.4 vs. 2.7 g h−1, respectively).
add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1007/s12155-015-9591-4&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eu17 citations 17 popularity Top 10% influence Average impulse Top 10% Powered by BIP!
more_vert add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1007/s12155-015-9591-4&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2014 ItalyPublisher:Elsevier BV Authors: G. Santi;A. D'Annibale;
A. D'Annibale
A. D'Annibale in OpenAIREA. Eshel;
A. Eshel
A. Eshel in OpenAIREA. Zilberstein;
+5 AuthorsA. Zilberstein
A. Zilberstein in OpenAIREG. Santi;A. D'Annibale;
A. D'Annibale
A. D'Annibale in OpenAIREA. Eshel;
A. Eshel
A. Eshel in OpenAIREA. Zilberstein;
A. Zilberstein
A. Zilberstein in OpenAIRES. Crognale;
S. Crognale
S. Crognale in OpenAIREM. Ruzzi;
M. Ruzzi
M. Ruzzi in OpenAIRER. Valentini;
R. Valentini
R. Valentini in OpenAIREM. Moresi;
M. Moresi
M. Moresi in OpenAIREM. Petruccioli;
M. Petruccioli
M. Petruccioli in OpenAIREhandle: 2067/33122 , 2607/33125 , 2607/7104 , 2607/7107 , 2607/33122
Abstract Three different Tamarix species, namely Tamarix aphylla, T. aphylla “Erect”-type and Tamarix jordanis, were grown in an experimental field under extreme desert conditions and irrigated with either reclaimed sewage or brackish water. Depending on both species and source of irrigation, the above-ground biomass production ranged from 18 to 36 Mg ha−1 in the first year. Among the three chemically characterized Tamarix species, T. jordanis was selected due to its higher cellulose content, and lower hemicellulose and phenol contents so as to outline a preliminary process flow sheet for ethanol production. This included steam-injection heating under acidic conditions (200 °C; 90 s; 0.5% H2SO4; 160 g L−1 solid loading) using a novel lab-scale Direct Steam Injection Apparatus, enzymatic saccharification (50 °C; pH 5.0; 200 g L−1 solid loading; 20 FPU g−1 cellulose) and subsequent ethanolic fermentation (30 °C; Saccharomyces cerevisiae F-15 as the inoculum). Lab-scale fermentation runs were carried out in a 3-L stirred bioreactor in repeated-batch mode and showed an almost quantitative conversion of glucose into ethanol (0.507 ± 0.006 g g−1), thus leading to a satisfactory overall process ethanol yield of about 145 L Mg−1 Tamarix biomass.
add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.biombioe.2013.11.024&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eu19 citations 19 popularity Top 10% influence Average impulse Top 10% Powered by BIP!
more_vert add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.biombioe.2013.11.024&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2014 ItalyPublisher:Elsevier BV Authors: G. Santi;A. D'Annibale;
A. D'Annibale
A. D'Annibale in OpenAIREA. Eshel;
A. Eshel
A. Eshel in OpenAIREA. Zilberstein;
+5 AuthorsA. Zilberstein
A. Zilberstein in OpenAIREG. Santi;A. D'Annibale;
A. D'Annibale
A. D'Annibale in OpenAIREA. Eshel;
A. Eshel
A. Eshel in OpenAIREA. Zilberstein;
A. Zilberstein
A. Zilberstein in OpenAIRES. Crognale;
S. Crognale
S. Crognale in OpenAIREM. Ruzzi;
M. Ruzzi
M. Ruzzi in OpenAIRER. Valentini;
R. Valentini
R. Valentini in OpenAIREM. Moresi;
M. Moresi
M. Moresi in OpenAIREM. Petruccioli;
M. Petruccioli
M. Petruccioli in OpenAIREhandle: 2067/33122 , 2607/33125 , 2607/7104 , 2607/7107 , 2607/33122
Abstract Three different Tamarix species, namely Tamarix aphylla, T. aphylla “Erect”-type and Tamarix jordanis, were grown in an experimental field under extreme desert conditions and irrigated with either reclaimed sewage or brackish water. Depending on both species and source of irrigation, the above-ground biomass production ranged from 18 to 36 Mg ha−1 in the first year. Among the three chemically characterized Tamarix species, T. jordanis was selected due to its higher cellulose content, and lower hemicellulose and phenol contents so as to outline a preliminary process flow sheet for ethanol production. This included steam-injection heating under acidic conditions (200 °C; 90 s; 0.5% H2SO4; 160 g L−1 solid loading) using a novel lab-scale Direct Steam Injection Apparatus, enzymatic saccharification (50 °C; pH 5.0; 200 g L−1 solid loading; 20 FPU g−1 cellulose) and subsequent ethanolic fermentation (30 °C; Saccharomyces cerevisiae F-15 as the inoculum). Lab-scale fermentation runs were carried out in a 3-L stirred bioreactor in repeated-batch mode and showed an almost quantitative conversion of glucose into ethanol (0.507 ± 0.006 g g−1), thus leading to a satisfactory overall process ethanol yield of about 145 L Mg−1 Tamarix biomass.
add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.biombioe.2013.11.024&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eu19 citations 19 popularity Top 10% influence Average impulse Top 10% Powered by BIP!
more_vert add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.biombioe.2013.11.024&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2012 ItalyPublisher:Elsevier BV Authors:Federici E.;
Giubilei M. A.;Federici E.
Federici E. in OpenAIRECovino S.;
Covino S.
Covino S. in OpenAIREZANAROLI, GIULIO;
+3 AuthorsZANAROLI, GIULIO
ZANAROLI, GIULIO in OpenAIREFederici E.;
Giubilei M. A.;Federici E.
Federici E. in OpenAIRECovino S.;
Covino S.
Covino S. in OpenAIREZANAROLI, GIULIO;
ZANAROLI, GIULIO
ZANAROLI, GIULIO in OpenAIREFAVA, FABIO;
FAVA, FABIO
FAVA, FABIO in OpenAIRED'Annibale A.;
D'Annibale A.
D'Annibale A. in OpenAIREPetruccioli M.;
Petruccioli M.
Petruccioli M. in OpenAIREObjective of this study was to assess the single or combined effect of a plant oil and a lignocellulosic waste, namely soybean oil (SO) and maize stalks (MS), respectively, on resident microbiota and bioremediation performances of a soil historically contaminated by medium to highly chlorinated PCBs. Higher concentrations of both biphenyl- and chlorobenzoate-degrading cultivable bacteria were found in the MS-amended microcosms (MSM) than the non amended or SO-amended ones after 30 d incubation at 28°C. Fungal growth, instead, was strikingly stimulated in the microcosms that had undergone concomitant MS and SO supplementation (MS-SOM). Denaturing gradient gel electrophoresis analyses of 16S and 18S rRNA genes showed that both amendments promoted a remarkable increase in both bacterial and fungal biodiversity. The abundances of biphenyl-2,3-dioxygenase (bph) and that of catechol-2,3-dioxygenase (C230) genes in the non-amended contaminated soil were constant over time. Conversely, after 60 d incubation, bph and C230 abundances increased 2.8- and 61-fold in the MSM, respectively, and, in the MS-SOM, 1.4- and 46-fold, respectively, with respect to the zero time point. Although the overall PCB removal was not positively affected by the amendments, the concomitant presence of both MS and SO led to significantly higher depletions of hexa-, hepta-, octa- and nona-chlorinated congeners than in the non-amended microcosms (i.e. 24.6, 22, 20.5 and 9.5%, versus 19.4, 16.4, 14.7 and 6.1%, respectively). In all microcosms, PCB degradation was negatively correlated with hydrophobicity, organic matter/water partition coefficient, molecular weight and extent of chlorination of the pollutants with the notable exception of the MS-SOM ones where such a relationship was less stringent.
add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
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For further information contact us at helpdesk@openaire.euAccess Routesgold 23 citations 23 popularity Top 10% influence Average impulse Top 10% Powered by BIP!
more_vert add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
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For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2012 ItalyPublisher:Elsevier BV Authors:Federici E.;
Giubilei M. A.;Federici E.
Federici E. in OpenAIRECovino S.;
Covino S.
Covino S. in OpenAIREZANAROLI, GIULIO;
+3 AuthorsZANAROLI, GIULIO
ZANAROLI, GIULIO in OpenAIREFederici E.;
Giubilei M. A.;Federici E.
Federici E. in OpenAIRECovino S.;
Covino S.
Covino S. in OpenAIREZANAROLI, GIULIO;
ZANAROLI, GIULIO
ZANAROLI, GIULIO in OpenAIREFAVA, FABIO;
FAVA, FABIO
FAVA, FABIO in OpenAIRED'Annibale A.;
D'Annibale A.
D'Annibale A. in OpenAIREPetruccioli M.;
Petruccioli M.
Petruccioli M. in OpenAIREObjective of this study was to assess the single or combined effect of a plant oil and a lignocellulosic waste, namely soybean oil (SO) and maize stalks (MS), respectively, on resident microbiota and bioremediation performances of a soil historically contaminated by medium to highly chlorinated PCBs. Higher concentrations of both biphenyl- and chlorobenzoate-degrading cultivable bacteria were found in the MS-amended microcosms (MSM) than the non amended or SO-amended ones after 30 d incubation at 28°C. Fungal growth, instead, was strikingly stimulated in the microcosms that had undergone concomitant MS and SO supplementation (MS-SOM). Denaturing gradient gel electrophoresis analyses of 16S and 18S rRNA genes showed that both amendments promoted a remarkable increase in both bacterial and fungal biodiversity. The abundances of biphenyl-2,3-dioxygenase (bph) and that of catechol-2,3-dioxygenase (C230) genes in the non-amended contaminated soil were constant over time. Conversely, after 60 d incubation, bph and C230 abundances increased 2.8- and 61-fold in the MSM, respectively, and, in the MS-SOM, 1.4- and 46-fold, respectively, with respect to the zero time point. Although the overall PCB removal was not positively affected by the amendments, the concomitant presence of both MS and SO led to significantly higher depletions of hexa-, hepta-, octa- and nona-chlorinated congeners than in the non-amended microcosms (i.e. 24.6, 22, 20.5 and 9.5%, versus 19.4, 16.4, 14.7 and 6.1%, respectively). In all microcosms, PCB degradation was negatively correlated with hydrophobicity, organic matter/water partition coefficient, molecular weight and extent of chlorination of the pollutants with the notable exception of the MS-SOM ones where such a relationship was less stringent.
add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.nbt.2012.07.007&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess Routesgold 23 citations 23 popularity Top 10% influence Average impulse Top 10% Powered by BIP!
more_vert add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.nbt.2012.07.007&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2010 ItalyPublisher:Elsevier BV Authors:Covino, Stefano;
Svobodová, Kateřina;Covino, Stefano
Covino, Stefano in OpenAIREKřesinová, Zdena;
Křesinová, Zdena
Křesinová, Zdena in OpenAIREPetruccioli, Maurizio;
+4 AuthorsPetruccioli, Maurizio
Petruccioli, Maurizio in OpenAIRECovino, Stefano;
Svobodová, Kateřina;Covino, Stefano
Covino, Stefano in OpenAIREKřesinová, Zdena;
Křesinová, Zdena
Křesinová, Zdena in OpenAIREPetruccioli, Maurizio;
Federici, Federico;Petruccioli, Maurizio
Petruccioli, Maurizio in OpenAIRED'Annibale, Alessandro;
Čvančarová, Monika;D'Annibale, Alessandro
D'Annibale, Alessandro in OpenAIRECajthaml, Tomáš;
Cajthaml, Tomáš
Cajthaml, Tomáš in OpenAIREpmid: 20056409
handle: 11391/1398665 , 2067/35058 , 2607/35058 , 2607/9040
The ability of stationary and shaken Lentinus tigrinus CBS 577.79 liquid cultures to degrade a mixture of polycyclic aromatic hydrocarbons (PAHs) in N-rich (i.e., malt extract glucose, MEG) and in N-limited (low-N Kirk's medium, LNKM) media was investigated. Best results were obtained in shaken cultures where PAHs were degraded by 91% and 97% in MEG and LNKM, respectively; in stationary cultures, on the contrary, the degradation was never higher than 50%. Laccase activity was predominant on MEG while Mn-peroxidase (MnP) was preferentially produced in LNKM. The identification of degradation products showed the presence of several PAH derivatives, such as quinones, dicarboxylated and ring fission derivatives, presumably derived from the action of lignin-modifying enzymes. The presence of some degradation products (e.g., hydroxylated derivatives of anthrone and phenanthrene 9,10-dihydrodiol) suggested the possible involvement of cytochrome P-450-epoxide hydrolase system, the active form of which was found in 7-day-old cultures on MEG. In vitro experiments showed that the MnP from L. tigrinus had wider PAH substrate range and higher oxidation ability than the laccase produced by the same strain.
add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.biortech.2009.12.020&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eu53 citations 53 popularity Top 10% influence Top 10% impulse Top 10% Powered by BIP!
more_vert add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.biortech.2009.12.020&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2010 ItalyPublisher:Elsevier BV Authors:Covino, Stefano;
Svobodová, Kateřina;Covino, Stefano
Covino, Stefano in OpenAIREKřesinová, Zdena;
Křesinová, Zdena
Křesinová, Zdena in OpenAIREPetruccioli, Maurizio;
+4 AuthorsPetruccioli, Maurizio
Petruccioli, Maurizio in OpenAIRECovino, Stefano;
Svobodová, Kateřina;Covino, Stefano
Covino, Stefano in OpenAIREKřesinová, Zdena;
Křesinová, Zdena
Křesinová, Zdena in OpenAIREPetruccioli, Maurizio;
Federici, Federico;Petruccioli, Maurizio
Petruccioli, Maurizio in OpenAIRED'Annibale, Alessandro;
Čvančarová, Monika;D'Annibale, Alessandro
D'Annibale, Alessandro in OpenAIRECajthaml, Tomáš;
Cajthaml, Tomáš
Cajthaml, Tomáš in OpenAIREpmid: 20056409
handle: 11391/1398665 , 2067/35058 , 2607/35058 , 2607/9040
The ability of stationary and shaken Lentinus tigrinus CBS 577.79 liquid cultures to degrade a mixture of polycyclic aromatic hydrocarbons (PAHs) in N-rich (i.e., malt extract glucose, MEG) and in N-limited (low-N Kirk's medium, LNKM) media was investigated. Best results were obtained in shaken cultures where PAHs were degraded by 91% and 97% in MEG and LNKM, respectively; in stationary cultures, on the contrary, the degradation was never higher than 50%. Laccase activity was predominant on MEG while Mn-peroxidase (MnP) was preferentially produced in LNKM. The identification of degradation products showed the presence of several PAH derivatives, such as quinones, dicarboxylated and ring fission derivatives, presumably derived from the action of lignin-modifying enzymes. The presence of some degradation products (e.g., hydroxylated derivatives of anthrone and phenanthrene 9,10-dihydrodiol) suggested the possible involvement of cytochrome P-450-epoxide hydrolase system, the active form of which was found in 7-day-old cultures on MEG. In vitro experiments showed that the MnP from L. tigrinus had wider PAH substrate range and higher oxidation ability than the laccase produced by the same strain.
add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.biortech.2009.12.020&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eu53 citations 53 popularity Top 10% influence Top 10% impulse Top 10% Powered by BIP!
more_vert add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.biortech.2009.12.020&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eu